TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*

简要概述

产品基本信息

货号：17287

产品名称：TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*

规格：5ml

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

溶剂：水

产品介绍

用于 TaqMan 探针的 TAQuest qPCR Master Mix 是一种即用型 2X溶液，针对 qPCR 和与 TaqMan 基因表达分析兼容的两步法 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中提供了所有基本成分，包括我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP，但模板、引物和探针除外。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应，从而最大限度地减少非特异性产物的形成。优化的成分可确保 PCR 对所有样本类型（如基因组、质粒、病毒和 cDNA 模板）的特异性和灵敏度。用于 TaqMan 探针的 TAQuest qPCR Master Mix 设计用于使用内部阳性对照的双链反应。该预混液含大量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*。 

适用仪器

样品实验方案
注意 在室温下解冻TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

表 1. 各反应每孔试剂组成

表 2. 热循环参数

参考文献

Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.
Authors: Lista, Maria Jose and Matos, Pedro M and Maguire, Thomas J A and Poulton, Kate and Ortiz-Zapater, Elena and Page, Robert and Sertkaya, Helin and Ortega-Prieto, Ana M and Scourfield, Edward and O’Byrne, Aoife M and Bouton, Clement and Dickenson, Ruth E and Ficarelli, Mattia and Jimenez-Guardeño, Jose M and Howard, Mark and Betancor, Gilberto and Galao, Rui Pedro and Pickering, Suzanne and Signell, Adrian W and Wilson, Harry and Cliff, Penelope and Kia Ik, Mark Tan and Patel, Amita and MacMahon, Eithne and Cunningham, Emma and Doores, Katie and Agromayor, Monica and Martin-Serrano, Juan and Perucha, Esperanza and Mischo, Hannah E and Shankar-Hari, Manu and Batra, Rahul and Edgeworth, Jonathan and Zuckerman, Mark and Malim, Michael H and Neil, Stuart and Martinez-Nunez, Rocio Teresa
Journal: PloS one (2021): e0256813

Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.
Authors: Xu, Xingang and Yang, Feng and Zhang, Qi and Xu, Ying and Huang, Jiali and Fu, Mingzhe and Zhang, Weimin
Journal: Journal of virological methods (2019): 58-64

Evaluation and utilization of preassembled frozen commercial fast real-time qPCR master mixes for detection of cytomegalovirus and BK virus.
Authors: Glover, William A and Atienza, Ederlyn E and Nesbitt, Shannon and Kim, Woo J and Castor, Jared and Cook, Linda and Jerome, Keith R
Journal: Journal of medical virology (2016): 115-9

Frequency-encoded laser-induced fluorescence for multiplexed detection in infrared-mediated quantitative PCR.
Authors: Schrell, Adrian M and Roper, Michael G
Journal: The Analyst (2014): 2695-701

Real-time stability testing of air-dried primers and fluorogenic hydrolysis probes stabilized by trehalose and xanthan.
Authors: Rombach, Markus and Kosse, Dominique and Faltin, Bernd and Wadle, Simon and Roth, Günter and Zengerle, Roland and von Stetten, Felix
Journal: BioTechniques (2014): 151-5

Development of a novel internal positive control for Taqman based assays.
Authors: Hartman, Laurie J and Coyne, Susan R and Norwood, David A
Journal: Molecular and cellular probes (2005): 51-9

[Establishment and application of real-time fluorescence polymerase chain reaction based on the TaqMan probes for detection of Yersinia pestis].
Authors: Li, Wei and Hai, Rong and Yu, Dong-zheng and Zhang, Zhi-kai and Cai, Hong
Journal: Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi (2005): 613-6

[Multiplex PCR for detection and quantification of GM potato event EH92-527-1 in food].
Authors: Tyshko, N V and Sadykova, E O and Grouzdev, D S and Sukhacheva, M V
Journal: Voprosy pitaniia: 57-61

[Multiplex polymerase chain reaction for genetically modified potato event AV43-6-G7 quantification. Proof of efficiency].
Authors: Tyshko, N V and Sadykova, E O and Sukhacheva, M V and Grouzdev, D S
Journal: Voprosy pitaniia: 62-70

TAQuest qPCR Master Mix 用于TaqMan探针*无ROX*

	货号	17282	存储条件	在零下15度以下保存, 避免光照
	规格	1 mL	价格	1164
	Ex (nm)		Em (nm)
	分子量		溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：17282

产品名称：TAQuest qPCR Master Mix 用于TaqMan探针*无ROX*

规格：1ml

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

溶剂：水

产品介绍

用于 TaqMan 探针的 TAQuest qPCR Master Mix 是一种即用型 2X溶液，针对 qPCR 和与 TaqMan 基因表达分析兼容的两步法 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中提供了所有基本成分，包括我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP，但模板、引物和探针除外。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应，从而最大限度地减少非特异性产物的形成。优化的成分可确保 PCR 对所有样本类型（如基因组、质粒、病毒和 cDNA 模板）的特异性和灵敏度。用于 TaqMan 探针的 TAQuest qPCR Master Mix 设计用于使用内部阳性对照的双链反应。该预混液不含 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest qPCR Master Mix 用于TaqMan探针*无ROX*。 

适用仪器

样品实验方案
注意 在室温下解冻TAQuest qPCR Master Mix 用于TaqMan探针*无ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

表 1. 各反应每孔试剂组成

表 2. 热循环参数

参考文献

Evaluation of SYBR Green real time PCR for detecting SARS-CoV-2 from clinical samples.
Authors: Pereira-Gómez, Marianoel and Fajardo, Álvaro and Echeverría, Natalia and López-Tort, Fernando and Perbolianachis, Paula and Costábile, Alicia and Aldunate, Fabián and Moreno, Pilar and Moratorio, Gonzalo
Journal: Journal of virological methods (2021): 114035

Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR.
Authors: Dorlass, Erick Gustavo and Monteiro, Cairo Oliveira and Viana, Amanda Oliveira and Soares, Camila Pereira and Machado, Rafael Rahal Guaragna and Thomazelli, Luciano Matsumiya and Araujo, Danielle Bastos and Leal, Fabyano Bruno and Candido, Erika Donizette and Telezynski, Bruna Larotonda and Valério, Camila Araujo and Chalup, Vanessa Nascimento and Mello, Ralyria and Almeida, Flavia Jaqueline and Aguiar, Andressa Simões and Barrientos, Anna Carlotta Mott and Sucupira, Carolina and De Paulis, Milena and Sáfadi, Marco Aurélio Palazzi and Silva, Daniella Gregorio Bonfim Prado and Sodré, Janaina Joice Martins and Soledade, Mariana Pereira and Matos, Samantha Faria and Ferreira, Sabrina Rodrigues and Pinez, Célia Miranda Nunez and Buonafine, Carolina Palamin and Pieroni, Leticia Nery Ferreira and Malta, Fernanda Mello and Santana, Rubia Anita Ferraz and Souza, Eloisa Corrêa and Fock, Ricardo Ambrosio and Pinho, João Renato Rebelo and Ferreira, Luís Carlos Souza and Botosso, Viviane Fongaro and Durigon, Edison Luiz and Oliveira, Danielle Bruna Leal
Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2020): 1117-1123

Multiplex SYBR Green and duplex TaqMan real-time PCR assays for the detection of Photorhabdus Insect-Related (Pir) toxin genes pirA and pirB.
Authors: Cruz-Flores, Roberto and Mai, Hung Nam and Dhar, Arun K
Journal: Molecular and cellular probes (2019): 20-28

Quantification of M13 and T7 bacteriophages by TaqMan and SYBR green qPCR.
Authors: Peng, Xiujuan and Nguyen, Alex and Ghosh, Debadyuti
Journal: Journal of virological methods (2018): 100-107

sjTREC quantification using SYBR quantitative PCR for age estimation of bloodstains in a Japanese population.
Authors: Yamanoi, Eisuke and Uchiyama, Saori and Sakurada, Makoto and Ueno, Yasuhiro
Journal: Legal medicine (Tokyo, Japan) (2018): 71-74

Comparison and evaluation of conventional RT-PCR, SYBR green I and TaqMan real-time RT-PCR assays for the detection of porcine epidemic diarrhea virus.
Authors: Zhou, Xinrong and Zhang, Tiansheng and Song, Deping and Huang, Tao and Peng, Qi and Chen, Yanjun and Li, Anqi and Zhang, Fanfan and Wu, Qiong and Ye, Yu and Tang, Yuxin
Journal: Molecular and cellular probes (2017): 36-41

Field Validation of SYBR Green- and TaqMan-Based Real-Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic.
Authors: Gomes, Ciro Martins and Cesetti, Mariana Vicente and de Paula, Natália Aparecida and Vernal, Sebastián and Gupta, Gaurav and Sampaio, Raimunda Nonata Ribeiro and Roselino, Ana Maria
Journal: Journal of clinical microbiology (2017): 526-534

Qualitative Sybr Green real-time detection of single nucleotide polymorphisms responsible for target-site resistance in insect pests: the example of Myzus persicae and Musca domestica.
Authors: Puggioni, V and Chiesa, O and Panini, M and Mazzoni, E
Journal: Bulletin of entomological research (2017): 96-105

Sybr Green- and TaqMan-Based Quantitative PCR Approaches Allow Assessment of the Abundance and Relative Distribution of Frankia Clusters in Soils.
Authors: Ben Tekaya, Seifeddine and Ganesan, Abirama Sundari and Guerra, Trina and Dawson, Jeffrey O and Forstner, Michael R J and Hahn, Dittmar
Journal: Applied and environmental microbiology (2017)

An Evaluation of Quantitative PCR Assays (TaqMan® and SYBR Green) for the Detection of Babesia bigemina and Babesia bovis, and a Novel Fluorescent-ITS1-PCR Capillary Electrophoresis Method for Genotyping B. bovis Isolates.
Authors: Zhang, Bing and Sambono, Jacqueline L and Morgan, Jess A T and Venus, Bronwyn and Rolls, Peter and Lew-Tabor, Ala E
Journal: Veterinary sciences (2016)

TAQuest qPCR Master Mix 用于TaqMan探针*无ROX*

	货号	17282	存储条件	在零下15度以下保存, 避免光照
	规格	1 mL	价格	1164
	Ex (nm)		Em (nm)
	分子量		溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：17282

产品名称：TAQuest qPCR Master Mix 用于TaqMan探针*无ROX*

规格：1ml

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

溶剂：水

产品介绍

用于 TaqMan 探针的 TAQuest qPCR Master Mix 是一种即用型 2X溶液，针对 qPCR 和与 TaqMan 基因表达分析兼容的两步法 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中提供了所有基本成分，包括我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP，但模板、引物和探针除外。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应，从而最大限度地减少非特异性产物的形成。优化的成分可确保 PCR 对所有样本类型（如基因组、质粒、病毒和 cDNA 模板）的特异性和灵敏度。用于 TaqMan 探针的 TAQuest qPCR Master Mix 设计用于使用内部阳性对照的双链反应。该预混液不含 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest qPCR Master Mix 用于TaqMan探针*无ROX*。 

适用仪器

样品实验方案
注意 在室温下解冻TAQuest qPCR Master Mix 用于TaqMan探针*无ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

表 1. 各反应每孔试剂组成

表 2. 热循环参数

参考文献

Evaluation of SYBR Green real time PCR for detecting SARS-CoV-2 from clinical samples.
Authors: Pereira-Gómez, Marianoel and Fajardo, Álvaro and Echeverría, Natalia and López-Tort, Fernando and Perbolianachis, Paula and Costábile, Alicia and Aldunate, Fabián and Moreno, Pilar and Moratorio, Gonzalo
Journal: Journal of virological methods (2021): 114035

Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR.
Authors: Dorlass, Erick Gustavo and Monteiro, Cairo Oliveira and Viana, Amanda Oliveira and Soares, Camila Pereira and Machado, Rafael Rahal Guaragna and Thomazelli, Luciano Matsumiya and Araujo, Danielle Bastos and Leal, Fabyano Bruno and Candido, Erika Donizette and Telezynski, Bruna Larotonda and Valério, Camila Araujo and Chalup, Vanessa Nascimento and Mello, Ralyria and Almeida, Flavia Jaqueline and Aguiar, Andressa Simões and Barrientos, Anna Carlotta Mott and Sucupira, Carolina and De Paulis, Milena and Sáfadi, Marco Aurélio Palazzi and Silva, Daniella Gregorio Bonfim Prado and Sodré, Janaina Joice Martins and Soledade, Mariana Pereira and Matos, Samantha Faria and Ferreira, Sabrina Rodrigues and Pinez, Célia Miranda Nunez and Buonafine, Carolina Palamin and Pieroni, Leticia Nery Ferreira and Malta, Fernanda Mello and Santana, Rubia Anita Ferraz and Souza, Eloisa Corrêa and Fock, Ricardo Ambrosio and Pinho, João Renato Rebelo and Ferreira, Luís Carlos Souza and Botosso, Viviane Fongaro and Durigon, Edison Luiz and Oliveira, Danielle Bruna Leal
Journal: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] (2020): 1117-1123

Multiplex SYBR Green and duplex TaqMan real-time PCR assays for the detection of Photorhabdus Insect-Related (Pir) toxin genes pirA and pirB.
Authors: Cruz-Flores, Roberto and Mai, Hung Nam and Dhar, Arun K
Journal: Molecular and cellular probes (2019): 20-28

Quantification of M13 and T7 bacteriophages by TaqMan and SYBR green qPCR.
Authors: Peng, Xiujuan and Nguyen, Alex and Ghosh, Debadyuti
Journal: Journal of virological methods (2018): 100-107

sjTREC quantification using SYBR quantitative PCR for age estimation of bloodstains in a Japanese population.
Authors: Yamanoi, Eisuke and Uchiyama, Saori and Sakurada, Makoto and Ueno, Yasuhiro
Journal: Legal medicine (Tokyo, Japan) (2018): 71-74

Comparison and evaluation of conventional RT-PCR, SYBR green I and TaqMan real-time RT-PCR assays for the detection of porcine epidemic diarrhea virus.
Authors: Zhou, Xinrong and Zhang, Tiansheng and Song, Deping and Huang, Tao and Peng, Qi and Chen, Yanjun and Li, Anqi and Zhang, Fanfan and Wu, Qiong and Ye, Yu and Tang, Yuxin
Journal: Molecular and cellular probes (2017): 36-41

Field Validation of SYBR Green- and TaqMan-Based Real-Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic.
Authors: Gomes, Ciro Martins and Cesetti, Mariana Vicente and de Paula, Natália Aparecida and Vernal, Sebastián and Gupta, Gaurav and Sampaio, Raimunda Nonata Ribeiro and Roselino, Ana Maria
Journal: Journal of clinical microbiology (2017): 526-534

Qualitative Sybr Green real-time detection of single nucleotide polymorphisms responsible for target-site resistance in insect pests: the example of Myzus persicae and Musca domestica.
Authors: Puggioni, V and Chiesa, O and Panini, M and Mazzoni, E
Journal: Bulletin of entomological research (2017): 96-105

Sybr Green- and TaqMan-Based Quantitative PCR Approaches Allow Assessment of the Abundance and Relative Distribution of Frankia Clusters in Soils.
Authors: Ben Tekaya, Seifeddine and Ganesan, Abirama Sundari and Guerra, Trina and Dawson, Jeffrey O and Forstner, Michael R J and Hahn, Dittmar
Journal: Applied and environmental microbiology (2017)

An Evaluation of Quantitative PCR Assays (TaqMan® and SYBR Green) for the Detection of Babesia bigemina and Babesia bovis, and a Novel Fluorescent-ITS1-PCR Capillary Electrophoresis Method for Genotyping B. bovis Isolates.
Authors: Zhang, Bing and Sambono, Jacqueline L and Morgan, Jess A T and Venus, Bronwyn and Rolls, Peter and Lew-Tabor, Ala E
Journal: Veterinary sciences (2016)

TAQuest qPCR Master Mix with Helixyte Green *高ROX*

简要概述

产品基本信息

货号：17275

产品名称：TAQuest qPCR Master Mix with Helixyte Green *高ROX*

规格：5ml

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

溶剂：水

产品介绍

TAQuest qPCR Master Mix with Helixyte Green 是一种即用型 2X溶液，针对 qPCR 和 2 步 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中包含我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP。您只需添加模板和目标引物即可运行所需的 PCR 反应。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应，从而最大限度地减少非特异性产物的形成。该酶与优化的缓冲液结合使用，可确保对所有样品类型（如基因组、质粒、病毒和 cDNA 模板）的 PCR 特异性和灵敏度。 Helixyte Green 嵌入染料无需使用序列特异性探针即可快速检测和分析DNA。该预混液包含大量ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest qPCR Master Mix with Helixyte Green *高ROX*。 

适用仪器

样品实验方案
注意 在室温下用 Helixyte Green *高ROX* 解冻 TAQuest™ qPCR Master Mix。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

表 1. 各反应每孔试剂组成

表 2. 热循环参数

参考文献

Aligned Expression of IFI16 and STING Genes in RRMS Patients’ Blood.
Authors: Helbi, Sobhan and Ravanbakhsh, Behnam and Karimi, Mohammad and Kooti, Wesam and Jivad, Nahid
Journal: Endocrine, metabolic & immune disorders drug targets (2020): 878-886

SNPs and transcriptional activity of genes of innate and adaptive immunity at the maternal-fetal interface in woman with preterm labour, associated with preterm premature rupture of membranes.
Authors: Lyubomirskaya, Ekaterina S and Kamyshnyi, Alexandr M and Krut, Yuriy Ya and Smiianov, Vladyslav A and Fedoniuk, Larisa Ya and Romanyuk, Lidiya B and Kravets, Natalya Ya and Mochulska, Oksana M
Journal: Wiadomosci lekarskie (Warsaw, Poland : 1960) (2020): 25-30

Real-Time Reverse Transcription PCR as a Tool to Study Virulence Gene Regulation in Bacterial Pathogens.
Authors: Aviv, Gili and Gal-Mor, Ohad
Journal: Methods in molecular biology (Clifton, N.J.) (2018): 23-32

An improved RT-IPCR for detection of pyrene and related polycyclic aromatic hydrocarbons.
Authors: Meng, X Y and Li, Y S and Zhou, Y and Sun, Y and Qiao, B and Si, C C and Hu, P and Lu, S Y and Ren, H L and Liu, Z S and Qiu, H J and Liu, J Q
Journal: Biosensors & bioelectronics (2016): 194-199

Analysis of P. gingivalis, T. forsythia and S. aureus levels in edentulous mouths prior to and 6 months after placement of one-piece zirconia and titanium implants.
Authors: Siddiqi, Allauddin and Milne, Trudy and Cullinan, Mary P and Seymour, Gregory J
Journal: Clinical oral implants research (2016): 288-94

Real-time immuno-PCR for ultrasensitive detection of pyrene and other homologous PAHs.
Authors: Meng, X Y and Li, Y S and Zhou, Y and Zhang, Y Y and Qiao, B and Sun, Y and Yang, L and Hu, P and Lu, S Y and Ren, H L and Zhang, J H and Wang, X R and Liu, Z S
Journal: Biosensors & bioelectronics (2015): 42-7

Real-time polymerase chain reaction based on msa2c gene for detection of Babesia bovis.
Authors: Ramos, Carlos A N and Araújo, Flábio R and Souza, Ingrid I F and Bacanelli, G and Luiz, Hera L and Russi, Lívia S and Oliveira, Renato H M and Soares, Cleber O and Rosinha, Grácia M S and Alves, Leucio C
Journal: Veterinary parasitology (2011): 79-83

TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*

简要概述

产品基本信息

货号：17286

产品名称：TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*

规格：1ml

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

溶剂：水

产品介绍

用于 TaqMan 探针的 TAQuest qPCR Master Mix 是一种即用型 2X溶液，针对 qPCR 和与 TaqMan 基因表达分析兼容的两步法 RT-qPCR 进行了优化。预混液在优化的 PCR 缓冲液中提供了所有基本成分，包括我们专有的 TAQuest 热启动 Taq DNA 聚合酶和 dNTP，但模板、引物和探针除外。热启动 Taq DNA 聚合酶允许您在室温下设置 PCR 反应，从而最大限度地减少非特异性产物的形成。优化的成分可确保 PCR 对所有样本类型（如基因组、质粒、病毒和 cDNA 模板）的特异性和灵敏度。用于 TaqMan 探针的 TAQuest qPCR Master Mix 设计用于使用内部阳性对照的双链反应。该预混液含大量 ROX 参考染料。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*。 

适用仪器

样品实验方案
注意 在室温下解冻TAQuest qPCR Master Mix 用于TaqMan探针*高ROX*。 使用前彻底涡旋 qPCR Master Mix。
1. 制备表 1 所示的下列反应混合物之一。
2. 轻轻涡旋混合试剂，然后短暂离心。
3. 在 qPCR 仪器中设置板并按表 2 所示操作。

表 1. 各反应每孔试剂组成

表 2. 热循环参数

参考文献

Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation.
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