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FIASH二乙酸酯
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货号 | 22334 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 100 Tests | 价格 | 4836 | |
| Ex (nm) | 498 | Em (nm) | 517 | |
| 分子量 | 748.56 | 溶剂 | DMSO | |
| 产品详细介绍 | ||||
简要概述
产品基本信息
货号：22334
产品名称：FIASH二乙酸酯
规格：100 Tests
储存条件：-20℃干燥
保质期：12个月
产品物理化学光谱特性
外观：固体
分子量：784.56
溶剂：DMSO
Ex(nm):498
Em(nm):517
适用仪器
| 荧光显微镜 | |
| 激发： | FITC滤波片组 |
| 发射： | FITC滤波片组 |
| 推荐孔板： | 黑色透明 |
产品介绍
FlASH 二乙酸酯是FlASH的可渗透细胞的形式，它是一种荧光素衍生物，经修饰可包含两个彼此相距一定距离的砷原子。它由Roger Tsien及其同事在1998年开发。双砷标记技术通过砷与硫醇的高亲和力相互作用起作用。当FlASH结合四半胱氨酸（TC）序列时，其双砷基与Cys-Cys部分快速反应，并且标签变为绿色的高度荧光。双砷标记试剂FIASH是用于标记包含具有Cys-Cys-X1-X2-Cys-Cys氨基酸序列的六氨基酸基序的蛋白质的最小表达标签。最常用的四半胱氨酸是六个氨基酸的Cys-Cys-Pro-Gly-Cys-Cys序列。由于此序列很少出现在内源蛋白中，将序列整合到靶蛋白中会产生一个小的但高度特异性的蛋白标记靶。当与包含四半胱氨酸基序Cys-Cys-Pro-Gly-Cys-Cys的重组蛋白结合时，FIASH会产生强烈的绿色荧光信号。它可以用于检测蛋白质的定位，周转和运输，受体信号传导和内在化。
参考文献
Fluorescent labelling of membrane fatty acid transporter CD36 (SR-B2) in the extracellular loop.
Authors: Liu, Yilin and Rodriguez-Calvo, Ricardo and Wang, Shujin and Zhu, Xiaoqing and Broers, Jos L V and Glatz, Jan F C and Luiken, Joost J F P and Neumann, Dietbert
Journal: PloS one (2019): e0210704
Probing Arrestin Function Using Intramolecular FlAsH-BRET Biosensors.
Authors: Strungs, Erik G and Luttrell, Louis M and Lee, Mi-Hye
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 309-322
Probing the Effect of Sildenafil on Progesterone and Testosterone Production by an Intracellular FRET/BRET Combined Approach.
Authors: Casarini, Livio and Riccetti, Laura and Limoncella, Silvia and Lazzaretti, Clara and Barbagallo, Federica and Pacifico, Salvatore and Guerrini, Remo and Tagliavini, Simonetta and Trenti, Tommaso and Simoni, Manuela and Sola, Marco and Di Rocco, Giulia
Journal: Biochemistry (2019): 799-808
Using Tetracysteine-Tagged TDP-43 with a Biarsenical Dye To Monitor Real-Time Trafficking in a Cell Model of Amyotrophic Lateral Sclerosis.
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Journal: Biochemistry (2019): 4086-4095
The novel mitochondria localization of influenza A virus NS1 visualized by FlAsH labeling.
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Journal: FEBS open bio (2017): 1960-1971
Universal genetic assay for engineering extracellular protein expression.
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Acute inactivation of PSD-95 destabilizes AMPA receptors at hippocampal synapses.
Authors: Yudowski, Guillermo A and Olsen, Olav and Adesnik, Hillel and Marek, Kurt W and Bredt, David S
Journal: PloS one (2013): e53965
Site-specific labeling of the type 1 ryanodine receptor using biarsenical fluorophores targeted to engineered tetracysteine motifs.
Authors: Fessenden, James D and Mahalingam, Mohana
Journal: PloS one (2013): e64686
Localization of Aggregatibacter actinomycetemcomitans cytolethal distending toxin subunits during intoxication of live cells.
Authors: Damek-Poprawa, Monika and Jang, Jae Yeon and Volgina, Alla and Korostoff, Jonathan and DiRienzo, Joseph M
Journal: Infection and immunity (2012): 2761-70
Superresolution imaging of HIV in infected cells with FlAsH-PALM.
Authors: Lelek, Mickaël and Di Nunzio, Francesca and Henriques, Ricardo and Charneau, Pierre and Arhel, Nathalie and Zimmer, Christophe
Journal: Proceedings of the National Academy of Sciences of the United States of America (2012): 8564-9
