上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
ReadiView 绿色/红色 细胞活性染料
 |
货号 | 22763 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 1000 Tests | 价格 | 6060 | |
| Ex (nm) | Em (nm) | |||
| 分子量 | 溶剂 | Water | ||
| 产品详细介绍 | ||||
简要概述
ReadiView 绿色/红色活性染料是一种耐用的细胞染色剂，经过优化，可用于对Countess II FL或Countess 3 FL仪器中的有核细胞进行染色。 它可以方便地与传统的FITC或GFP滤光片组配合使用，例如EVOS GFP荧光灯适用于激发和检测绿色染料，而RFP或Texas Red（TxR）荧光灯适用于细胞红染色。 绿色表示活细胞，而红色表示死细胞。 ReadiView染料可对混合群体中的有核细胞进行特定染色，从而降低了复杂性，并在与适当的EVOS光学立方体匹配时提高了计数与样品间的可重复性。
产品说明书
样本实验方案
将10 µL ReadyCount 活/死活力染色剂与10 µL细胞混合。
在室温下孵育2至5分钟。
将10 µL染色的细胞样品上样至Countess细胞计数仪的玻片中。
在Countess细胞计数仪上读取数据。
图示
图1.用ReadyView 绿色/红色细胞活性染料标记的Jurkat细胞的荧光图像。
参考文献
Drug combinations against Borrelia burgdorferi persisters in vitro: eradication achieved by using daptomycin, cefoperazone and doxycycline.
Authors: Feng, Jie and Auwaerter, Paul G and Zhang, Ying
Journal: PloS one (2015): e0117207
Growth inhibition of cultured marine phytoplankton by toxic algal-derived polyunsaturated aldehydes.
Authors: Ribalet, François and Berges, John A and Ianora, Adrianna and Casotti, Raffaella
Journal: Aquatic toxicology (Amsterdam, Netherlands) (2007): 219-27
An improved method for the selective detection of fungi in hospital waters by solid phase cytometry.
Authors: De Vos, Muriel M and Nelis, Hans J
Journal: Journal of microbiological methods (2006): 557-65
A membrane-immunofluorescent-viability staining technique for the detection of Salmonella spp. from fresh and processed meat samples.
Authors: Duffy, G and Kilbride, B and Sheridan, J J and Blair, I S and McDowell, D A
Journal: Journal of applied microbiology (2000): 587-94
Staining with fluorescein diacetate correlates with hepatocyte function.
Authors: Nyberg, S L and Shatford, R A and Payne, W D and Hu, W S and Cerra, F B
Journal: Biotechnic & histochemistry : official publication of the Biological Stain Commission (1993): 56-63
