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Cell Meter VX450固定化细胞活性染料
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货号 | 22540 | 存储条件 | 在零下15度以下保存, 避免光照 |
规格 | 200 Tests | 价格 | 2472 | |
Ex (nm) | 406 | Em (nm) | 445 | |
分子量 | 溶剂 | DMSO | ||
产品详细介绍 |
简要概述
产品基本信息
货号:22540
产品名称:Cell Meter VX450固定化细胞活性染料
规格:200 Tests
储存条件:保存在冰箱-15℃干燥
保质期:12个月
产品物理化学光谱特性
分子量:N/A
激发波长(nm):406
发射波长(nm):445
适用仪器
流式细胞仪 | |
激发: | 405nm激光 |
发射: | 525/40nm滤波片 |
通道: | AmCyan通道 |
荧光显微镜 | |
激发: | 紫色滤波片组 |
发射: | 紫色滤波片组 |
推荐孔板: | 黑色透明 |
产品介绍
从活细胞中区分和排除死细胞可以更清晰地分离和鉴定细胞群。Cell Meter VX450固定化细胞活性染料是一类细胞不可渗透的荧光活性染料,它们经过优化以匹配常见流式细胞仪的主要激发激光,例如350、405、488、633和647 nm。这些染料不渗入活细胞,但渗入膜受损的细胞。它们与含胺和硫醇的蛋白质以及其他细胞成分发生不可逆的反应。由于具有受损膜的死细胞或固定细胞更容易与Cell Meter VX450固定化细胞活性染料发生反应,因此比具有完整膜的活细胞的染色更亮,因此这些染料可用于评估哺乳动物细胞的活与死状态。使用这些染料时,需要考虑一些因素,例如,每种染料的滴定,以确保活细胞几乎没有染色。Cell Meter VX450固定化细胞活性染料经过优化,可以在405 nm的紫色激光激发下以450 nm的波长发射。与其他商业上类似的活性染料相比,这种固定化细胞活性染料更加耐用、稳定。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Cell Meter VX450固定化细胞活性染料。
点击查看光谱
图示
![]() 图1.通过Cell Meter 固定化活性染料检测Jurkat细胞活性。处理Jurkat细胞并用Cell Meter VX450(Cat#22540)染色,然后固定在3.7%甲醛中并通过流式细胞仪进行分析。通过AmCyan通道可以很容易地将死细胞群体(蓝色峰)与活细胞群体(红色峰)区分开,在固定之前和之后都获得了几乎相同的结果。 |
参考文献
CFSE dilution to study human T and NK cell proliferation in vitro.
Authors: Terrén, Iñigo and Orrantia, Ane and Vitallé, Joana and Zenarruzabeitia, Olatz and Borrego, Francisco
Journal: Methods in enzymology (2020): 239-255
PGC-1α Regulates Cell Proliferation and Invasion via AKT/GSK-3β/β-catenin Pathway in Human Colorectal Cancer SW620 and SW480 Cells.
Authors: Yun, Seong-Hoon and Park, Joo-In
Journal: Anticancer research (2020): 653-664
The miR-3940-5p inhibits cell proliferation of gingival mesenchymal stem cells.
Authors: Han, Xiao and Yang, Haoqing and Cao, Yangyang and Ge, Lihua and Han, Nannan and Zhang, Chen and Fan, Zhipeng and Yao, Rui
Journal: Oral diseases (2019): 1363-1373
Evaluation of Cell Proliferation and Apoptosis in Immunotoxicity Testing.
Authors: Nagarkatti, Mitzi and Rieder, Sadiye Amcaoglu and Nagarkatti, Prakash S
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 209-230
Toll-Like Receptor 4 (TLR4)/Cyclooxygenase-2 (COX-2) Regulates Prostate Cancer Cell Proliferation, Migration, and Invasion by NF-κB Activation.
Authors: Wang, Wei and Wang, Jiye
Journal: Medical science monitor : international medical journal of experimental and clinical research (2018): 5588-5597
Gag-Specific CD4 T Cell Proliferation, Plasmacytoid Dendritic Cells, and Ethnicity in Perinatally HIV-1-Infected Youths: The ANRS-EP38-IMMIP Study.
Authors: Scott-Algara, Daniel and Warszawski, Josiane and Chenadec, Jérôme Le and Didier, Céline and Montange, Thomas and Viard, Jean-Paul and Dollfus, Catherine and Avettand-Fenoel, Véronique and Rouzioux, Christine and Blanche, Stéphane and Buseyne, Florence
Journal: AIDS research and human retroviruses (2017): 21-28
Quantitative analysis of cell proliferation by a dye dilution assay: Application to cell lines and cocultures.
Authors: Chung, Soobin and Kim, Seol-Hee and Seo, Yuri and Kim, Sook-Kyung and Lee, Ji Youn
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2017): 704-712
[Myeloid-derived suppressor cells promoted autologous B cell proliferation in rheumatoid arthritis].
Authors: Chen, W and Hu, F L and Liu, H J and Xu, L L and Li, Y N and Li, Z G
Journal: Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences (2017): 819-823
A comparative study of colorimetric cell proliferation assays in immune cells.
Authors: Koyanagi, Madoka and Kawakabe, So and Arimura, Yutaka
Journal: Cytotechnology (2016): 1489-98
Hypoxia inhibits mesenchymal stem cell proliferation through HIF1α-dependent regulation of P27.
Authors: Kumar, Sanjay and Vaidya, Meenal
Journal: Molecular and cellular biochemistry (2016): 29-38