上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
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货号 | 50567 | 存储条件 | |
| 规格 | 1 mg | 价格 | 5940 | |
| Ex (nm) | Em (nm) | |||
| 分子量 | N/A | 溶剂 | ||
| 产品详细介绍 | ||||
简要概述
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
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货号 | 50573 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 5 mg | 价格 | 11628 | |
| Ex (nm) | Em (nm) | |||
| 分子量 | 溶剂 | Water | ||
| 产品详细介绍 | ||||
简要概述
产品基本信息
货号：50573
产品名称：盐霉素-KLH缀合物
规格：5mg
储存条件：-15℃避光
保质期：12个月
产品介绍
盐霉素是一种由白色链霉菌产生的聚醚单羧酸。 它可用于预防因猪瘟，中肠埃里氏菌，大头孢菌，布鲁内特氏菌，大肠埃希菌和大肠埃希菌引起的家禽球虫病。 沙利霉素对某些革兰氏阳性细菌具有活性，而肠杆菌科则具有抗性。 盐霉素对产气荚膜梭菌也有抑制作用，因此使用盐霉素可降低肉鸡坏死性肠炎的发生率。盐霉素被认为是标记残留物，皮肤/脂肪被认为是靶组织。与其他聚醚离子载体相比，盐霉素在鸡蛋中产生的残留水平相对较低。盐霉素-KLH缀合物是通过ELISA定量盐霉素的基本试剂。
参考文献
Molecular characterization and analysis of the ATPase ASNA1 homolog gene of Eimeria tenella in a drug sensitive strain and drug resistant strains.
Authors: Yu, Yu and Dong, Hui and Zhao, Qiping and Zhu, Shunhai and Liang, Shanshan and Wang, Qingjie and Wang, Haixia and Yu, Shuilan and Huang, Bing and Han, Hongyu
Journal: International journal for parasitology. Drugs and drug resistance (2021): 115-125
Molecular characterization and functional analysis of Eimeria tenella citrate synthase.
Authors: Wang, Haixia and Zhao, Qiping and Zhu, Shunhai and Dong, Hui and Yu, Shuilan and Wang, Qingjie and Yu, Yu and Liang, Shanshan and Zhao, Huanzhi and Huang, Bing and Han, Hongyu
Journal: Parasitology research (2021): 1025-1035
Targeted Delivery of Combination Therapeutics Using Monoclonal Antibody 2C5-Modified Immunoliposomes for Cancer Therapy.
Authors: Narayanaswamy, Radhika and Torchilin, Vladimir P
Journal: Pharmaceutical research (2021): 429-450
Enhanced targeting of prostate cancer-initiating cells by salinomycin-encapsulated lipid-PLGA nanoparticles linked with CD44 antibodies.
Authors: Wei, Jun and Sun, Jin and Liu, Yu
Journal: Oncology letters (2019): 4024-4033
Vasculogenic mimicry is associated with trastuzumab resistance of HER2-positive breast cancer.
Authors: Hori, Ami and Shimoda, Masafumi and Naoi, Yasuto and Kagara, Naofumi and Tanei, Tomonori and Miyake, Tomohiro and Shimazu, Kenzo and Kim, Seung Jin and Noguchi, Shinzaburo
Journal: Breast cancer research : BCR (2019): 88
Promoted delivery of salinomycin sodium to lung cancer cells by dual targeting PLGA hybrid nanoparticles.
Authors: Zhou, Jie and Sun, Jin and Chen, Huaiwen and Peng, Qing
Journal: International journal of oncology (2018): 1289-1300
The enhanced delivery of salinomycin to CD133+ ovarian cancer stem cells through CD133 antibody conjugation with poly(lactic-co-glycolic acid)-poly(ethylene glycol) nanoparticles.
Authors: Mi, Yi and Huang, Yuqin and Deng, Jie
Journal: Oncology letters (2018): 6611-6621
CD44 targeted chemotherapy for co-eradication of breast cancer stem cells and cancer cells using polymeric nanoparticles of salinomycin and paclitaxel.
Authors: Muntimadugu, Eameema and Kumar, Rajendra and Saladi, Shantikumar and Rafeeqi, Towseef Amin and Khan, Wahid
Journal: Colloids and surfaces. B, Biointerfaces (2016): 532-546
Combination of drug-conjugated SWCNT nanocarriers for efficient therapy of cancer stem cells in a breast cancer animal model.
Authors: Al Faraj, Achraf and Shaik, Asma Sultana and Ratemi, Elaref and Halwani, Rabih
Journal: Journal of controlled release : official journal of the Controlled Release Society (2016): 240-51
Laser-induced fluorescence reader with a turbidimetric system for sandwich-type immunoassay using nanoparticles.
Authors: Kim, Y H and Lim, H B
Journal: Analytica chimica acta (2015): 32-6
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
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货号 | 50005 | 存储条件 | 在零下15度以下保存 |
| 规格 | 1 mg | 价格 | 6564 | |
| Ex (nm) | Em (nm) | |||
| 分子量 | 溶剂 | |||
| 产品详细介绍 | ||||
简要概述
产品基本信息
货号：50005
产品名称：3-氨基-2-恶唑烷（AOZ）-KLH缀合物
规格：1mg
储存条件：-15℃避光防潮
保质期：12个月
产品介绍
3-Amino-2-oxazolidine（AOZ）-KLH缀合物用于开发抗3-Amino-2-oxazolidine（AOZ）抗体，通常在ELISA分析中使用。 3-Amino-2-oxazolidine（AOZ）-BSA共轭物在每个KLH上具有多个3-Amino-2-oxazolidine（AOZ）分子，以最大化其免疫原性。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的3-氨基-2-恶唑烷（AOZ）-KLH缀合物。
参考文献
A high-throughput, precipitating colorimetric sandwich ELISA microarray for shiga toxins
Authors: Gehring A, He X, Fratamico P, Lee J, Bagi L, Brewster J, Paoli G, He Y, Xie Y, Skinner C, Barnett C, Harris D.
Journal: Toxins (Basel) (2014): 1855
Development of antigen capture ELISA for the quantification of EIAV p26 protein
Authors: Hu Z, Chang H, Ge M, Lin Y, Wang X, Guo W.
Journal: Appl Microbiol Biotechnol (2014): 9073
Development of atom transfer radical polymer-modified gold nanoparticle-based enzyme-linked immunosorbent assay (ELISA)
Authors: Chen F, Hou S, Li Q, Fan H, Fan R, Xu Z, Zhala G, Mai X, Chen X, Liu Y.
Journal: Anal Chem (2014): 10021
Development of monoclonal antibody-based sandwich ELISA for detection of dextran
Authors: Wang SY, Li Z, Wang XJ, Lv S, Yang Y, Zeng LQ, Luo FH, Yan JH, Liang DF.
Journal: Monoclon Antib Immunodiagn Immunother (2014): 334
Effect of different combinations of antibodies and enzyme labels on ELISA of progesterone
Authors: Kumari GL, P and ey PK, Nathsharma SS, Sharma SK, Kochhar G.
Journal: J Immunoassay Immunochem (2014): 157
Long-term dry storage of an enzyme-based reagent system for ELISA in point-of-care devices
Authors: Ramach and ran S, Fu E, Lutz B, Yager P.
Journal: Analyst (2014): 1456
Ultrasensitive ELISA using enzyme-loaded nanospherical brushes as labels
Authors: Qu Z, Xu H, Xu P, Chen K, Mu R, Fu J, Gu H.
Journal: Anal Chem (2014): 9367
3-(10′-Phenothiazinyl)propionic acid is a potent primary enhancer of peroxidase-induced chemiluminescence and its application in sensitive ELISA of methylglyoxal-modified low density lipoprotein
Authors: Sakharov IY, Demiyanova AS, Gribas AV, Uskova NA, Efremov EE, Vdovenko MM.
Journal: Talanta (2013): 414
Development of an ELISA for the quantification of the C-terminal decapeptide prothymosin alpha(100-109) in sera of mice infected with bacteria
Authors: Samara P, Kalbacher H, Ioannou K, Radu DL, Livaniou E, Promponas VJ, Voelter W, Tsitsilonis O.
Journal: J Immunol Methods (2013): 54
ELISA for determination of total coagulation factor XII concentration in human plasma
Authors: Madsen DE, Sidelmann JJ, Overgaard K, Koch C, Gram JB.
Journal: J Immunol Methods (2013): 32
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
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货号 | 50002 | 存储条件 | 在零下15度以下保存 |
| 规格 | 1 mg | 价格 | 6564 | |
| Ex (nm) | Em (nm) | |||
| 分子量 | 溶剂 | |||
| 产品详细介绍 | ||||
简要概述
产品基本信息
货号：50002
产品名称：氯霉素-KLH缀合物
规格：1mg
储存条件：-15℃避光防潮
保质期：12个月
产品介绍
氯霉素-KLH缀合物用于开发通常在ELISA分析中使用的抗氯霉素抗体。 氯霉素-KLH共轭物在每个KLH上具有多个氯霉素分子，以最大化其免疫原性。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的氯霉素-KLH缀合物。
参考文献
A high-throughput, precipitating colorimetric sandwich ELISA microarray for shiga toxins
Authors: Gehring A, He X, Fratamico P, Lee J, Bagi L, Brewster J, Paoli G, He Y, Xie Y, Skinner C, Barnett C, Harris D.
Journal: Toxins (Basel) (2014): 1855
Development of antigen capture ELISA for the quantification of EIAV p26 protein
Authors: Hu Z, Chang H, Ge M, Lin Y, Wang X, Guo W.
Journal: Appl Microbiol Biotechnol (2014): 9073
Development of atom transfer radical polymer-modified gold nanoparticle-based enzyme-linked immunosorbent assay (ELISA)
Authors: Chen F, Hou S, Li Q, Fan H, Fan R, Xu Z, Zhala G, Mai X, Chen X, Liu Y.
Journal: Anal Chem (2014): 10021
Development of monoclonal antibody-based sandwich ELISA for detection of dextran
Authors: Wang SY, Li Z, Wang XJ, Lv S, Yang Y, Zeng LQ, Luo FH, Yan JH, Liang DF.
Journal: Monoclon Antib Immunodiagn Immunother (2014): 334
Effect of different combinations of antibodies and enzyme labels on ELISA of progesterone
Authors: Kumari GL, P and ey PK, Nathsharma SS, Sharma SK, Kochhar G.
Journal: J Immunoassay Immunochem (2014): 157
Long-term dry storage of an enzyme-based reagent system for ELISA in point-of-care devices
Authors: Ramach and ran S, Fu E, Lutz B, Yager P.
Journal: Analyst (2014): 1456
Ultrasensitive ELISA using enzyme-loaded nanospherical brushes as labels
Authors: Qu Z, Xu H, Xu P, Chen K, Mu R, Fu J, Gu H.
Journal: Anal Chem (2014): 9367
3-(10′-Phenothiazinyl)propionic acid is a potent primary enhancer of peroxidase-induced chemiluminescence and its application in sensitive ELISA of methylglyoxal-modified low density lipoprotein
Authors: Sakharov IY, Demiyanova AS, Gribas AV, Uskova NA, Efremov EE, Vdovenko MM.
Journal: Talanta (2013): 414
Development of an ELISA for the quantification of the C-terminal decapeptide prothymosin alpha(100-109) in sera of mice infected with bacteria
Authors: Samara P, Kalbacher H, Ioannou K, Radu DL, Livaniou E, Promponas VJ, Voelter W, Tsitsilonis O.
Journal: J Immunol Methods (2013): 54
ELISA for determination of total coagulation factor XII concentration in human plasma
Authors: Madsen DE, Sidelmann JJ, Overgaard K, Koch C, Gram JB.
Journal: J Immunol Methods (2013): 32
上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
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货号 | 5502 | 存储条件 | Multiple |
| 规格 | 2 Reactions | 价格 | 2604 | |
| Ex (nm) | Em (nm) | |||
| 分子量 | 溶剂 | |||
| 产品详细介绍 | ||||
简要概述
ReadiLink KLH偶联试剂盒 是美国AAT Bioquest生产的KLH偶联试剂盒，钉形贝血蓝蛋白 (KLH)是一种最常用的结合多肽的抗体产品载体。海洋生物养殖性钉形贝血蓝蛋白 (mcKLH) 是从海洋生贝壳类软体动物提取的血蓝蛋白与KLH免疫原性属性类似但作为载体蛋白生产的半抗原抗体和多肽更稳定和高效。它包含许多单元，使用活性胺基或活性羧基交联反应，有效结合每个分子肽和其他抗原。因此mcKLH是一个受欢迎的载体蛋白，mcKLH接合半抗原和其他不稳定的抗原生产更多免疫原性的抗体产品 。这一 ReadiLink™ KLH交联试剂盒首要是优化简单的半抗原载体轭合物免疫原性的抗体产品的制备。 ReadiLink™ KLH 交联试剂盒是一步法结合的半抗原载体蛋白，是羧基活性碳化二亚胺的交联剂。这一共轭形式被用于诱发免疫反应和半抗原的抗体生产。羧基活性碳化二亚胺与暴露在稳定的多肽和蛋白质表面的羧基和氨基基团反应。这一试剂盒包含 mcKLH和与之相配的缓冲溶液及兼容羧基活性碳化二亚胺反应和淡化旋转列,能恢复通过离心步骤的蛋白质源的形态。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的ReadiLink KLH偶联试剂盒。
产品说明书
样品实验方案
简要概述
1.准备蛋白质溶液
2.准备半抗原解决方案
3.将蛋白质与半抗原混合到EDC中
4.在室温下孵育反应2小时
5.通过脱盐来纯化缀合物
工作溶液配制
1. mcKLH溶液（10 mg / mL）：
将200μLddH2 O加入到mcKLH（组分A）的小瓶中以制备10mg / mL溶液。 注意：mcKLH溶液通常呈半透明至白蓝色。 不要涡旋或加热溶液，否则会使载体沉淀。
2. Hapten工作溶液：
在450μL缀合缓冲液（组分B）中溶解最多2mg半抗原。 注意：一些半抗原可能具有有限的溶解度，使用DMSO（在最终的缀合溶液中≤30％）首先溶解它。 较高浓度的DMSO可能使载体蛋白不可逆地变性。
3. KLH-Hapten工作溶液：
将450μL半抗原溶液加入200μLmcKLH溶液中，得到KLH-Hapten工作溶液。
操作步骤
1.KLH-Hapten结合
1.1将一小瓶EDC（组分C，10mg）溶解在1mL ddH 2 O中，立即将50μL该溶液加入KLH-Hapten工作溶液中，轻轻混合。在室温下孵育2小时。通过脱盐纯化缀合物以除去未反应的交联剂和蛋白质防腐剂（例如叠氮化钠）。
1.2拧下脱盐柱（组件E）的底部盖子，然后松开盖子。将色谱柱放入收集管中。
1.3将柱以1,000g离心2分钟以除去储存溶液。
1.4取下盖子，慢慢地向柱子中加入1 mL纯化缓冲液。以1,000g离心2分钟，取出缓冲液。再重复此步骤3次，从收集管中丢弃缓冲液。
1.5将色谱柱放入新的收集管中，轻轻地将样品放入紧凑树脂床的中心。
1.6将柱以1,000g离心2分钟以收集样品。
1.7KLH-Hapten缀合物现在可用于免疫。如果KLH-Hapten缀合物要储存超过几天，则无菌过滤缀合物，并储存在4°C或-20°C。注意：如果在一周内使用缀合物，可以使用PBS进行纯化。如果缀合物将被冷冻，则使用纯化缓冲盐（组分D）进行纯化。如果在缀合中使用DMSO，则制备具有相同百分比的用于缀合的DMSO的纯化缓冲盐。这将使脱盐期间塔中的沉淀最小化。如果在缀合过程中形成沉淀，则将沉淀的材料离心，收集上清液并保存沉淀物。净化上清液。将沉淀物与纯化的缀合物合并。
数据分析
| 图1.EDC与载体蛋白BSA或KLH（由红球代表）的羧基反应，形成胺反应性O-酰基异脲中间体（中心分子）。 O-酰基异脲中间体与由较小的蓝色球代表的抗原分子上的胺基反应，产生通过稳定的酰胺键连接的两个分子的缀合物[请注意O-酰基异脲中间体也易于水解]。 |
参考文献
Optimization of the preparation process of vinblastine sulfate (VBLS)-loaded folate-conjugated bovine serum albumin (BSA) nanoparticles for tumor-targeted drug delivery using response surface methodology (RSM)
Authors: Zu Y, Zhang Y, Zhao X, Zhang Q, Liu Y, Jiang R.
Journal: Int J Nanomedicine (2009): 321
Characterization of bovine serum albumin glycated with glucose, galactose and lactose
Authors: Ledesma-Osuna AI, Ramos-Clamont G, Vazquez-Moreno L.
Journal: Acta Biochim Pol (2008): 491
Improvement of functional properties of bovine serum albumin through phosphorylation by dry-heating in the presence of pyrophosphate
Authors: Enomoto H, Li CP, Morizane K, Ibrahim HR, Sugimoto Y, Ohki S, Ohtomo H, Aoki T.
Journal: J Food Sci (2008): C84
Polyethylene glycol improves conjugation of bovine hemoglobin and human serum albumin in a controlled ratio
Authors: Zheng C, Bi J, Ma G, Su Z.
Journal: Artif Cells Blood Substit Immobil Biotechnol (2007): 568
Purification of denatured bovine serum albumin coated CdTe quantum dots for sensitive detection of silver(I) ions
Authors: Wang JH, Wang HQ, Zhang HL, Li XQ, Hua XF, Cao YC, Huang ZL, Zhao YD.
Journal: Anal Bioanal Chem (2007): 969
Effect of lyophilization on the structure and phase changes of PEGylated-bovine serum albumin
Authors: Tattini V, Jr., Parra DF, Polakiewicz B, Pitombo RN.
Journal: Int J Pharm (2005): 124
[Preparation of bovine serum albumin nanoparticles surface-modified with glycyrrhizin]
Authors: Mao SJ, Hou SX, Zhang LK, Jin H, Bi YQ, Jiang B.
Journal: Yao Xue Xue Bao (2003): 787
[Products of spontaneous conjugation of aflatoxins with bovine serum albumin: immunochemical properties]
Authors: Burkin AA, Kononenko GP, Soboleva NA.
Journal: Prikl Biokhim Mikrobiol (2003): 228
Generation of polyclonal antibody against mu-conotoxin GIIIA using an immunogen of [Cys(5)]mu-conotoxin GIIIA site-specifically conjugated with bovine serum albumin
Authors: Nakamura M, Oba Y, Mori T, Sato K, Ishida Y, Matsuda T, Nakamura H.
Journal: Biochem Biophys Res Commun (2002): 1037
Mice vaccinated with the O-antigen of Francisella tularensis LVS lipopolysaccharide conjugated to bovine serum albumin develop varying degrees of protective immunity against systemic or aerosol challenge with virulent type A and type B strains of the pathogen
Authors: Conlan JW, Shen H, Webb A, Perry MB.
Journal: Vaccine (2002): 3465
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