TAQuest qPCR Master Mix 用于TaqMan探针*低ROX* 货号17285-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest qPCR Master Mix 用于TaqMan探针*低ROX*

TAQuest qPCR Master Mix 用于TaqMan探针*低ROX*

TAQuest qPCR Master Mix 用于TaqMan探针*低ROX*    货号17285 货号 17285 存储条件 在零下15度以下保存, 避免光照
规格 5 mL 价格 3612
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17285

产品名称:TAQuest qPCR Master Mix 用于TaqMan探针*低ROX*

规格:5ml

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

溶剂:水

 

产品介绍

用于 TaqMan 探针的 TAQuest qPCR Master Mix 是一种即用型 2X溶液,针对 qPCR 和与 TaqMan 基因表达分析兼容的两步法 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中提供了所有基本成分,包括我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP,但模板、引物和探针除外。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应,从而最大限度地减少非特异性产物的形成。优化的成分可确保 PCR 对所有样本类型(如基因组、质粒、病毒和 cDNA 模板)的特异性和灵敏度。用于 TaqMan 探针的 TAQuest qPCR Master Mix 设计用于使用内部阳性对照的双链反应。该预混液含少量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest qPCR Master Mix 用于TaqMan探针*低ROX*。 

 

适用仪器


qPCR  
仪器规格 基于探针的滤波片

 

样品实验方案
注意 在室温下解冻TAQuest qPCR Master Mix 用于TaqMan探针*低ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest qPCR Master Mix 用于TaqMan探针*低ROX* 12.5 µL 25 µL 1X
上游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.
Authors: Lista, Maria Jose and Matos, Pedro M and Maguire, Thomas J A and Poulton, Kate and Ortiz-Zapater, Elena and Page, Robert and Sertkaya, Helin and Ortega-Prieto, Ana M and Scourfield, Edward and O’Byrne, Aoife M and Bouton, Clement and Dickenson, Ruth E and Ficarelli, Mattia and Jimenez-Guardeño, Jose M and Howard, Mark and Betancor, Gilberto and Galao, Rui Pedro and Pickering, Suzanne and Signell, Adrian W and Wilson, Harry and Cliff, Penelope and Kia Ik, Mark Tan and Patel, Amita and MacMahon, Eithne and Cunningham, Emma and Doores, Katie and Agromayor, Monica and Martin-Serrano, Juan and Perucha, Esperanza and Mischo, Hannah E and Shankar-Hari, Manu and Batra, Rahul and Edgeworth, Jonathan and Zuckerman, Mark and Malim, Michael H and Neil, Stuart and Martinez-Nunez, Rocio Teresa
Journal: PloS one (2021): e0256813

Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.
Authors: Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin
Journal: Journal of virological methods (2019): 58-64

Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9

Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR.
Authors: Schrell, Adrian M and Roper, Michael G
Journal: The Analyst (2014): 2695-701

Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
Authors: Rombach, Markus and Kosse, Dominique and Faltin, Bernd and Wadle, Simon and Roth, Günter and Zengerle, Roland and von Stetten, Felix
Journal: BioTechniques (2014): 151-5

Development of a novel internal positive control for Taqman based assays.
Authors: Hartman, Laurie J and Coyne, Susan R and Norwood, David A
Journal: Molecular and cellular probes (2005): 51-9

[Establishment and application of real-time fluorescence polymerase chain reaction based on the TaqMan probes for detection of Yersinia pestis].
Authors: Li, Wei and Hai, Rong and Yu, Dong-zheng and Zhang, Zhi-kai and Cai, Hong
Journal: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi (2005): 613-6

[Multiplex PCR for detection and quantification of GM potato event EH92-527-1 in food].
Authors: Tyshko, N V and Sadykova, E O and Grouzdev, D S and Sukhacheva, M V
Journal: Voprosy pitaniia: 57-61

[Multiplex polymerase chain reaction for genetically modified potato event AV43-6-G7 quantification. Proof of efficiency].
Authors: Tyshko, N V and Sadykova, E O and Sukhacheva, M V and Grouzdev, D S
Journal: Voprosy pitaniia: 62-70

说明书
TAQuest qPCR Master Mix 用于TaqMan探针*低ROX*.pdf

TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX* 货号17290-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*

TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*

TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*    货号17290 货号 17290 存储条件 在零下15度以下保存, 避免光照
规格 1 mL 价格 1164
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17290

产品名称:TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*

规格:1ml

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

溶剂:水

 

产品介绍

用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 是一种即用型 2X 溶液,针对 qPCR 和两步法 RT-qPCR 进行了优化,非常适合用于 TaqMan 基因表达分析。预混液与 FAST 条件兼容,因此在 20 uL 反应体积中进行 40 个 PCR 循环,可在 50 分钟内提供结果。预混液提供所有基本成分,包括我们专有的 TAQuest FAST 热启动 Taq DNA 聚合酶和优化的 PCR 缓冲液中的 dNTP,但模板、引物和探针除外。用于 TaqMan 探针的 TAQuest FAST qPCR Master Mix 设计用于使用具有卓越性能的内部阳性对照进行双重反应。预混液可确保所有样品类型(如基因组、质粒、病毒和 cDNA 模板)的 PCR 特异性和灵敏度。该预混液含少量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*。

 

适用仪器


qPCR  
仪器规格 基于探针的滤波片

 

样品实验方案
注意 在室温下解冻TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX* 12.5 µL 25 µL 1X
上游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Evaluation of the efficiency of TaqMan duplex real-time PCR assay for non-invasive pre-natal assessment of foetal sex in equine.
Authors: Kadivar, Ali and Rashidzadeh, Habiballah and Davoodian, Najmeh and Nazari, Hasan and Dehghani Tafti, Rohallah and Heidari Khoei, Heidar and Seidi Samani, Hasan and Modaresi, Jahangir and Ahmadi, Ebrahim
Journal: Reproduction in domestic animals = Zuchthygiene (2021): 287-291

Fast and Sensitive Real-Time PCR Detection of Major Antiviral-Drug Resistance Mutations in Chronic Hepatitis B Patients by Use of a Predesigned Panel of Locked-Nucleic-Acid TaqMan Probes.
Authors: Chu, Son V and Vu, Son T and Nguyen, Hang M and Le, Ngan T and Truong, Phuong T and Vu, Van T T and Phung, Thuy T B and Nguyen, Anh T V
Journal: Journal of clinical microbiology (2021): e0093621

Multiplex TaqMan Real-Time PCR Assay for Sensitive Detection of Two Weevil Species (Coleoptera: Curculionidae).
Authors: Aguirre, Carlos and Sánchez, Evelyn and Olivares, Natalia and Hinrichsen, Patricio
Journal: Journal of economic entomology (2021): 90-99

A TaqMan-based quantitative real-time PCR assay for identification of the goose circovirus.
Authors: Yang, Kan-Kan and Yin, Dong-Dong and Xu, Liang and Liang, Yue-Qiao and Tu, Jian and Song, Xiang-Jun and Shao, Ying and Liu, Hong-Mei and Qi, Ke-Zong
Journal: Molecular and cellular probes (2020): 101564

A development strategy to fast establish the Taqman qPCR based method to detect SNP mutations.
Authors: Jiang, Xiaohui and Xiang, Junbei and Wang, Ruifeng and Wan, Qian
Journal: Human cell (2020): 1331-1333

BlueTYPE – A low density TaqMan-RT-qPCR array for the identification of all 24 classical Bluetongue virus serotypes.
Authors: Ries, Christina and Beer, Martin and Hoffmann, Bernd
Journal: Journal of virological methods (2020): 113881

Brief comparative evaluation of six open one-step RT-qPCR mastermixes for the detection of SARS-CoV-2 RNA using a Taqman probe.
Authors: Haddar, Cyrille and Verhoeven, Paul O and Bourlet, Thomas and Pozzetto, Bruno and Pillet, Sylvie
Journal: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology (2020): 104636

Development of a new TaqMan-based PCR assay for the specific detection and quantification of Simkania negevensis.
Authors: Baccari, Olfa and Elleuch, Jihen and Barkallah, Mohamed and Boukedi, Hanen and Ayed, Nourelhouda Ben and Hammami, Adnene and Fendri, Imen and Abdelkafi, Slim
Journal: Molecular and cellular probes (2020): 101645

One-step multiplex TaqMan probe-based method for real-time PCR detection of four canine diarrhea viruses.
Authors: Wang, Ruyi and Zhang, Wenyan and Ye, Rui and Pan, Zhongzhou and Li, Gairu and Su, Shuo
Journal: Molecular and cellular probes (2020): 101618

Quantification of Major Bacteria and Yeast Species in Kefir Consortia by Multiplex TaqMan qPCR.
Authors: Nejati, Fatemeh and Junne, Stefan and Kurreck, Jens and Neubauer, Peter
Journal: Frontiers in microbiology (2020): 1291

说明书
TAQuest FAST qPCR Master Mix 用于TaqMan探针*低ROX*.pdf

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX* 货号17277-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*

TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*    货号17277 货号 17277 存储条件 在零下15度以下保存, 避免光照
规格 5 mL 价格 3612
Ex (nm) Em (nm)
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:17277

产品名称:TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*

规格:5ml

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

溶剂:水

 

产品介绍

TAQuest FAST qPCR Master Mix with Helixyte Green 是一种即用型 2X溶液,针对 qPCR 和两步法 RT-qPCR 进行了优化。对于 20 uL 反应体积中的 40 个 PCR 循环,预混液可在 50 分钟内提供结果。该混合物包括含有 dNTP 的优化缓冲液和我们专有的 TAQuest FAST 热启动 Taq DNA 聚合酶,该酶旨在允许即时热启动,最大限度地减少非特异性产物的形成,从而允许室温反应设置。运行所需的 PCR 反应只需要模板和目标引物。 TAQuest FAST qPCR Master Mix 与 Helixyte Green 确保 PCR 特异性和灵敏度对所有样品类型,如基因组、质粒、病毒和 cDNA 模板。 Helixyte Green 嵌入染料无需使用序列特异性探针即可快速检测和分析 DNA。该预混液不含 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*。 

 

适用仪器


qPCR  
仪器规格 SYBR Green 滤波片

 

样品实验方案
注意 在室温下用 Helixyte Green *无ROX* 解冻 TAQuest™ FAST qPCR Master Mix。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂,然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

 

表 1. 各反应每孔试剂组成

成分 体积 (25 µL/reaction) 体积 (50 µL/reaction) 最终浓度
TAQuest qPCR Master Mix with Helixyte Green *无 ROX* 12.5 µL 25 µL 1X
上游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
下游引物,10 µM 0.25-2.5 µL 0.5-5.0 µL 0.1-1.0 µM
DNA模板 1-5 µL 1-5 µL 优化的浓度
无核酸酶水 25 µL 50     µL  

表 2. 热循环参数

范围 聚合酶激活 PCR (30-40个循环)
  Hold 变性 退火 延伸
温度 95 °C 95 °C 55-65 °C 68-72 °C
时间 (m:ss) 0:20 0:30 1:00 1:00

 

参考文献

Fatal systemic toxoplasmosis in a 3-month-old young tibetan goat (Capra hircus).
Authors: Pavone, Silvia and Crotti, Silvia and Cruciani, Deborah and D’Avino, Nicoletta and Zema, Jacopo and Morelli, Simone and Gobbi, Marco and Madeo, Laura
Journal: BMC veterinary research (2020): 423

Development of four PCR-based methods to differentiate tilefish species (Branchiostegus japonicus and B. albus).
Authors: Kang, Tae Sun
Journal: Food chemistry (2019): 1-8

A multi-screening Fast qPCR approach to the identification of abortive agents in ruminants.
Authors: Sebastiani, Carla and Curcio, Ludovica and Ciullo, Marcella and Cruciani, Deborah and Crotti, Silvia and Pesca, Cristina and Torricelli, Martina and Sebastianelli, Martina and Felici, Andrea and Biagetti, Massimo
Journal: Journal of microbiological methods (2018): 12-17

A rapid real-time PCR method to differentiate between mottled skate (Beringraja pulchra) and other skate and ray species.
Authors: Kim, Mi-Ra and Kwon, Kisung and Jung, Yoo-Kyung and Kang, Tae Sun
Journal: Food chemistry (2018): 112-119

Development of a Sensitive Real-Time Fast-qPCR Based on SYBR® Green for Detection and Quantification of Chicken Parvovirus (ChPV).
Authors: Nuñez, Luis F and Santander-Parra, Silvana H and Chaible, Lucas and De la Torre, David I and Buim, Marcos R and Murakami, Alexandre and Zaidan Dagli, Maria Lucia and Astolfi-Ferreira, Claudete S and Piantino Ferreira, Antonio J
Journal: Veterinary sciences (2018)

Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9

Fast quantitative PCR, locked nucleic acid probes and reduced volume reactions are effective tools for detecting Batrachochytrium dendrobatidis DNA.
Authors: Ruthig, Gregory R and Deridder, Benjamin P
Journal: Diseases of aquatic organisms (2012): 249-53

Multi-platform comparison of ten commercial master mixes for probe-based real-time polymerase chain reaction detection of bioterrorism threat agents for surge preparedness.
Authors: Buzard, Gregory S and Baker, Daniel and Wolcott, Mark J and Norwood, David A and Dauphin, Leslie A
Journal: Forensic science international (2012): 292-7

Real-time quantitative PCR and fast QPCR have similar sensitivity and accuracy with HIV cDNA late reverse transcripts and 2-LTR circles.
Authors: Yoder, Kristine E and Fishel, Richard
Journal: Journal of virological methods (2008): 253-6

Detection of equine herpesvirus-1 in nasal swabs of horses by quantitative real-time PCR.
Authors: Perkins, G A and Goodman, L B and Dubovi, E J and Kim, S G and Osterrieder, N
Journal: Journal of veterinary internal medicine: 1234-8

说明书
TAQuest FAST qPCR Master Mix with Helixyte Green *无ROX*.pdf

FAM 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67006-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

FAM 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

FAM 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

FAM 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*    货号67006 货号 67006 存储条件 在零下15度以下保存, 避免光照
规格 1 Plate 价格 1140
Ex (nm) Em (nm)
分子量 溶剂 DMSO
产品详细介绍

简要概述

FAM 染料 qPCR 校准板可用于维护配备快速 96 孔板的 7500 实时 PCR 系统。 对于大多数 qPCR 仪器,必要的校准应至少每六个月进行一次。 该校准板可以立即使用,无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。

 

参考文献

Novel design of nucleic acid standards for hydrolysis probe-based PCR with melting analysis.
Authors: Baoutina, Anna and Bhat, Somanath
Journal: Gene therapy (2021)
Determination of Advantages and Limitations of qPCR Duplexing in a Single Fluorescent Channel.
Authors: Zhang, Haoqing and Yan, Zhiqiang and Wang, Xinlu and Gaňová, Martina and Chang, Honglong and Laššáková, Soňa and Korabecna, Marie and Neuzil, Pavel
Journal: ACS omega (2021): 22292-22300
SARS-CoV-2 Detection using Real Time PCR by a Commercial Diagnostic Kit.
Authors: Roy, S and Paul, S K and Barman, T K and Ahmed, S and Haque, N and Mazid, R and Debnath, P and Roy, S A
Journal: Mymensingh medical journal : MMJ (2020): 596-600
Fluorescent Molecular Beacons Mimicking RNA Secondary Structures to Study RNA Chaperone Activity.
Authors: Menendez-Gil, Pilar and Caballero, Carlos J and Solano, Cristina and Toledo-Arana, Alejandro
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 41-58
Development and Evaluation of a Single Dye Duplex Droplet Digital PCR Assay for the Rapid Detection and Quantification of Mycobacterium tuberculosis.
Authors: Nyaruaba, Raphael and Xiong, Jin and Mwaliko, Caroline and Wang, Nuo and Kibii, Belindah J and Yu, Junping and Wei, Hongping
Journal: Microorganisms (2020)
A handheld continuous-flow real-time fluorescence qPCR system with a PVC microreactor.
Authors: Shi, Bing and Li, Yuanming and Wu, Di and Wu, Wenming
Journal: The Analyst (2020): 2767-2773
Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions.
Authors: Tomé, Stéphanie and Gourdon, Geneviève
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 11-23
Ratiometric fluorescence sensor based on carbon dots as internal reference signal and T7 exonuclease-assisted signal amplification strategy for microRNA-21 detection.
Authors: Wang, Zhenzhen and Xue, Zhiqiang and Hao, Xiaoli and Miao, Chenfang and Zhang, Jianzhong and Zheng, Yanjie and Zheng, Zongfu and Lin, Xinhua and Weng, Shaohuang
Journal: Analytica chimica acta (2020): 212-219
PharmFrag: An Easy and Fast Multiplex Pharmacogenetics Assay to Simultaneously Analyze 9 Genetic Polymorphisms Involved in Response Variability of Anticancer Drugs.
Authors: Bouvet, Régis and Verdier, Marie-Clémence and El Baroudi, Yahya and Galibert, Marie-Dominique and David, Véronique and Schutz, Sacha and Tron, Camille
Journal: International journal of molecular sciences (2020)
The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR.
Authors: Liu, Jinding and Wang, Rongshuai and Shi, Jie and Cheng, Xiaojuan and Hao, Ting and Guo, Jiangling and Wang, Jiaqi and Liu, Zidong and Li, Wenyan and Fan, Haoliang and Yun, Keming and Yan, Jiangwei and Zhang, Gengqian
Journal: International journal of legal medicine (2020): 2015-2027

说明书
FAM 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*.pdf

JOE 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化* 货号67012-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

JOE 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

JOE 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*

JOE 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*    货号67012 货号 67012 存储条件 在零下15度以下保存, 避免光照
规格 1 Plate 价格 1140
Ex (nm) Em (nm)
分子量 溶剂 DMSO
产品详细介绍

简要概述

JOE 染料 qPCR 校准板可用于维护配备快速 96 孔板的 7500 实时 PCR 系统。 对于大多数 qPCR 仪器,必要的校准应至少每六个月进行一次。 该校准板可以立即使用,无需任何额外的准备步骤。 qPCR 校准板可以通过更准确地表示实时实验中使用的荧光光谱来改善多路复用的 qPCR 结果。

 

参考文献

The construction and application of a new 17-plex Y-STR system using universal fluorescent PCR.
Authors: Liu, Jinding and Wang, Rongshuai and Shi, Jie and Cheng, Xiaojuan and Hao, Ting and Guo, Jiangling and Wang, Jiaqi and Liu, Zidong and Li, Wenyan and Fan, Haoliang and Yun, Keming and Yan, Jiangwei and Zhang, Gengqian
Journal: International journal of legal medicine (2020): 2015-2027
Detection of simultaneous multi-mutations using base-quenched probe.
Authors: Mao, Huihui and Luo, Guanghua and Zhang, Jun and Xu, Ning
Journal: Analytical biochemistry (2018): 79-81
Molecular beacons with JOE dye: Influence of linker and 3′ couple quencher.
Authors: Tsybulsky, Dmitry A and Kvach, Maksim V and Ryazantsev, Dmitry Y and Aparin, Ilya O and Stakheev, Alexander A and Prokhorenko, Igor A and Martynenko, Yury V and Gontarev, Sergey V and Formanovsky, Andrey A and Zatsepin, Timofei S and Shmanai, Vadim V and Korshun, Vladimir A and Zavriev, Sergey K
Journal: Molecular and cellular probes (2016): 285-290
Mineralization Effect of Hyaluronan on Dental Pulp Cells via CD44.
Authors: Chen, Kuan-Liang and Yeh, Ying-Yi and Lung, Jrhau and Yang, Yu-Chi and Yuan, Kuo
Journal: Journal of endodontics (2016): 711-6
Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay.
Authors: Maksyutov, Rinat A and Gavrilova, Elena V and Shchelkunov, Sergei N
Journal: Journal of virological methods (2016): 215-220
Solid- and solution-phase synthesis and application of R6G dual-labeled oligonucleotide probes.
Authors: Skoblov, Aleksander Yu and Vichuzhanin, Maxim V and Farzan, Valentina M and Veselova, Olga A and Konovalova, Tatiana A and Podkolzin, Alexander T and Shipulin, German A and Zatsepin, Timofei S
Journal: Bioorganic & medicinal chemistry (2015): 6749-56
Combined effects of simvastatin and enamel matrix derivative on odontoblastic differentiation of human dental pulp cells.
Authors: Karanxha, Lorena and Park, Su-Jung and Son, Won-Jun and Nör, Jacques E and Min, Kyung-San
Journal: Journal of endodontics (2013): 76-82
In vivo quantitative evaluation of live and dead bacteria in root canal infection by using propidium monoazide with real-time PCR.
Authors: Kim, Sin-Young and Shin, Yooseok and Lee, Chan-Young and Jung, Il-Young
Journal: Journal of endodontics (2013): 1359-63
Two-dye and one- or two-quencher DNA probes for real-time PCR assay: synthesis and comparison with a TaqMan™ probe.
Authors: Ryazantsev, Dmitry Y and Tsybulsky, Dmitry A and Prokhorenko, Igor A and Kvach, Maksim V and Martynenko, Yury V and Philipchenko, Pavel M and Shmanai, Vadim V and Korshun, Vladimir A and Zavriev, Sergey K
Journal: Analytical and bioanalytical chemistry (2012): 59-68
ADAM28 manipulates proliferation, differentiation, and apoptosis of human dental pulp stem cells.
Authors: Zhao, Zheng and Liu, Hongchen and Wang, Dongsheng
Journal: Journal of endodontics (2011): 332-9

说明书
JOE 染料 qPCR 校准板 *针对 ABI7500 快速 96 孔进行了优化*.pdf