上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
胱天蛋白酶Caspase抑制剂Z-LETD-FMK
 |
货号 | 13307 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 1 mg | 价格 | 1200 | |
| Ex (nm) | Em (nm) | |||
| 分子量 | 654.69 | 溶剂 | DMSO | |
| 产品详细介绍 | ||||
简要概述
Z-LETD-FMK是一种细胞渗透性不可逆的caspase蛋白酶8抑制剂，与caspase-8的催化位点结合，在诱导凋亡中起重要作用。Z-LETD-FMK与caspase-8的结合抑制了该蛋白酶的活性，使其能够抑制与caspase-8激活相关的凋亡事件。为了抑制细胞凋亡，应在诱导细胞凋亡的同时添加Z-LETD-FMK。FMK衍生的肽可作为有效的不可逆抑制剂，而不会增加细胞毒性作用。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Z-LETD-FMK。
产品说明书
样品实验方案
以下是我们推荐的方案，仅提供指导。具体实验应根据您的特定需求进行修改。
溶液配制
储备溶液配制
操作步骤
- 根据需要处理样品以诱导凋亡。
- 添加Z-LETD-FMK。
注意：我们建议使用最终浓度为10-100 µM的Z-LETD-FMK抑制胱天蛋白酶，例如对caspase-8抑制50 µM。
注意：孵育的最佳时间和浓度需要通过实验确定。 - 根据需要对样品染色。
- 用适当的仪器检测荧光强度。
图示
 图1.用caspase 8抑制剂Z-LETD-FMK（Cat＃13307）抑制Jurkat细胞中caspase 8活性。当天，将Jurkat细胞以200,000个细胞/ 90 µL /孔的密度接种在Costar黑色96孔板中。用1 µM星形孢菌素处理细胞5小时。加入Z-LETD-FMK并与星形孢菌素共孵育。用Cell Meter Caspase 8活性细胞凋亡检测试剂盒（Cat#22816）检测caspase-8活性。用Flexstation 3荧光酶标仪（Molecular Devices）在Ex / Em ＝ 540 / 620nm（截止＝ 610nm）下测量荧光强度。 |
参考文献
Rapamycin, an mTOR inhibitor, induced apoptosis via independent mitochondrial and death receptor pathway in retinoblastoma Y79 cell.
Authors: Wang, Yan-Dong and Su, Yong-Jing and Li, Jian-Ying and Yao, Xiang-Chao and Liang, Guang-Jiang
Journal: International journal of clinical and experimental medicine (2015): 10723-30
A polysaccharide from Sanguisorbae radix induces caspase-dependent apoptosis in human leukemia HL-60 cells.
Authors: Wu, Zhigang and Sun, Honghui and Li, Jingzhong and Ma, Chijiao and Zhao, Siqiao and Guo, Zheng and Lin, Yao and Lin, Yaping and Liu, Li
Journal: International journal of biological macromolecules (2014): 615-20
Use of fluorochrome-labeled inhibitors of caspases to detect neuronal apoptosis in the whole-mounted lamprey brain after spinal cord injury.
Authors: Barreiro-Iglesias, Antón and Shifman, Michael I
Journal: Enzyme research (2012): 835731
Caspase-8 and p38MAPK in DATS-induced apoptosis of human CNE2 cells.
Authors: Ji, C and Ren, F and Xu, M
Journal: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas (2010): 821-7
[Synergistic effects and mechanisms of combined tumor necrosis factor-related apoptosis-inducing ligand and chemotherapeutic drugs or radiotherapy in killing laryngeal squamous carcinoma cells in vitro].
Authors: Zhang, Ming and Zhou, Liang
Journal: Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery (2009): 565-70
Emodin azide methyl anthraquinone derivative triggers mitochondrial-dependent cell apoptosis involving in caspase-8-mediated Bid cleavage.
Authors: Yan, Yanyan and Su, Xiaodong and Liang, Yongju and Zhang, Jianye and Shi, Chengjun and Lu, Yu and Gu, Lianquan and Fu, Liwu
Journal: Molecular cancer therapeutics (2008): 1688-97
Caspase-9 takes part in programmed cell death in developing mouse kidney.
Authors: Araki, Takashi and Hayashi, Matsuhiko and Nakanishi, Keiko and Morishima, Nobuhiro and Saruta, Takao
Journal: Nephron. Experimental nephrology (2003): e117-24
Induction of apoptosis in human leukemia cells by the CDK1 inhibitor CGP74514A.
Authors: Dai, Yun and Dent, Paul and Grant, Steven
Journal: Cell cycle (Georgetown, Tex.): 143-52
