上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
Cell Meter 比色法MTT细胞增殖检测试剂盒
| 货号 | 22768 | 存储条件 | Multiple |
| 规格 | 1000 Tests | 价格 | 3732 |
| Ex (nm) | Em (nm) | ||
| 分子量 | 溶剂 | ||
| 产品详细介绍 | |||
简要概述
Cell Meter 检测试剂盒是一套用于检测细胞活力的工具。 Cell Meter 比色法MTT细胞增殖检测试剂盒使用MTT（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴化物）定量活细胞的数量。水溶性MTT在代谢活性细胞中产生水不溶性紫色甲瓒。产生的甲瓒的数量与活细胞的数量成正比。在560 nm附近测得吸光度增加。与其他需要繁琐的增溶步骤来溶解不溶于水的甲瓒产物的其他商业MTT测定法不同，Cell Meter 比色MTT细胞增殖试剂盒只需极少的人工操作。我们专有的配方消除了费时的增溶步骤。它是确定细胞增殖和细胞毒性测定中活细胞数量的最强大的MTT试验。检测灵敏度比传统的MTT分析高10倍。
产品说明书
样品分析方案
概述
在96孔板中制备细胞（100 µL /孔）
添加MTT工作溶液（140 µL /孔）
在37°C下孵育2-4小时
读取560 nm处的吸光度
注：使用前，请在室温下解冻所有试剂盒组分。
工作溶液制备：
通过将4 mL MTT试剂A（组分A）与10 mL MTT试剂B（组分B）（2：5，MTT试剂A：B的体积比）混合，制备所需的MTT工作溶液量，混匀。
注意：14 mL MTT工作溶液足以在96孔板上进行100次测试。实验前准备足够的MTT工作溶液，并及时使用。
操作步骤
1.在具有透明底的组织培养孔板中，培养1000至40,000个细胞/孔。
2.将测试化合物添加到细胞中，并在37°C，5％CO2培养箱中孵育所需的时间（例如24、48或96小时）。3.对于空白孔（不含细胞的培养基），添加相同量的测试化合物。对于96孔板，建议的总体积为100 µL，对于384孔板，建议的总体积为25 µL。
注意：应对每个细胞系进行单独评估，以确定增殖或诱导细胞毒性的最佳细胞密度。对于增殖测定，使用较少的细胞；对于细胞毒性测定，请使用更多的细胞作为开始。
4.向每个孔中加入140 µL /孔（96孔板）或35 µL /孔（384孔板）。
5.在37°C下孵育平板2-4小时，避光。
注意：根据不同的细胞类型和使用的细胞浓度，孵育时间可能从2小时到过夜。优化每个实验的孵育时间。
用吸光度板读数器在OD = 562 nm处检测吸光度。
图示
图1.用Cell Meter 比色法MTT细胞增殖试剂盒确定细胞数。将0至40,000个细胞/孔/ 100 µL的HeLa细胞添加到透明的底部96孔板中过夜。使用SpectraMax读数器（Molecular Devices）在560nm处检测吸光度。
参考文献
Novel Dental Poly (Methyl Methacrylate) Containing Phytoncide for Antifungal Effect and Inhibition of Oral Multispecies Biofilm.
Authors: Lee, Myung-Jin and Kim, Min-Ji and Oh, Sang-Hwan and Kwon, Jae-Sung
Journal: Materials (Basel, Switzerland) (2020)
Toxicity of diuron metabolites in human cells.
Authors: Mohammed, Ali Mustafa and Huovinen, Marjo and Vähäkangas, Kirsi H
Journal: Environmental toxicology and pharmacology (2020): 103409
Biological Effect of Modern Fetal Ultrasound Techniques on Human Dermal Fibroblast Cells.
Authors: M, Morshedi and M, Bakhshandeh and A, Piryaei and A, Emami and M, Zangeneh and A, Razzaghdoust and H, Ghadiri and F, Zayeri
Journal: Journal of biomedical physics & engineering (2019): 335-344
Development and validation of UPLC method for WST-1 cell viability assay and its application to MCTT HCE™ eye irritation test for colorful substances.
Authors: Joo, Kyung-Mi and Kim, Seolyeong and Koo, Ye Ji and Lee, Miri and Lee, Su-Hyun and Choi, Dalwoong and Lim, Kyung-Min
Journal: Toxicology in vitro : an international journal published in association with BIBRA (2019): 412-419
Effects Of Adenosine On Apoptosis Of Ovarian Cancer A2780 Cells Via ROS And Caspase Pathways.
Authors: Xia, Bing and Wang, Jing
Journal: OncoTargets and therapy (2019): 9473-9480
Evaluation of hepatotoxicity potential of a potent traditional Tibetan medicine Zuotai.
Authors: Zhou, Liang-Liang and Chen, Hai-Juan and He, Qiang-Qiang and Li, Cen and Wei, Li-Xin and Shang, Jing
Journal: Journal of ethnopharmacology (2019): 112-118
[Comparative study on quality of decoction pieces of Saposhnikovia divaricata with different growth patterns and years and thinking of standard of decoction pieces of S. divaricata in Chinese Pharmacopoeia].
Authors: Xue, Xue and Wang, Hao and Jia, Tian-Ying and Qu, Wen-Jia and Wang, Hai-Li and Xin, Jie-Ping and Liu, Meng-Nan and Xiong, Hui and Li, Xiang-Ri
Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (2019): 4034-4042
Colorimetric method for determining viability of sea urchin sperm applied in toxicity tests.
Authors: Resgalla, Charrid and Máximo, Marcus Vinícius and Brasil, Mirella do Nascimento and Pessatti, Marcos Luiz
Journal: Ecotoxicology (London, England) (2018): 499-504
Development of extremely stable dual functionalized gold nanoparticles for effective colorimetric detection of clenbuterol and ractopamine in human urine samples.
Authors: Simon, Turibius and Shellaiah, Muthaiah and Steffi, Perpectual and Sun, Kien Wen and Ko, Fu-Hsiang
Journal: Analytica chimica acta (2018): 96-104
New platinum (II) and palladium (II) complexes of coumarin-thiazole Schiff base with a fluorescent chemosensor properties: Synthesis, spectroscopic characterization, X-ray structure determination, in vitro anticancer activity on various human carcinoma cell lines and computational studies.
Authors: Şahin, Ömer and Özdemir, Ümmühan Özmen and Seferoğlu, Nurgül and Genc, Zuhal Karagöz and Kaya, Kerem and Aydıner, Burcu and Tekin, Suat and Seferoğlu, Zeynel
Journal: Journal of photochemistry and photobiology. B, Biology (2018): 428-439
