核酸染料 LDS 651

	货号	17566	存储条件	在零下15度以下保存, 避免光照
	规格	5 mg	价格	1164
	Ex (nm)	557	Em (nm)	630
	分子量	473.40	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：17566

产品名称：核酸染料LDS 651

规格：5mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：473.40

外观：固体

溶剂：DMSO

产品介绍

LDS 651是LDS 751的类似物。LDS 651具有与LDS 751几乎相同的细胞内染色模式。LDS 651经过优化，可被紫色激光激发，而LDS 751被蓝激光在488 nm激发。 LDS 651和LDS 751都是可透过细胞的荧光染料。 尽管LDS 651和LDS 751均与DNA结合，但它们可能会从细胞核中流出，并与其他细胞结构（如线粒体的极化膜）结合。 当将LDS 651、LDS 751用于分析活细胞核状态时，应格外注意。 LDS 651和LDS 751可用于将红细胞和有核细胞分离。

参考文献

Quantifying permeabilization and activity recovery of Bacillus spores in adverse conditions for growth.
Authors: Trunet, C and Ngo, H and Coroller, L
Journal: Food microbiology (2019): 115-120

Calcium Ionophore, Calcimycin, Kills Leishmania Promastigotes by Activating Parasite Nitric Oxide Synthase.
Authors: Grekov, Igor and Pombinho, António R and Kobets, Tatyana and Bartůněk, Petr and Lipoldová, Marie
Journal: BioMed research international (2017): 1309485

Lipid bilayer properties control membrane partitioning, binding, and transport of p-glycoprotein substrates.
Authors: Clay, Adam T and Sharom, Frances J
Journal: Biochemistry (2013): 343-54

Interaction of LDS-751 with the drug-binding site of P-glycoprotein: a Trp fluorescence steady-state and lifetime study.
Authors: Lugo, Miguel R and Sharom, Frances J
Journal: Archives of biochemistry and biophysics (2009): 17-28

Flow cytometric assessment of homeostatic aging of reticulocytes in rats.
Authors: Wiczling, Paweł and Krzyzanski, Wojciech
Journal: Experimental hematology (2008): 119-27

A novel flow cytometric assay of human whole blood neutrophil and monocyte CD11b levels: upregulation by chemokines is related to receptor expression, comparison with neutrophil shape change, and effects of a chemokine receptor (CXCR2) antagonist.
Authors: Nicholson, Grant C and Tennant, Rachel C and Carpenter, Donald C and Sarau, Henry M and Kon, Onn Min and Barnes, Peter J and Salmon, Michael and Vessey, Rupert S and Tal-Singer, Ruth and Hansel, Trevor T
Journal: Pulmonary pharmacology & therapeutics (2007): 52-9

Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry.
Authors: Maes, Melissa L and Davidson, Lisa B and McDonagh, Paul F and Ritter, Leslie S
Journal: Journal of immunological methods (2007): 79-86

Determination of the formation of dark state via depleted spontaneous emission in a complex solvated molecule.
Authors: Guo, Xunmin and Wang, Sufan and Xia, Andong and Su, Hongmei
Journal: The journal of physical chemistry. A (2007): 5800-5

Cell cycle synchronization of Cupriavidus necator by continuous phasing measured via flow cytometry.
Authors: Fritsch, M and Starruss, J and Loesche, A and Mueller, S and Bley, Th
Journal: Biotechnology and bioengineering (2005): 635-42

Interaction of LDS-751 and rhodamine 123 with P-glycoprotein: evidence for simultaneous binding of both drugs.
Authors: Lugo, Miguel R and Sharom, Frances J
Journal: Biochemistry (2005): 14020-9

核酸染料 LDS 751 CAS 181885-68-7

	货号	17561	存储条件	在零下15度以下保存, 避免光照
	规格	25 mg	价格	4836
	Ex (nm)	561	Em (nm)	712
	分子量	471.98	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：17561

产品名称：核酸染料LDS 751

CAS：181885-68-7

规格：25mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：471.98

外观：深绿色粉末

溶剂：DMSO

激发波长(nm)：561

发射波长(nm)：712

产品介绍

LDS 751是一种渗透性细胞核酸染料，它在dsDNA上在〜543 nm处具有峰值激发，但可以用488 nm激光很好地激发。它是DRAQ 5 的替代产品，由于其最大的最大波长发射波长（〜712 nm），可用于多色分析。与LDS 751的dsDNA结合后，荧光增强程度约为20倍。LDS-751不进入细胞核，并与线粒体膜结合。因此，当使用LDS 751分析活细胞和使用LDS-751探针检测细胞时需要清楚，它不可用于细胞核检测。噻唑橙（RNA染料）和LDS-751（DNA染料）组合物已被用于将网织红细胞与其他细胞群分离。 LDS 751也已用于从有核细胞中分离红细胞。

点击查看光谱

参考文献

Exciton-controlled fluorescence: application to hybridization-sensitive fluorescent DNA probe
Authors: Okamoto A, Ikeda S, Kubota T, Yuki M, Yanagisawa H.
Journal: Nucleic Acids Symp Ser (Oxf) (2009): 49

Hybridization-sensitive fluorescent probe for long-term monitoring of intracellular RNA
Authors: Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A.
Journal: Bioconjug Chem (2009): 1256

Interaction of LDS-751 with the drug-binding site of P-glycoprotein: a Trp fluorescence steady-state and lifetime study
Authors: Lugo MR, Sharom FJ.
Journal: Arch Biochem Biophys (2009): 17

Flow cytometric assessment of homeostatic aging of reticulocytes in rats
Authors: Wiczling P, Krzyzanski W.
Journal: Exp Hematol (2008): 119

Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry
Authors: Maes ML, Davidson LB, McDonagh PF, Ritter LS.
Journal: J Immunol Methods (2007): 79

Determination of the formation of dark state via depleted spontaneous emission in a complex solvated molecule
Authors: Guo X, Wang S, Xia A, Su H.
Journal: J Phys Chem A (2007): 5800

Cell cycle synchronization of Cupriavidus necator by continuous phasing measured via flow cytometry
Authors: Fritsch M, Starruss J, Loesche A, Mueller S, Bley T.
Journal: Biotechnol Bioeng (2005): 635

Exciton mobility and trapping in a MALDI matrix
Authors: Setz PD, Knochenmuss R.
Journal: J Phys Chem A (2005): 4030

Interaction of LDS-751 and rhodamine 123 with P-glycoprotein: evidence for simultaneous binding of both drugs
Authors: Lugo MR, Sharom FJ.
Journal: Biochemistry (2005): 14020

Quantitative evaluation of isothiocyanates as substrates and inhibitors of P-glycoprotein
Authors: Barecki-Roach M, Wang EJ, Johnson WW.
Journal: J Pharm Pharmacol (2003): 1251

产品说明书

染色细胞分析方案

注意：以下协议适用于大多数细胞类型。 生长培养基，细胞密度，其他细胞类型和因子的存在可能影响染色。 玻璃器皿上的残留洗涤剂也可能影响许多生物的染色，并导致在有或没有细胞存在的溶液中出现明亮染色的物质。

1.制备5-10 mM DMSO储备溶液。

2.向细胞（悬浮细胞或贴壁细胞）中加入1至10μM，并将细胞染色15至60分钟。 在最初的实验中，最好尝试几种染料浓度来确定产生所需结果的最佳浓度。 高染料浓度倾向于引起其他细胞结构的非特异性染色。

3.用荧光显微镜，荧光酶标仪或流式细胞仪直接分析细胞染色。

参考文献

Exciton-controlled fluorescence: application to hybridization-sensitive fluorescent DNA probe
Authors: Okamoto A, Ikeda S, Kubota T, Yuki M, Yanagisawa H.
Journal: Nucleic Acids Symp Ser (Oxf) (2009): 49

Hybridization-sensitive fluorescent probe for long-term monitoring of intracellular RNA
Authors: Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A.
Journal: Bioconjug Chem (2009): 1256

Interaction of LDS-751 with the drug-binding site of P-glycoprotein: a Trp fluorescence steady-state and lifetime study
Authors: Lugo MR, Sharom FJ.
Journal: Arch Biochem Biophys (2009): 17

Flow cytometric assessment of homeostatic aging of reticulocytes in rats
Authors: Wiczling P, Krzyzanski W.
Journal: Exp Hematol (2008): 119

Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry
Authors: Maes ML, Davidson LB, McDonagh PF, Ritter LS.
Journal: J Immunol Methods (2007): 79

Determination of the formation of dark state via depleted spontaneous emission in a complex solvated molecule
Authors: Guo X, Wang S, Xia A, Su H.
Journal: J Phys Chem A (2007): 5800

Cell cycle synchronization of Cupriavidus necator by continuous phasing measured via flow cytometry
Authors: Fritsch M, Starruss J, Loesche A, Mueller S, Bley T.
Journal: Biotechnol Bioeng (2005): 635

Exciton mobility and trapping in a MALDI matrix
Authors: Setz PD, Knochenmuss R.
Journal: J Phys Chem A (2005): 4030

Interaction of LDS-751 and rhodamine 123 with P-glycoprotein: evidence for simultaneous binding of both drugs
Authors: Lugo MR, Sharom FJ.
Journal: Biochemistry (2005): 14020

Quantitative evaluation of isothiocyanates as substrates and inhibitors of P-glycoprotein
Authors: Barecki-Roach M, Wang EJ, Johnson WW.
Journal: J Pharm Pharmacol (2003): 1251

核酸染料 LDS 751 CAS 181885-68-7

	货号	17567	存储条件	在零下15度以下保存, 避免光照
	规格	5 mg	价格	1164
	Ex (nm)	561	Em (nm)	712
	分子量	471.98	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：17567

产品名称：核酸染料LDS 751

CAS：181885-68-7

规格：5mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：471.98

外观：深绿色粉末

溶剂：水或DMSO

激发波长(nm)：543

发射波长(nm)：712

产品介绍

LDS 751是一种渗透性细胞核酸染料，它在dsDNA上在〜543 nm处具有峰值激发，但可以用488 nm激光很好地激发。它是DRAQ 5 的替代产品，由于其最大的最大波长发射波长（〜712 nm），可用于多色分析。与LDS 751的dsDNA结合后，荧光增强程度约为20倍。LDS-751不进入细胞核，并与线粒体膜结合。因此，当使用LDS 751分析活细胞和使用LDS-751探针检测细胞时需要清楚，它不可用于细胞核检测。噻唑橙（RNA染料）和LDS-751（DNA染料）组合物已被用于将网织红细胞与其他细胞群分离。 LDS 751也已用于从有核细胞中分离红细胞。

点击查看光谱

参考文献

Exciton-controlled fluorescence: application to hybridization-sensitive fluorescent DNA probe
Authors: Okamoto A, Ikeda S, Kubota T, Yuki M, Yanagisawa H.
Journal: Nucleic Acids Symp Ser (Oxf) (2009): 49

Hybridization-sensitive fluorescent probe for long-term monitoring of intracellular RNA
Authors: Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A.
Journal: Bioconjug Chem (2009): 1256

Interaction of LDS-751 with the drug-binding site of P-glycoprotein: a Trp fluorescence steady-state and lifetime study
Authors: Lugo MR, Sharom FJ.
Journal: Arch Biochem Biophys (2009): 17

Flow cytometric assessment of homeostatic aging of reticulocytes in rats
Authors: Wiczling P, Krzyzanski W.
Journal: Exp Hematol (2008): 119

Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry
Authors: Maes ML, Davidson LB, McDonagh PF, Ritter LS.
Journal: J Immunol Methods (2007): 79

Determination of the formation of dark state via depleted spontaneous emission in a complex solvated molecule
Authors: Guo X, Wang S, Xia A, Su H.
Journal: J Phys Chem A (2007): 5800

Cell cycle synchronization of Cupriavidus necator by continuous phasing measured via flow cytometry
Authors: Fritsch M, Starruss J, Loesche A, Mueller S, Bley T.
Journal: Biotechnol Bioeng (2005): 635

Exciton mobility and trapping in a MALDI matrix
Authors: Setz PD, Knochenmuss R.
Journal: J Phys Chem A (2005): 4030

Interaction of LDS-751 and rhodamine 123 with P-glycoprotein: evidence for simultaneous binding of both drugs
Authors: Lugo MR, Sharom FJ.
Journal: Biochemistry (2005): 14020

Quantitative evaluation of isothiocyanates as substrates and inhibitors of P-glycoprotein
Authors: Barecki-Roach M, Wang EJ, Johnson WW.
Journal: J Pharm Pharmacol (2003): 1251

核酸染料 LDS 651

	货号	17566	存储条件	在零下15度以下保存, 避免光照
	规格	5 mg	价格	1164
	Ex (nm)	557	Em (nm)	630
	分子量	473.40	溶剂	DMSO
	产品详细介绍

简要概述

产品基本信息

货号：17566

产品名称：核酸染料LDS 651

规格：5mg

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

分子量：473.40

外观：固体

溶剂：DMSO

产品介绍

LDS 651是LDS 751的类似物。LDS 651具有与LDS 751几乎相同的细胞内染色模式。LDS 651经过优化，可被紫色激光激发，而LDS 751被蓝激光在488 nm激发。 LDS 651和LDS 751都是可透过细胞的荧光染料。 尽管LDS 651和LDS 751均与DNA结合，但它们可能会从细胞核中流出，并与其他细胞结构（如线粒体的极化膜）结合。 当将LDS 651、LDS 751用于分析活细胞核状态时，应格外注意。 LDS 651和LDS 751可用于将红细胞和有核细胞分离。

参考文献

Quantifying permeabilization and activity recovery of Bacillus spores in adverse conditions for growth.
Authors: Trunet, C and Ngo, H and Coroller, L
Journal: Food microbiology (2019): 115-120

Calcium Ionophore, Calcimycin, Kills Leishmania Promastigotes by Activating Parasite Nitric Oxide Synthase.
Authors: Grekov, Igor and Pombinho, António R and Kobets, Tatyana and Bartůněk, Petr and Lipoldová, Marie
Journal: BioMed research international (2017): 1309485

Lipid bilayer properties control membrane partitioning, binding, and transport of p-glycoprotein substrates.
Authors: Clay, Adam T and Sharom, Frances J
Journal: Biochemistry (2013): 343-54

Interaction of LDS-751 with the drug-binding site of P-glycoprotein: a Trp fluorescence steady-state and lifetime study.
Authors: Lugo, Miguel R and Sharom, Frances J
Journal: Archives of biochemistry and biophysics (2009): 17-28

Flow cytometric assessment of homeostatic aging of reticulocytes in rats.
Authors: Wiczling, Paweł and Krzyzanski, Wojciech
Journal: Experimental hematology (2008): 119-27

A novel flow cytometric assay of human whole blood neutrophil and monocyte CD11b levels: upregulation by chemokines is related to receptor expression, comparison with neutrophil shape change, and effects of a chemokine receptor (CXCR2) antagonist.
Authors: Nicholson, Grant C and Tennant, Rachel C and Carpenter, Donald C and Sarau, Henry M and Kon, Onn Min and Barnes, Peter J and Salmon, Michael and Vessey, Rupert S and Tal-Singer, Ruth and Hansel, Trevor T
Journal: Pulmonary pharmacology & therapeutics (2007): 52-9

Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry.
Authors: Maes, Melissa L and Davidson, Lisa B and McDonagh, Paul F and Ritter, Leslie S
Journal: Journal of immunological methods (2007): 79-86

Determination of the formation of dark state via depleted spontaneous emission in a complex solvated molecule.
Authors: Guo, Xunmin and Wang, Sufan and Xia, Andong and Su, Hongmei
Journal: The journal of physical chemistry. A (2007): 5800-5

Cell cycle synchronization of Cupriavidus necator by continuous phasing measured via flow cytometry.
Authors: Fritsch, M and Starruss, J and Loesche, A and Mueller, S and Bley, Th
Journal: Biotechnology and bioengineering (2005): 635-42

Interaction of LDS-751 and rhodamine 123 with P-glycoprotein: evidence for simultaneous binding of both drugs.
Authors: Lugo, Miguel R and Sharom, Frances J
Journal: Biochemistry (2005): 14020-9