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FIASH试剂
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货号 | 22330 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 100 Tests | 价格 | 4836 | |
| Ex (nm) | 498 | Em (nm) | 517 | |
| 分子量 | 664.49 | 溶剂 | DMSO | |
| 产品详细介绍 | ||||
简要概述
产品基本信息
货号：22330
产品名称：FIASH试剂
规格：100 Tests
储存条件：-20℃干燥
保质期：12个月
产品物理化学光谱特性
外观：固体
分子量：664.49
溶剂：DMSO
Ex(nm):498
Em(nm):517
适用仪器
| 荧光显微镜 | |
| 激发： | FITC滤波片组 |
| 发射： | FITC滤波片组 |
| 推荐孔板： | 黑色透明 |
产品介绍
FlASH是一种荧光素衍生物，被修饰为包含两个彼此相距一定距离的砷原子。它由Roger Tsien及其同事在1998年开发。双砷标记技术通过砷与硫醇的高亲和力相互作用起作用。当FlASH结合四半胱氨酸（TC）序列时，其双砷基与Cys-Cys部分快速反应，并且标签变为绿色的高度荧光。双砷标记试剂FIASH是用于标记包含具有Cys-Cys-X1-X2-Cys-Cys氨基酸序列的六氨基酸基序的蛋白质的最小表达标签。最常用的四半胱氨酸是六个氨基酸的Cys-Cys-Pro-Gly-Cys-Cys序列。由于此序列很少出现在内源蛋白中，将序列整合到靶蛋白中会产生一个小的但高度特异性的蛋白标记靶。当与包含四半胱氨酸基序Cys-Cys-Pro-Gly-Cys-Cys的重组蛋白结合时，FIASH会产生强烈的绿色荧光信号。它可以用于检测蛋白质的定位，周转和运输，受体信号传导和内在化。
参考文献
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