5-FAM 5-羧基荧光素炔烃 货号951-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

5-FAM 5-羧基荧光素炔烃

5-FAM 5-羧基荧光素炔烃

5-FAM 5-羧基荧光素炔烃    货号951 货号 951 存储条件
规格 100 mg 价格 9948
Ex (nm) 493 Em (nm) 517
分子量 413.38 溶剂
产品详细介绍

简要概述

产品基本信息

货号:951

产品名称:5-FAM 5-羧基荧光素炔烃

规格:100mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:413.38

溶剂:DMSO

激发波长(nm):492

发射波长(nm):516

 

产品介绍

5-FAM 5-羧基荧光素炔烃是美国AAT Bioquest生产的荧光染料。5-FAM炔烃是通过与叠氮基反应开发荧光生物共轭物的优异构件。 5-FAM是羧基荧光素的纯化单一异构体。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的5-FAM 5-羧基荧光素炔烃。 

点击查看光谱

 

参考文献

Multicellular Vascularized Engineered Tissues through User-Programmable Biomaterial Photodegradation
Authors: Christopher K Arakawa, Barry A Badeau, Ying Zheng, Cole A DeForest
Journal: Advanced Materials (2017)

Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure
Authors: Yu-Hsuan Peng, Douglas H Sweet, Shiuan-Pey Lin, Chung-Ping Yu, Pei-Dawn Lee Chao, Yu-Chi Hou
Journal: Scientific reports (2015)

Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Søren Preus, L Marcus Wilhelmsson
Journal: ChemBioChem (2012): 1990–2001

 

相关产品

产品名称 货号
5-FAM 5-羧基荧光素尸胺 Cat#128
5-FAM 5-羧基荧光素叠氮化物 Cat#131
5-FAM 5-羧基荧光素 Cat#103

说明书
5-FAM 5-羧基荧光素炔烃.pdf

荧光素醛[5-FAM醛] 货号9001-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

荧光素醛[5-FAM醛]

荧光素醛[5-FAM醛]

荧光素醛[5-FAM醛]    货号9001 货号 9001 存储条件
规格 1 mg 价格 2388
Ex (nm) 493 Em (nm) 517
分子量 550.52 溶剂
产品详细介绍

简要概述

产品基本信息

货号:9001

产品名称:荧光素醛[5-FAM醛]

规格:1mg

储存条件:保存在冰箱-15℃干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:550.52

外观:固体

溶剂:DMSO

激发波长(nm):493

发射波长(nm):517

 

产品介绍

荧光素醛是一种活性荧光染料,可与胺、肼或羟胺反应。 荧光素是应用最广泛的绿色荧光染料。 醛基对 pH 5-9 的胺、酰肼或羟胺基具有反应性。 与胺反应性琥珀酰亚胺酯基团 (NHS) 不同,醛可以在酸性 pH 下与 N 端胺基反应,某些生物偶联反应有时需要这种条件。 醛与胺基反应形成中间席夫键。 用氢化物进一步还原将形成稳定的 C-N 键。 醛和其他基团之间的反应允许荧光素染料在目标分子上的所需位置进行位点特异性结合和标记。 该染料可以通过 FITC 通道下的普通荧光仪器轻松检测。

点击查看光谱

 

参考文献

2-Formyl-dATP as Substrate for Polymerase Synthesis of Reactive DNA Bearing an Aldehyde Group in the Minor Groove.
Authors: Krömer, Matouš and Brunderová, Mária and Ivancová, Ivana and Poštová Slavětínská, Lenka and Hocek, Michal
Journal: ChemPlusChem (2020): 1164-1170

Aldehyde dehydrogenase-2 activation decreases acetaminophen hepatotoxicity by prevention of mitochondrial depolarization.
Authors: Wimborne, Hereward J and Hu, Jiangting and Takemoto, Kenji and Nguyen, Nga T and Jaeschke, Hartmut and Lemasters, John J and Zhong, Zhi
Journal: Toxicology and applied pharmacology (2020): 114982

Bisretinoid phospholipid and vitamin A aldehyde: Shining a light.
Authors: Kim, Hye Jin and Sparrow, Janet R
Journal: Journal of lipid research (2020)

Characterization of ascites-derived aldehyde dehydrogenase-positive ovarian cancer stem cells isolated from Leghorn chickens.
Authors: Tiwari, Anupama and Hadley, Jill A and Ramachandran, Ramesh
Journal: Poultry science (2020): 2203-2214

Citral Inhibition of Human Salivary Aldehyde Dehydrogenase.
Authors: Laskar, Amaj Ahmed and Khan, Masood Alam and Ahmad, Sumbul and Hashmi, Amiruddin and Younus, Hina
Journal: Cell biochemistry and biophysics (2020): 31-42

Didactic approach recounting advances and limitations in novel glutathione and cysteine detection (reduced GSH probe) with mixed coumarin, aldehyde, and phenyl-selenium chemistry.
Authors: Lee, Woohyun and Mulay, Sandip V and Shimodaira, Shingo and Abdillah, Ariq and Palma, Jaymee and Kim, Youngsam and Yudhistira, Tesla and Churchill, David G
Journal: Methods in enzymology (2020): 267-289

Establishing sample-preparation protocols for X-ray phase-contrast CT of rodent spinal cords: Aldehyde fixations and osmium impregnation.
Authors: Barbone, Giacomo E and Bravin, Alberto and Mittone, Alberto and Kraiger, Markus J and Hrabě de Angelis, Martin and Bossi, Mario and Ballarini, Elisa and Rodriguez-Menendez, Virginia and Ceresa, Cecilia and Cavaletti, Guido and Coan, Paola
Journal: Journal of neuroscience methods (2020): 108744

Introduction of an Aldehyde Handle on Nanobodies by Affinity-Guided Labeling.
Authors: Mortensen, Michael R and Skovsgaard, Mikkel B and Märcher, Anders and Andersen, Veronica L and Palmfeldt, Johan and Nielsen, Thorbjørn B and Tørring, Thomas and Laursen, Nick S and Andersen, Kasper R and Kjems, Jørgen and Gothelf, Kurt V
Journal: Bioconjugate chemistry (2020): 1295-1300

Isocyanoacetate-Aldehyde Polymerization: A Facile Tool toward Functional Oxazoline-Containing Polymers.
Authors: Cheng, Tianyu and Chen, Yizhao and Ding, Jie and Qin, Anjun and Tang, Ben Zhong
Journal: Macromolecular rapid communications (2020): e2000179

Preparation and optical parameter characterization of two aldehyde derivative thin films for photonic applications by drop casting method.
Authors: Melavanki, Raveendra and Vaijayanthimala, S and Yallur, Basappa Chanabasappa and Shelar, Vikas M and Singh, Diksha and Sadasivuni, Kishor Kumar and Patil, N R
Journal: Luminescence : the journal of biological and chemical luminescence (2020): 903-912

说明书
荧光素醛[5-FAM醛].pdf

胱天蛋白酶Caspase抑制剂 FAM-VAD-FMK 货号13484-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

胱天蛋白酶Caspase抑制剂 FAM-VAD-FMK

胱天蛋白酶Caspase抑制剂 FAM-VAD-FMK

胱天蛋白酶Caspase抑制剂 FAM-VAD-FMK    货号13484 货号 13484 存储条件 在零下15度以下保存, 避免光照
规格 100 Tests 价格 2472
Ex (nm) 493 Em (nm) 517
分子量 691.66 溶剂 DMSO
产品详细介绍

简要概述

胱天蛋白酶Caspase抑制剂 FAM-VAD-FMK是美国AAT Bioquest生产的用于Caspase的试剂,FAM-VAD是一种绿色荧光细胞渗透性polycaspase抑制剂靶向胱天蛋白酶1,2,3,6,8,9或10。一旦进入细胞,所述抑制剂共价结合于胱天蛋白酶活性,其产生绿色荧光。当加入到细胞群体中,FAM-VAD-FMK探针进入每个细胞和共价结合到驻留在胱天蛋白酶活性的异二聚体的大亚基,从而抑制进一步的酶活性的反应性半胱氨酸残基。结合的标记试剂被保留在细胞内,而任何未结合的试剂将扩散出细胞,并被冲走。绿色荧光信号的直接测量活性胱天蛋白酶的存在于细胞中的试剂加入的时间量。含有结合的标记试剂细胞可以通过96 – 孔板的基于荧光,荧光显微镜或流式细胞仪进行分析。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的胱天蛋白酶Caspase抑制剂 FAM-VAD-FMK。 

点击查看光谱

产品说明书

样品实验方案 

溶液制备 

1.储备溶液

所有未使用的储备溶液应分为一次性使用的等分试样,并在制备后储存在-20°C下。 避免重复冻融循环。
1.1 FAM-VAD-FMK储备溶液:
在DMSO中准备2-5 mM的储备溶液。

 

样品操作及分析

1.根据需要处理细胞。

2.通过用20 mM Hepes缓冲液(HHBS)在Hanks中稀释DMSO储备溶液,制备2X渗透性半胱天冬酶底物(20 µM)分析溶液。

3.将等体积的处理过的细胞与2X半胱天冬酶底物测定溶液混合,并在37°C,5%CO2的培养箱中孵育细胞至少1小时。

4.用HHBS清洗细胞至少一次。

5.通过流式细胞仪,荧光显微镜或荧光酶标仪监测荧光强度。

 

参考文献

Suppression of human T cell proliferation by the caspase inhibitors, z-VAD-FMK and z-IETD-FMK is independent of their caspase inhibition properties
Authors: Lawrence CP, Chow SC.
Journal: Toxicol Appl Pharmacol. (2012)

[Inhibition of elicitation of allergic contact dermatitis by topical use of Z-VAD-FMK, a broad caspase inhibitor: experiment in mice]
Authors: Li YY, Yan CL.
Journal: Zhonghua Yi Xue Za Zhi (2012): 1992

Intracochlear perfusion of leupeptin and z-VAD-FMK: influence of antiapoptotic agents on gunshot-induced hearing loss
Authors: Abaamrane L, Raffin F, Schmerber S, Sendowski I.
Journal: Eur Arch Otorhinolaryngol (2011): 987

Plasmodium falciparum metacaspase PfMCA-1 triggers a z-VAD-fmk inhibitable protease to promote cell death
Authors: Meslin B, Beavogui AH, Fasel N, Picot S.
Journal: PLoS One (2011): e23867

Structure of human caspase-6 in complex with Z-VAD-FMK: New peptide binding mode observed for the non-canonical caspase conformation
Authors: Muller I, Lamers MB, Ritchie AJ, Dominguez C, Munoz-Sanjuan I, Kiselyov A.
Journal: Bioorg Med Chem Lett (2011): 5244

Pretreatment with pancaspase inhibitor (Z-VAD-FMK) delays but does not prevent intraperitoneal heat-killed group B Streptococcus-induced preterm delivery in a pregnant mouse model
Authors: Equils O, Moffatt-Blue C, Ishikawa TO, Simmons CF, Ilievski V, Hirsch E.
Journal: Infect Dis Obstet Gynecol (2009): 749432

[Attenuation of allergic contact dermatitis by Z-VAD-FMK, a broad caspase inhibitor: experiment with mice]
Authors: Li YY, Yan CL, Xu W.
Journal: Zhonghua Yi Xue Za Zhi (2008): 3153

Bodipy-VAD-Fmk, a useful tool to study yeast peptide N-glycanase activity
Authors: Witte MD, Descals CV, de Lavoir SV, Florea BI, van der Marel GA, Overkleeft HS.
Journal: Org Biomol Chem (2007): 3690

Caspase inhibitor Z-VAD-FMK enhances the freeze-thaw survival rate of human embryonic stem cells
Authors: Heng BC, Clement MV, Cao T.
Journal: Biosci Rep (2007): 257

Effects of caspase inhibitors (z-VAD-fmk, z-VDVAD-fmk) on Nile Red fluorescence pattern in 7-ketocholesterol-treated cells: investigation by flow cytometry and spectral imaging microscopy
Authors: Vejux A, Lizard G, Tourneur Y, Riedinger JM, Frouin F, Kahn E.
Journal: Cytometry A (2007): 550

 

相关产品

产品名称 货号
胱天蛋白酶Caspase 3/7荧光底物 FITC-C6-DEVD-FMK 绿 Cat#13406
胱天蛋白酶Caspase 3/7荧光底物(Z-DEVD)2-R110 绿 Cat#13430
胱天蛋白酶Caspase 3/7荧光底物Ac-DEVD-AMC 蓝 Cat#13402

说明书
胱天蛋白酶Caspase抑制剂 FAM-VAD-FMK.pdf

5-FAM 5-羧基荧光素 CAS 76823-03-5 货号103-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

5-FAM 5-羧基荧光素 CAS 76823-03-5

5-FAM 5-羧基荧光素 CAS 76823-03-5

5-FAM 5-羧基荧光素 CAS 76823-03-5    货号103 货号 103 存储条件 在零下15度以下保存, 避免光照
规格 100 mg 价格 1008
Ex (nm) 493 Em (nm) 517
分子量 376.32 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:103

产品名称:5-FAM [5-羧基荧光素] 

CAS:76823-03-5

规格:100mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:376.32

外观:橙色固体     

溶剂:DMSO

激发波长(nm):492

发射波长(nm):518

 

产品介绍

5-FAM 5-羧基荧光素是美国AAT Bioquest生产的荧光素,5-FAM是羧基荧光素的纯化单一异构体。 它是用于标记肽,蛋白质和核苷酸的最流行的绿色荧光试剂之一。 它主要用于开发各种绿色荧光肽,可以用488nm的Ar激发光线激发,它还被用于制备各种小荧光分子。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的5-FAM 5-羧基荧光素。 

点击查看光谱

 

参考文献

Multicellular Vascularized Engineered Tissues through User-Programmable Biomaterial Photodegradation
Authors: Christopher K Arakawa, Barry A Badeau, Ying Zheng, Cole A DeForest
Journal: Advanced Materials (2017)

Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure
Authors: Yu-Hsuan Peng, Douglas H Sweet, Shiuan-Pey Lin, Chung-Ping Yu, Pei-Dawn Lee Chao, Yu-Chi Hou
Journal: Scientific reports (2015)

Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Søren Preus, L Marcus Wilhelmsson
Journal: ChemBioChem (2012): 1990–2001

 

相关产品

产品名称 货号
6-FAM亚磷酰胺单体 5’-荧光素氨基磷酸酯 Cat#6016
6-FAM 6-羧基荧光素 Cat#106
5-FAM,SE 5-羧基荧光素琥珀酰亚胺酯 Cat#113

说明书
5-FAM 5-羧基荧光素 CAS 76823-03-5.pdf

6-FAM 6-羧基荧光素叠氮化物 货号955-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

6-FAM 6-羧基荧光素叠氮化物

6-FAM 6-羧基荧光素叠氮化物

6-FAM 6-羧基荧光素叠氮化物    货号955 货号 955 存储条件
规格 100 mg 价格 9948
Ex (nm) 493 Em (nm) 517
分子量 444.40 溶剂
产品详细介绍

简要概述

产品基本信息

货号:955

产品名称:6-FAM 6-羧基荧光素叠氮化物

规格:100mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:444.40

溶剂:DMSO

激发波长(nm):492

发射波长(nm):516

 

产品介绍

6-FAM 6-羧基荧光素叠氮化物是美国AAT Bioquest生产的荧光染料。6-FAM叠氮化物是通过与炔基反应研发荧光生物共轭物的极好构件。它广泛用于标记寡核苷酸。 6-FAM是羧基荧光素的纯化单一异构体。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的6-FAM 6-羧基荧光素叠氮化物。 

点击查看光谱

 

参考文献

Multicellular Vascularized Engineered Tissues through User-Programmable Biomaterial Photodegradation
Authors: Christopher K Arakawa, Barry A Badeau, Ying Zheng, Cole A DeForest
Journal: Advanced Materials (2017)

Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure
Authors: Yu-Hsuan Peng, Douglas H Sweet, Shiuan-Pey Lin, Chung-Ping Yu, Pei-Dawn Lee Chao, Yu-Chi Hou
Journal: Scientific reports (2015)

Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Søren Preus, L Marcus Wilhelmsson
Journal: ChemBioChem (2012): 1990–2001

 

相关产品

产品名称 货号
6-FAM 6-羧基荧光素炔烃 Cat#134
5-FAM 5-羧基荧光素 Cat#103
6-FAM亚磷酰胺单体 5’-荧光素氨基磷酸酯 Cat#6016

说明书
6-FAM 6-羧基荧光素叠氮化物.pdf