Gelite 红色核酸凝胶染色剂 10,000X DMSO溶液 (停产) 货号17600-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Gelite 红色核酸凝胶染色剂 10,000X DMSO溶液 (停产)

Gelite 红色核酸凝胶染色剂 10,000X DMSO溶液 (停产)

Gelite 红色核酸凝胶染色剂 10,000X DMSO溶液 (停产)    货号17600 货号 17600 存储条件 在零下15度以下保存, 避免光照
规格 1 mL 价格 0
Ex (nm) 538 Em (nm) 609
分子量 溶剂
产品详细介绍

简要概述

产品基本信息

货号:17600

产品名称:Gelite 红色核酸凝胶染色剂 10,000X DMSO溶液

规格:1ml

储存条件:保存在冰箱-15℃避光干燥

保质期:12个月

 

产品物理化学光谱特性

外观:液体

激发波长(nm):538

发射波长(nm):609

 

产品介绍

        Gelite 红色核酸凝胶染色剂 10,000X DMSO溶液 是美国AAT Bioquest生产的核酸染料,Gelite Red是我们Gelite 核酸凝胶染色家族的最新成员。现在,我们拥有一整套多色凝胶染色剂,用于检测凝胶中的核酸。Gelite Red是一种极其敏感的核酸凝胶染色剂,用于使用标准的300 nm UV透射仪和Polaroid 667黑白打印胶片检测凝胶中的DNA。在相同条件下,它比流行的SYBR Gold凝胶染色剂更敏感。这种显着的敏感性可归因于Gelite Red的独特染料特性的组合。因为核酸结合的Gelite Red染料显示出接近488nm和~300nm的激发最大值(发射最大值为~610nm),与各种仪器兼容,包括紫外线外延和透射仪和蓝光透射仪,以及基于汞弧灯和氩离子激光的凝胶扫描仪。我们的Gelite Red是SYBR Gold,SYBR Safe和Ethidium Bromide的优质替代品。它为染色凝胶中的核酸样品提供了方便的解决方案。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Gelite 红色核酸凝胶染色剂。 

 

图示

Gelite 红色核酸凝胶染色剂 10,000X DMSO溶液 (停产)    货号17600

凝胶染色后Gelite Red和SYBR Gold的比较。 将1kb Plus DNA Ladder(每泳道200ng和100ng)在1×TAE缓冲液中的0.9%琼脂糖中进行电泳。 随后将凝胶在1x TAE中用1x Gelite Red或SYBR TM Gold染色30分钟,并使用具有EtBr滤光剂的ChemiDoc MP Imager拍照。

 

相似产品(点击查看)

 

参考文献

Ethidium DNA agarose gel electrophoresis: how it started
Authors: P. Borst
Journal: IUBMB Life (2005): 745-7

Comparison of ethidium bromide-stained agarose gel electrophoresis and automated fragment analysis for evaluation of IgH gene products
Authors: B. Gleissner
Journal: Leuk Res (2001): 769-74

An enzymatic procedure for the purification of DNA restriction fragments without gel electrophoresis and ethidium bromide staining
Authors: D. Ratel
Journal: C R Acad Sci III (2000): 753-6

Comparison of SYBR Green I nucleic acid gel stain mutagenicity and ethidium bromide mutagenicity in the Salmonella/mammalian microsome reverse mutation assay (Ames test)
Authors: V. L. Singer
Journal: Mutat Res (1999): 37-47

Effects of ethidium bromide on DNA loop organisation in human lymphocytes measured by anomalous viscosity time dependence and single cell gel electrophoresis
Authors: I. Y. Belyaev
Journal: Biochim Biophys Acta (1999): 348-56

Genotyping microsatellite polymorphisms by agarose gel electrophoresis with ethidium bromide staining: application to quantitative trait loci analysis of seizure susceptibility in mice
Authors: T. N. Ferraro
Journal: Psychiatr Genet (1998): 227-33

The effect of ethidium bromide on mobility of DNA fragments in agarose gel electrophoresis
Authors: J. Sigmon
Journal: Electrophoresis (1996): 1524-7

Fluorescent labeling of DNA with ethidium homodimer without measurable decrease in DNA mobility: application to automated gel electrophoresis apparatus
Authors: S. F. Zakharov
Journal: Anal Biochem (1995): 195-8

Ethidium bromide staining during denaturation with glyoxal for sensitive detection of RNA in agarose gel electrophoresis
Authors: D. Grundemann
Journal: Anal Biochem (1994): 459-61

High-sensitivity two-color detection of double-stranded DNA with a confocal fluorescence gel scanner using ethidium homodimer and thiazole orange
Authors: H. S. Rye
Journal: Nucleic Acids Res (1991): 327-33

说明书
Gelite 红色核酸凝胶染色剂 10,000X DMSO溶液 (停产).pdf

Gelite Safe核酸凝胶染料* 10,000X DMSO溶液* 货号17705-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Gelite Safe核酸凝胶染料* 10,000X DMSO溶液*

Gelite Safe核酸凝胶染料* 10,000X DMSO溶液*

Gelite Safe核酸凝胶染料* 10,000X DMSO溶液*    货号17705 货号 17705 存储条件 室温储存不要冷冻, 避免光照
规格 500 ul 价格 1164
Ex (nm) 513 Em (nm) 552
分子量 N/A 溶剂 Water
产品详细介绍

简要概述

Gelite Safe 核酸凝胶染料是一种非常敏感且稳定的荧光染料,可用于电泳凝胶中的DNA检测。 与高毒性的溴化乙锭(EtBr)不同,Gelite Safe经过专门设计,具有较低的危害性,无细胞毒性和诱变性,与EtBr和SYBR®Safe相比,具有更高的灵敏度和更低的背景荧光。 Gelite Safe的独特光谱特性扩展了其仪器兼容性,包括紫外和蓝光透射仪,凝胶记录系统和激光扫描仪。无需繁杂的操作,可在在绿色或红色通道中进行检测。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Gelite Safe核酸凝胶染料* 10,000X DMSO溶液*。 

点击查看光谱

 

适用仪器


凝胶成像仪  
激发(nm): 紫外透射仪/蓝色激发光
发射(nm): SYBR® 滤波片, GelStar® 滤波片, GelGreen® 滤波片, or GelRed® 滤波片

产品说明书

样品实验方案

工作溶液配制

Gelite Safe 工作溶液

在7.5-8.5的pH范围内使用(例如,TAE、TBE或TE pH 8.2)缓冲液稀释10,000X储备试剂来制备1X Gelite Safe工作溶液。
注意:在水中配制的染色溶液比在缓冲液中配制的溶液不稳定,必须在24小时内使用。

 

后染色方案
1.根据您的标准方案运行凝胶。
2.将凝胶放在合适的聚丙烯容器中。 轻轻加入足量的1X染色溶液以浸没凝胶。
注意:请勿使用玻璃容器,因为它会吸收染色溶液中的许多染料。
3.在室温下避光轻轻搅拌凝胶约30至60分钟。
4.使用300 nm / 254 nm紫外透射仪或使用长距离绿色滤光片(例如SYBR®滤光片,GelStar®滤光片,GelGreen®滤光片或GelRed®滤光片)的凝胶扫描仪对凝胶成像。

 

预染色方案
1.使用标准方案准备琼脂糖凝胶溶液。
2.在倒入凝胶之前,将10,000X Gelite Safe储备试剂以1:10,000的比例稀释到凝胶溶液中并彻底混合。
3.根据您的标准方案运行凝胶。
4.使用300 nm / 254 nm紫外透射仪或使用长距离绿色滤光片(例如SYBR®滤光片,GelStar®滤光片,GelGreen®滤光片或GelRed®滤光片)的凝胶扫描仪对凝胶成像。

 

图示

Gelite Safe核酸凝胶染料* 10,000X DMSO溶液*    货号17705

图1.使用Gelite Safe,EtBr和SYBR®Safe在TBE缓冲液中的1%琼脂糖凝胶中检测DNA的比较。 从左到右分别以100 ng,50 ng和25 ng的量加载1 kb DNA的两倍连续稀释液。 根据制造商推荐的浓度,将凝胶用Gelite Safe,EtBr和SYBR®Safe染色60分钟,并使用ChemiDoc 成像系统(Bio-Rad®)进行成像。 使用装有GelGreen和GelRed滤光片的300 nm荧光透射仪对凝胶进行照明。

 

参考文献

Limited Proteolysis and Gel Electrophoresis in the Presence of Metal Cations: Au(III)-binding Luminescent Domain in Serum Albumins.
Authors: Dixon, Jacob M and Egusa, Shunji
Journal: Journal of visualized experiments : JoVE (2021)

High affinity of AS1411 toward copper; its application in a sensitive aptasensor for copper detection.
Authors: Bahreyni, Amirhossein and Ramezani, Mohammad and Alibolandi, Mona and Hassanzadeh, Pirooz and Abnous, Khalil and Taghdisi, Seyed Mohammad
Journal: Analytical biochemistry (2019): 1-9

The efficacy and tolerability of 5-aminolevulinic acid 5% thermosetting gel photodynamic therapy (PDT) in the treatment of mild-to-moderate acne vulgaris. A two-center, prospective assessor-blinded, proof-of-concept study.
Authors: Serini, Stefano Maria and Cannizzaro, Maria Vittoria and Dattola, Annunziata and Garofalo, Virginia and Del Duca, Esther and Ventura, Alessandra and Milani, Massimo and Campione, Elena and Bianchi, Luca
Journal: Journal of cosmetic dermatology (2019): 156-162

Transfection Studies with Colloidal Systems Containing Highly Purified Bipolar Tetraether Lipids from Sulfolobus acidocaldarius.
Authors: Engelhardt, Konrad H and Pinnapireddy, Shashank Reddy and Baghdan, Elias and Jedelská, Jarmila and Bakowsky, Udo
Journal: Archaea (Vancouver, B.C.) (2017): 8047149

Metallo-supramolecular gels based on a multitopic cyclam bis-terpyridine platform.
Authors: Gasnier, Aurélien and Royal, Guy and Terech, Pierre
Journal: Langmuir : the ACS journal of surfaces and colloids (2009): 8751-62

Characterization of hepatic L-threonine dehydrogenase of chicken.
Authors: Yuan, J H and Austic, R E
Journal: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology (2001): 65-73

[Cloning of group A streptococcal pyrogenic exotoxin-B gene and its recombinant protein expression in culture supernatant].
Authors: Watanabe, Y
Journal: Journal of Nippon Medical School = Nippon Ika Daigaku zasshi (2001): 222-32

Purification and characterization of chondroitin 4-sulfotransferase from the culture medium of a rat chondrosarcoma cell line.
Authors: Yamauchi, S and Hirahara, Y and Usui, H and Takeda, Y and Hoshino, M and Fukuta, M and Kimura, J H and Habuchi, O
Journal: The Journal of biological chemistry (1999): 2456-63

Comparison of tube and gel red blood cell agglutination techniques in detecting chimeras after major ABO-mismatched allogeneic hematopoietic stem cell transplantation.
Authors: Kupferman, M J and Cipolone, K M and Procter, J L and Stroncek, D F
Journal: Immunohematology (1998): 63-7

Expression of pig heart mitochondrial NADP-dependent isocitrate dehydrogenase in Escherichia coli.
Authors: Soundar, S and Jennings, G T and McAlister-Henn, L and Colman, R F
Journal: Protein expression and purification (1996): 305-12

说明书
Gelite Safe核酸凝胶染料* 10,000X DMSO溶液*.pdf

Gelite™ Safe DNA Gel Stain *GelRed Replacement, 10,000X in DMSO* 货号17709-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Gelite™ Safe DNA Gel Stain *GelRed Replacement, 10,000X in DMSO*

Gelite™ Safe DNA Gel Stain *GelRed Replacement, 10,000X in DMSO*

货号 17709 存储条件 在零下15度以下保存, 避免光照
规格 0.5 mL 价格 1164
Ex (nm) 513 Em (nm) 552
分子量 N/A 溶剂 Water
产品详细介绍

简要概述

Gelite 橙色核酸凝胶染色试剂盒 货号17594-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Gelite 橙色核酸凝胶染色试剂盒

Gelite 橙色核酸凝胶染色试剂盒

Gelite 橙色核酸凝胶染色试剂盒     货号17594 货号 17594 存储条件 室温储存不要冷冻, 避免光照
规格 1 Kit 价格 2604
Ex (nm) 495 Em (nm) 540
分子量 溶剂
产品详细介绍

简要概述

产品基本信息

货号:17594

产品名称:Gelite 橙色核酸凝胶染色试剂盒

规格:1kit

储存条件:保存在冰箱-15℃避光干燥

保质期:12个月

 

产品物理化学光谱特性

外观:液体

激发波长(nm):495

发射波长(nm):540

 

适用仪器


透射仪  
激发: 254nm or 300nm
发射: long Path 绿色滤波片(如:SYBR或GelStar)

 

产品介绍

Gelite 橙色核酸凝胶染色试剂盒 是美国AAT Bioquest生产的核酸染料,Cyber Orange 是一种极其敏感的核酸凝胶染料,可使用标准的300 nm UV透照仪和Polaroid 667黑白印刷胶片检测凝胶中的DNA或RNA。 与Cyber Green 染色剂一样,这种出色的敏感性可以归因于独特的染料特性的结合。 由于与核酸结合的Cyber Orange 染料在〜495 nm和〜300 nm处均显示出最大激发光(最大发射量为〜537 nm),因此它可与多种仪器兼容,包括紫外线落射照明器和透射照明器以及 蓝光透射灯,以及基于汞弧灯和氩离子激光的凝胶扫描仪。我们的Gelite 橙色核酸凝胶染色凝胶试剂盒包括我们的Cyber Orange 核酸染色剂,具有优化且稳定的操作流程。 它为凝胶中的核酸样品染色提供了方便的操作方案。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Gelite 橙色核酸凝胶染色试剂盒 。 

产品说明书

样品实验方案

溶液配制

工作溶液

将1μLGelite 橙色(组分A)添加到200μL5X凝胶上样缓冲液(组分B)中。 通过用箔纸覆盖或将其置于黑暗中来保护工作溶液免受光照。

 

操作步骤

1.根据需要准备DNA样品。

2.将4 µL Gelite Orange工作溶液加入16 µL DNA样品中,并充分混合。 电泳前,在室温下孵育5-15分钟。

3.根据您的标准方案运行凝胶。

4.使用300 nm紫外线或254 nm透照器或使用长路径绿色滤光片(例如SYBR®滤光片或GelStar®滤光片)的基于激光的凝胶扫描仪对凝胶成像。

 

参考文献

A Broadly Reactive One-Step SYBR Green I Real-Time RT-PCR Assay for Rapid Detection of Murine Norovirus
Authors: Hanaki K, Ike F, Kajita A, Yasuno W, Yanagiba M, Goto M, Sakai K, Ami Y, Kyuwa S.
Journal: PLoS One (2014): e98108

A SYBR Green I based real time RT-PCR assay for specific detection and quantitation of Peste des petits ruminants virus
Authors: Abera T, Thangavelu A, Joy Chandran ND, Raja A.
Journal: BMC Vet Res (2014): 22

A SYBR-green I quantitative real-time reverse transcription-PCR assay for rabies viruses with different virulence
Authors: Wang L, Liu Y, Zhang S, Wang Y, Zhao J, Miao F, Hu R.
Journal: Virol Sin (2014): 131

Comparison of SYBR Green and TaqMan methods in quantitative real-time polymerase chain reaction analysis of four adenosine receptor subtypes
Authors: Tajadini M, Panjehpour M, Javanmard SH.
Journal: Adv Biomed Res (2014): 85

Detection and characterization of Leishmania (Leishmania) and Leishmania (Viannia) by SYBR green-based real-time PCR and high resolution melt analysis targeting kinetoplast minicircle DNA
Authors: Ceccarelli M, Galluzzi L, Migliazzo A, Magnani M.
Journal: PLoS One (2014): e88845

Detection of Cardamom mosaic virus and Banana bract mosaic virus in cardamom using SYBR Green based reverse transcription-quantitative PCR
Authors: Siljo A, Bhat AI, Biju CN.
Journal: Virusdisease (2014): 137

Development and comparative evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of West Nile virus in human patients
Authors: Kumar JS, Saxena D, Parida M.
Journal: Mol Cell Probes. (2014)

Development and evaluation of SYBR Green-I based quantitative PCR assays for herpes simplex virus type 1 whole transcriptome analysis
Authors: Garvey CE, McGowin CL, Foster TP.
Journal: J Virol Methods (2014): 101

Development of a High-resolution Melting Analysis Method Based on SYBR Green-I for rs7216389 Locus Genotyping in Asthmatic Child Patients
Authors: Vali Z, Raz A, Bokharaei H, Nabavi M, Bemanian MH, Yazdi MS, Djadid ND.
Journal: Avicenna J Med Biotechnol (2014): 72

Development of a SYBR Green I based one-step real-time PCR assay for the detection of Hantaan virus
Authors: Jiang W, Wang PZ, Yu HT, Zhang Y, Zhao K, Du H, Bai XF.
Journal: J Virol Methods (2014): 145

 

相关产品

产品名称 货号
Gelite 绿色核酸凝胶染色试剂盒 Cat#17589

说明书
Gelite 橙色核酸凝胶染色试剂盒 .pdf

Gelite Safe核酸凝胶染料* 10,000X水溶液* 货号17700-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Gelite Safe核酸凝胶染料* 10,000X水溶液*

Gelite Safe核酸凝胶染料* 10,000X水溶液*

Gelite Safe核酸凝胶染料* 10,000X水溶液*    货号17700 货号 17700 存储条件 室温储存不要冷冻, 避免光照
规格 100 ul 价格 924
Ex (nm) 513 Em (nm) 552
分子量 N/A 溶剂 Water
产品详细介绍

简要概述

Gelite Safe 核酸凝胶染料是一种非常敏感且稳定的荧光染料,可用于电泳凝胶中的DNA检测。 与高毒性的溴化乙锭(EtBr)不同,Gelite Safe经过专门设计,具有较低的危害性,无细胞毒性和诱变性,与EtBr和SYBR®Safe相比,具有更高的灵敏度和更低的背景荧光。 Gelite Safe的独特光谱特性扩展了其仪器兼容性,包括紫外和蓝光透射仪,凝胶记录系统和激光扫描仪。无需繁杂的操作,可在在绿色或红色通道中进行检测。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Gelite Safe核酸凝胶染料* 10,000X水溶液*。 

点击查看光谱

 

适用仪器


凝胶成像仪  
激发(nm): 紫外透射仪/蓝色激发光
发射(nm): SYBR® 滤波片, GelStar® 滤波片, GelGreen® 滤波片, or GelRed® 滤波片

产品说明书

样品实验方案

工作溶液配制

Gelite Safe 工作溶液

在7.5-8.5的pH范围内使用(例如,TAE、TBE或TE pH 8.2)缓冲液稀释10,000X储备试剂来制备1X Gelite Safe工作溶液。
注意:在水中配制的染色溶液比在缓冲液中配制的溶液不稳定,必须在24小时内使用。

 

后染色方案
1.根据您的标准方案运行凝胶。
2.将凝胶放在合适的聚丙烯容器中。 轻轻加入足量的1X染色溶液以浸没凝胶。
注意:请勿使用玻璃容器,因为它会吸收染色溶液中的许多染料。
3.在室温下避光轻轻搅拌凝胶约30至60分钟。
4.使用300 nm / 254 nm紫外透射仪或使用长距离绿色滤光片(例如SYBR®滤光片,GelStar®滤光片,GelGreen®滤光片或GelRed®滤光片)的凝胶扫描仪对凝胶成像。

 

预染色方案
1.使用标准方案准备琼脂糖凝胶溶液。
2.在倒入凝胶之前,将10,000X Gelite Safe储备试剂以1:10,000的比例稀释到凝胶溶液中并彻底混合。
3.根据您的标准方案运行凝胶。
4.使用300 nm / 254 nm紫外透射仪或使用长距离绿色滤光片(例如SYBR®滤光片,GelStar®滤光片,GelGreen®滤光片或GelRed®滤光片)的凝胶扫描仪对凝胶成像。

 

图示

Gelite Safe核酸凝胶染料* 10,000X水溶液*    货号17700

图1.使用Gelite Safe,EtBr和SYBR®Safe在TBE缓冲液中的1%琼脂糖凝胶中检测DNA的比较。 从左到右分别以100 ng,50 ng和25 ng的量加载1 kb DNA的两倍连续稀释液。 根据制造商推荐的浓度,将凝胶用Gelite Safe,EtBr和SYBR®Safe染色60分钟,并使用ChemiDoc 成像系统(Bio-Rad®)进行成像。 使用装有GelGreen和GelRed滤光片的300 nm荧光透射仪对凝胶进行照明。

 

参考文献

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说明书
Gelite Safe核酸凝胶染料* 10,000X水溶液*.pdf