钙离子荧光探针Oregon Green 488 BAPTA-1,六钾盐 货号20506-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

钙离子荧光探针Oregon Green 488 BAPTA-1,六钾盐

钙离子荧光探针Oregon Green 488 BAPTA-1,六钾盐

钙离子荧光探针Oregon Green 488 BAPTA-1,六钾盐    货号20506 货号 20506 存储条件 在零下15度以下保存, 避免光照
规格 500 ug 价格 2604
Ex (nm) 493 Em (nm) 522
分子量 1114.28 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:20506

产品名称:钙离子荧光探针Oregon Green 488 BAPTA-1,六钾盐

规格:500ug

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:1114.28

溶剂:水

激发波长(nm):493

发射波长(nm):522

 

产品介绍

钙离子荧光探针Oregon Green 488 BAPTA-1,六钾盐是美国AAT Bioquest生产的钙离子荧光探针,OG488 BAPTA -1与Oregon Green 488 BAPTA-1的分子相同。 它是一种可见光可激发的钙指示剂,通常与FITC过滤器组配合使用。 OG 488 BAPTA-1的激发和发射分别为494和523 nm。 结合钙离子后其荧光强度增加~14倍。 这种水溶性盐形式可用于通过显微注射或从贴片移液管输注的细胞内负载。 也可获得指示剂的细胞渗透性AM酯形式(#20507)。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的钙离子荧光探针Oregon Green 488 BAPTA-1,六钾盐。 金畔生物是AAT Bioquest的中国代理商,为您提供最优质的荧光素。 

点击查看光谱

钙离子篇:时间轴式讲解应用于钙离子检测的探针

 

参考文献

Norepinephrine-induced calcium signaling in astrocytes in the respiratory network of the ventrolateral medulla
Authors: Schnell C, Negm M, Driehaus J, Scheller A, Hulsmann S.
Journal: Respir Physiol Neurobiol (2016): 18

Optical electrocorticogram (OECoG) using wide-field calcium imaging reveals the divergence of neuronal and glial activity during acute rodent seizures
Authors: Daniel AG, Laffont P, Zhao M, Ma H, Schwartz TH.
Journal: Epilepsy Behav (2015): 61

Two-Photon Processor and SeNeCA: a freely available software package to process data from two-photon calcium imaging at speeds down to several milliseconds per frame
Authors: Tomek J, Novak O, Syka J.
Journal: J Neurophysiol (2013): 243

Calcium buffering and clearance in spider mechanosensory neurons
Authors: Schmitz J, Hoger U, Torkkeli PH, French AS.
Journal: J Comp Physiol A Neuroethol Sens Neural Behav Physiol (2012): 477

Post hoc immunostaining of GABAergic neuronal subtypes following in vivo two-photon calcium imaging in mouse neocortex
Authors: Langer D, Helmchen F.
Journal: Pflugers Arch (2012): 339

Astrocyte calcium signaling transforms cholinergic modulation to cortical plasticity in vivo
Authors: Takata N, Mishima T, Hisatsune C, Nagai T, Ebisui E, Mikoshiba K, Hirase H.
Journal: J Neurosci (2011): 18155

Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377

Multifocal animated imaging of changes in cellular oxygen and calcium concentrations and membrane potential within the intact adult mouse carotid body ex vivo
Authors: Wotzlaw C, Bernardini A, Berchner-Pfannschmidt U, Papkovsky D, Acker H, F and rey J.
Journal: Am J Physiol Cell Physiol (2011): C266

Somatic depolarization enhances GABA release in cerebellar interneurons via a calcium/protein kinase C pathway
Authors: Bouhours B, Trigo FF, Marty A.
Journal: J Neurosci (2011): 5804

Mitochondrial DNA mutations affect calcium handling in differentiated neurons
Authors: Trevelyan AJ, Kirby DM, Smulders-Srinivasan TK, Nooteboom M, Acin-Perez R, Enriquez JA, Whittington MA, Lightowlers RN, Turnbull DM.
Journal: Brain (2010): 787

说明书
钙离子荧光探针Oregon Green 488 BAPTA-1,六钾盐.pdf

5-OG488 酸(相当于Oregon Green 488 羧酸,5-异构体) 货号708-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

5-OG488 酸(相当于Oregon Green 488 羧酸,5-异构体)

5-OG488 酸(相当于Oregon Green 488 羧酸,5-异构体)

5-OG488 酸(相当于Oregon Green 488 羧酸,5-异构体)    货号708 货号 708 存储条件 在零下15度以下保存, 避免光照
规格 25 mg 价格 2604
Ex (nm) 498 Em (nm) 526
分子量 412.30 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:708

产品名称:5-OG488 酸

规格:25mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:412.30

溶剂:DMSO

激发波长(nm):498

发射波长(nm):526

 

产品介绍

5-OG488 酸是美国AAT Bioquest生产的荧光染料。5-OG488酸与OregonGreen®488羧酸,5-异构体(OregonGreen®是Invitrogen的商标)是相同的分子。 这种化合物具有较高的光稳定性和较低的pKa~4.7,而5-FAM具有pKa~6.4。 5-OG488的pKa降低使其荧光在生理pH范围内基本上对pH不敏感。 该胺反应性5-OG488可用于制备亮绿色荧光生物共轭物,其激发/发射最大值为~496 / 524nm。 它是5-FITC的极好替代品。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的5-OG488 酸。 

点击查看光谱

 

参考文献

Biodegradable polymersomes as carriers and release systems for paclitaxel using Oregon Green(R) 488 labeled paclitaxel as a model compound
Authors: Lee JS, Feijen J.
Journal: J Control Release (2012): 312

Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377

Pharmacokinetics of intraocular drug delivery of Oregon green 488-labeled triamcinolone by subtenon injection using ocular fluorophotometry in rabbit eyes
Authors: Lee SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: Invest Ophthalmol Vis Sci (2008): 4506

Trans-scleral permeability of Oregon green 488
Authors: Lee SJ, Kim SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: J Ocul Pharmacol Ther (2008): 579

A mismatch-selective bifunctional rhodium-Oregon Green conjugate: a fluorescent probe for mismatched DNA
Authors: Zeglis BM, Barton JK.
Journal: J Am Chem Soc (2006): 5654

The Pennsylvania Green Fluorophore: a hybrid of Oregon Green and Tokyo Green for the construction of hydrophobic and pH-insensitive molecular probes
Authors: Mottram LF, Boonyarattanakalin S, Kovel RE, Peterson BR.
Journal: Org Lett (2006): 581

Cetuximab-Oregon Green 488
Authors: Unknown
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD) (2004)

Collagen-binding adhesion protein 35-Oregon Green 488
Authors: Cheng KT, Van Zandvoort M.
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)

Alexa and Oregon Green dyes as fluorescence anisotropy probes for measuring protein-protein and protein-nucleic acid interactions
Authors: Rusinova E, Tretyachenko-Ladokhina V, Vele OE, Senear DF, Alexander Ross JB.
Journal: Anal Biochem (2002): 18

Imaging of changes in sarcoplasmic reticulum [Ca(2+)] using Oregon Green BAPTA 5N and confocal laser scanning microscopy
Authors: White C, McGeown G.
Journal: Cell Calcium (2002): 151

说明书
5-OG488 酸(相当于Oregon Green 488 羧酸,5-异构体).pdf

5-OG488,SE(相当于Oregon Green 488 SE,5-异构体) 货号710-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

5-OG488,SE(相当于Oregon Green 488 SE,5-异构体)

5-OG488,SE(相当于Oregon Green 488 SE,5-异构体)

5-OG488,SE(相当于Oregon Green 488 SE,5-异构体)    货号710 货号 710 存储条件 在零下15度以下保存, 避免光照
规格 5 mg 价格 2604
Ex (nm) 498 Em (nm) 526
分子量 509.37 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:710

产品名称:5-OG488,SE

规格:5mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:509.37

溶剂:DMSO

激发波长(nm):498

发射波长(nm):526

 

产品介绍

5-OG488,SE是美国AAT Bioquest生产的荧光染料。5-OG488琥珀酰亚胺酯与OregonGreen®488羧酸,琥珀酰亚胺酯,5-异构体(Invitrogen#O6147,OregonGreen®是Invitrogen的商标)是相同的分子。这种化合物具有较高的光稳定性和较低的pKa~4.7,而5-FAM具有pKa~6.4。5-OG488的pKa降低使其荧光在生理pH范围内基本上对pH不敏感。该胺反应性5-OG488琥珀酰亚胺酯可用于制备亮绿色荧光生物缀合物,其激发/发射最大值为~496 / 524nm。它是5-FITC的极好替代品。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的5-OG488,SE。 

点击查看光谱

 

参考文献

Biodegradable polymersomes as carriers and release systems for paclitaxel using Oregon Green(R) 488 labeled paclitaxel as a model compound
Authors: Lee JS, Feijen J.
Journal: J Control Release (2012): 312

Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377

Pharmacokinetics of intraocular drug delivery of Oregon green 488-labeled triamcinolone by subtenon injection using ocular fluorophotometry in rabbit eyes
Authors: Lee SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: Invest Ophthalmol Vis Sci (2008): 4506

Trans-scleral permeability of Oregon green 488
Authors: Lee SJ, Kim SJ, Kim ES, Geroski DH, McCarey BE, Edelhauser HF.
Journal: J Ocul Pharmacol Ther (2008): 579

A mismatch-selective bifunctional rhodium-Oregon Green conjugate: a fluorescent probe for mismatched DNA
Authors: Zeglis BM, Barton JK.
Journal: J Am Chem Soc (2006): 5654

The Pennsylvania Green Fluorophore: a hybrid of Oregon Green and Tokyo Green for the construction of hydrophobic and pH-insensitive molecular probes
Authors: Mottram LF, Boonyarattanakalin S, Kovel RE, Peterson BR.
Journal: Org Lett (2006): 581

Cetuximab-Oregon Green 488
Authors: Unknown
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD) (2004)

Collagen-binding adhesion protein 35-Oregon Green 488
Authors: Cheng KT, Van Zandvoort M.
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)

Alexa and Oregon Green dyes as fluorescence anisotropy probes for measuring protein-protein and protein-nucleic acid interactions
Authors: Rusinova E, Tretyachenko-Ladokhina V, Vele OE, Senear DF, Alexander Ross JB.
Journal: Anal Biochem (2002): 18

Imaging of changes in sarcoplasmic reticulum [Ca(2+)] using Oregon Green BAPTA 5N and confocal laser scanning microscopy
Authors: White C, McGeown G.
Journal: Cell Calcium (2002): 151

 

相关产品

产品名称 货号
6-OG488,SE Cat#711

说明书
5-OG488,SE(相当于Oregon Green 488 SE,5-异构体).pdf

钙离子荧光探针Oregon Green 488 BAPTA-1,AM 货号20507-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

钙离子荧光探针Oregon Green 488 BAPTA-1,AM

钙离子荧光探针Oregon Green 488 BAPTA-1,AM

钙离子荧光探针Oregon Green 488 BAPTA-1,AM    货号20507 货号 20507 存储条件 在零下15度以下保存, 避免光照
规格 10×50 ug 价格 2604
Ex (nm) 493 Em (nm) 522
分子量 1258.07 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:20507

产品名称:钙离子荧光探针Oregon Green 488 BAPTA-1,AM

规格:10×50 ug

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:1258.07

溶剂:DMSO

激发波长(nm):494

发射波长(nm):523

 

适用仪器


流式细胞仪  
激发: 488nm激光
发射: 530/30nm滤波片
通道: FITC通道
荧光显微镜  
激发: FITC
发射: FITC
推荐孔板: 黑色透明
荧光酶标仪  
激发: 490nm
发射: 525nm
cutoff: 515nm
推荐孔板: 黑色透明
读取模式: 底读模式

 

产品介绍

OG488 BAPTA -1 AM 与 Oregon Green 488 BAPTA-1 AM 酯的分子相同。它是一种细胞渗透性和可见光可激发的钙指示剂,通常与 FITC 滤光片组一起使用。通过将溶解的指示剂直接添加到含有培养细胞的培养皿中,可以为细胞加载 OG488 BAPTA -1 AM。来自这些细胞的荧光信号通常使用荧光显微镜、荧光微孔板测定或流式细胞术测量。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的钙离子荧光探针Oregon Green 488 BAPTA-1,AM。 

点击查看光谱

点击查看实验方案

钙离子篇:时间轴式讲解应用于钙离子检测的探针

 

参考文献

Norepinephrine-induced calcium signaling in astrocytes in the respiratory network of the ventrolateral medulla
Authors: Schnell C, Negm M, Driehaus J, Scheller A, Hulsmann S.
Journal: Respir Physiol Neurobiol (2016): 18

Optical electrocorticogram (OECoG) using wide-field calcium imaging reveals the divergence of neuronal and glial activity during acute rodent seizures
Authors: Daniel AG, Laffont P, Zhao M, Ma H, Schwartz TH.
Journal: Epilepsy Behav (2015): 61

A highly sensitive fluorescent indicator dye for calcium imaging of neural activity in vitro and in vivo
Authors: Tada M, Takeuchi A, Hashizume M, Kitamura K, Kano M.
Journal: Eur J Neurosci (2014): 1720

Two-Photon Processor and SeNeCA: a freely available software package to process data from two-photon calcium imaging at speeds down to several milliseconds per frame
Authors: Tomek J, Novak O, Syka J.
Journal: J Neurophysiol (2013): 243

Calcium buffering and clearance in spider mechanosensory neurons
Authors: Schmitz J, Hoger U, Torkkeli PH, French AS.
Journal: J Comp Physiol A Neuroethol Sens Neural Behav Physiol (2012): 477

Post hoc immunostaining of GABAergic neuronal subtypes following in vivo two-photon calcium imaging in mouse neocortex
Authors: Langer D, Helmchen F.
Journal: Pflugers Arch (2012): 339

Astrocyte calcium signaling transforms cholinergic modulation to cortical plasticity in vivo
Authors: Takata N, Mishima T, Hisatsune C, Nagai T, Ebisui E, Mikoshiba K, Hirase H.
Journal: J Neurosci (2011): 18155

Microscopic imaging of intracellular calcium in live cells using lifetime-based ratiometric measurements of Oregon Green BAPTA-1
Authors: Lattarulo C, Thyssen D, Kuchibholta KV, Hyman BT, Bacskaiq BJ.
Journal: Methods Mol Biol (2011): 377

Multifocal animated imaging of changes in cellular oxygen and calcium concentrations and membrane potential within the intact adult mouse carotid body ex vivo
Authors: Wotzlaw C, Bernardini A, Berchner-Pfannschmidt U, Papkovsky D, Acker H, Fandrey J.
Journal: Am J Physiol Cell Physiol (2011): C266

Somatic depolarization enhances GABA release in cerebellar interneurons via a calcium/protein kinase C pathway
Authors: Bouhours B, Trigo FF, Marty A.
Journal: J Neurosci (2011): 5804

说明书
钙离子荧光探针Oregon Green 488 BAPTA-1,AM.pdf