标签归档：dctp

dCTP *100 mM PCR Grade* CAS 2056-98-6 货号17252-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

dCTP *100 mM PCR Grade* CAS 2056-98-6

dCTP *100 mM PCR Grade* CAS 2056-98-6

dCTP *100 mM PCR Grade* CAS 2056-98-6 货号17252 货号 17252 存储条件 在零下15度以下保存, 避免光照
规格 500 uL 价格 1164
Ex (nm) Em (nm)
分子量 467.16 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17252

产品名称：dCTP *100 mM PCR Grade*

CAS：2056-98-6

规格：500ul

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：467.16

外观：液体

溶剂：水

 

产品介绍

2′-脱氧胞苷-5′-三磷酸 (dCTP) 溶液用于 DNA 合成反应，如 PCR、DNA 测序和分子克隆技术。 这种 dCTP溶液适用于需要高质量试剂的许多应用。 此类程序包括逆转录 (RT)、聚合酶链反应 (PCR)、RT-PCR、DNA 标记反应和测序/循环测序分析。

 

参考文献

Enzymatic Synthesis of Chimeric DNA Oligonucleotides by in Vitro Transcription with dTTP, dCTP, dATP, and 2′-Fluoro Modified dGTP.
Authors: Kapustina, Žana and Jasponė, Aistė and Dubovskaja, Varvara and Mackevičius, Gytis and Lubys, Arvydas
Journal: ACS synthetic biology (2021): 1625-1632

Polymerase synthesis of four-base DNA from two stable dimeric nucleotides.
Authors: Mohsen, Michael G and Ji, Debin and Kool, Eric T
Journal: Nucleic acids research (2019): 9495-9501

Self-Priming Enzymatic Fabrication of Multiply Modified DNA.
Authors: Whitfield, Colette J and Little, Rachel C and Khan, Kasid and Ijiro, Kuniharu and Connolly, Bernard A and Tuite, Eimer M and Pike, Andrew R
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2018): 15267-15274

Flexible double-headed cytosine-linked 2′-deoxycytidine nucleotides. Synthesis, polymerase incorporation to DNA and interaction with DNA methyltransferases.
Authors: Kielkowski, Pavel and Cahová, Hana and Pohl, Radek and Hocek, Michal
Journal: Bioorganic & medicinal chemistry (2016): 1268-76

PCR amplification of GC-rich DNA regions using the nucleotide analog N4-methyl-2′-deoxycytidine 5′-triphosphate.
Authors: Flores-Juárez, Cyntia R and González-Jasso, Eva and Antaramian, Anaid and Pless, Reynaldo C
Journal: BioTechniques (2016): 175-182

Fluorescence polarization-based method with bisulfite conversion-specific one-label extension for quantification of single CpG dinucleotide methylation.
Authors: Li, Shufen and Wang, Zhongju and Zhou, Lin and Luo, Fu and Zhao, Cunyou
Journal: Genome (2015): 357-63

Hi-C in Budding Yeast.
Authors: Belton, Jon-Matthew and Dekker, Job
Journal: Cold Spring Harbor protocols (2015): 649-61

Molecular identification of Anisakis and Hysterothylacium larvae in marine fishes from the East China Sea and the Pacific coast of central Japan.
Authors: Kong, Qingming and Fan, Lanfen and Zhang, Junhe and Akao, Nobuaki and Dong, Kewei and Lou, Di and Ding, Jianzu and Tong, Qunbo and Zheng, Bin and Chen, Rui and Ohta, Nobuo and Lu, Shaohong
Journal: International journal of food microbiology (2015): 1-7

Hybridisation of N4-methylcytosine-containing amplicons on DNA microarrays.
Authors: Flores-Juárez, Cyntia R and Leberre Anton, Véronique and Trévisiol, Emmanuelle and Antaramian, Anaid and González-Jasso, Eva and Pless, Reynaldo C
Journal: Journal of biotechnology (2014): 143-9

LNA-modified isothermal oligonucleotide microarray for differentiating bacilli of similar origin.
Authors: Yan, Jing and Yuan, Ying and Mu, Runqing and Shang, Hong and Guan, Yifu
Journal: Journal of biosciences (2014): 795-804

说明书
dCTP *100 mM PCR Grade* CAS 2056-98-6.pdf

dCTP *100 mM PCR Grade* CAS 2056-98-6 货号17253-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

dCTP *100 mM PCR Grade* CAS 2056-98-6

dCTP *100 mM PCR Grade* CAS 2056-98-6

dCTP *100 mM PCR Grade* CAS 2056-98-6 货号17253 货号 17253 存储条件 在零下15度以下保存, 避免光照
规格 5 mL 价格 8508
Ex (nm) Em (nm)
分子量 467.16 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17253

产品名称：dCTP *100 mM PCR Grade*

CAS：2056-98-6

规格：5ml

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：467.16

外观：液体

溶剂：水

 

产品介绍

2′-脱氧胞苷-5′-三磷酸 (dCTP) 溶液用于 DNA 合成反应，如 PCR、DNA 测序和分子克隆技术。 这种 dCTP溶液适用于需要高质量试剂的许多应用。 此类程序包括逆转录 (RT)、聚合酶链反应 (PCR)、RT-PCR、DNA 标记反应和测序/循环测序分析。

 

参考文献

Enzymatic Synthesis of Chimeric DNA Oligonucleotides by in Vitro Transcription with dTTP, dCTP, dATP, and 2′-Fluoro Modified dGTP.
Authors: Kapustina, Žana and Jasponė, Aistė and Dubovskaja, Varvara and Mackevičius, Gytis and Lubys, Arvydas
Journal: ACS synthetic biology (2021): 1625-1632

Polymerase synthesis of four-base DNA from two stable dimeric nucleotides.
Authors: Mohsen, Michael G and Ji, Debin and Kool, Eric T
Journal: Nucleic acids research (2019): 9495-9501

Self-Priming Enzymatic Fabrication of Multiply Modified DNA.
Authors: Whitfield, Colette J and Little, Rachel C and Khan, Kasid and Ijiro, Kuniharu and Connolly, Bernard A and Tuite, Eimer M and Pike, Andrew R
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2018): 15267-15274

Flexible double-headed cytosine-linked 2′-deoxycytidine nucleotides. Synthesis, polymerase incorporation to DNA and interaction with DNA methyltransferases.
Authors: Kielkowski, Pavel and Cahová, Hana and Pohl, Radek and Hocek, Michal
Journal: Bioorganic & medicinal chemistry (2016): 1268-76

PCR amplification of GC-rich DNA regions using the nucleotide analog N4-methyl-2′-deoxycytidine 5′-triphosphate.
Authors: Flores-Juárez, Cyntia R and González-Jasso, Eva and Antaramian, Anaid and Pless, Reynaldo C
Journal: BioTechniques (2016): 175-182

Fluorescence polarization-based method with bisulfite conversion-specific one-label extension for quantification of single CpG dinucleotide methylation.
Authors: Li, Shufen and Wang, Zhongju and Zhou, Lin and Luo, Fu and Zhao, Cunyou
Journal: Genome (2015): 357-63

Hi-C in Budding Yeast.
Authors: Belton, Jon-Matthew and Dekker, Job
Journal: Cold Spring Harbor protocols (2015): 649-61

Molecular identification of Anisakis and Hysterothylacium larvae in marine fishes from the East China Sea and the Pacific coast of central Japan.
Authors: Kong, Qingming and Fan, Lanfen and Zhang, Junhe and Akao, Nobuaki and Dong, Kewei and Lou, Di and Ding, Jianzu and Tong, Qunbo and Zheng, Bin and Chen, Rui and Ohta, Nobuo and Lu, Shaohong
Journal: International journal of food microbiology (2015): 1-7

Hybridisation of N4-methylcytosine-containing amplicons on DNA microarrays.
Authors: Flores-Juárez, Cyntia R and Leberre Anton, Véronique and Trévisiol, Emmanuelle and Antaramian, Anaid and González-Jasso, Eva and Pless, Reynaldo C
Journal: Journal of biotechnology (2014): 143-9

LNA-modified isothermal oligonucleotide microarray for differentiating bacilli of similar origin.
Authors: Yan, Jing and Yuan, Ying and Mu, Runqing and Shang, Hong and Guan, Yifu
Journal: Journal of biosciences (2014): 795-804

说明书
dCTP *100 mM PCR Grade* CAS 2056-98-6.pdf

5′-三磷酸脱氧胞苷 Aminopropargyl dCTP CAS 2088422-92-6 货号17050-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

5′-三磷酸脱氧胞苷 Aminopropargyl dCTP CAS 2088422-92-6

5′-三磷酸脱氧胞苷 Aminopropargyl dCTP CAS 2088422-92-6

5'-三磷酸脱氧胞苷 Aminopropargyl dCTP CAS 2088422-92-6 货号17050 货号 17050 存储条件 在零下15度以下保存, 避免光照
规格 10 umoles 价格 6564
Ex (nm) Em (nm)
分子量 543.95 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17050

产品名称：5′-三磷酸脱氧胞苷 Aminopropargyl dCTP

CAS：2088422-92-6

规格：10umoles

储存条件：-15℃避光干燥

保质期：12个月

 

产品物理化学光谱特性

分子量：608.15

溶剂：水

激发波长(nm)：N/A

发射波长(nm)：N/A

 

产品介绍

胺修饰的脱氧胞苷5′-三磷酸酯（例如5-炔丙基氨基-2′-脱氧胞苷5′-三磷酸酯）可用于通过常规酶促掺入方法（如反转录，缺口翻译，随机引物）产生含胺的DNA。标记或PCR。氨基炔丙基dCTP可以通过常规酶结合技术容易地结合到DNA中。然后可以使用任何胺反应性荧光染料，生物素和其他胺反应性试剂对所得的胺修饰的核酸进行标记。与通常具有较高立体阻碍的标签标记核苷酸相比，氨基炔丙基修饰的核苷酸可以掺入到极高且一致的水平。胺修饰的核酸与过量的胺反应性试剂的随后反应，无论选择何种标记试剂，均能实现相应较高的一致标记效率。这种两步标记方法还消除了优化酶促反应以适应不同染料修饰核苷酸的需要，该修饰可能以非常不同的速率掺入。这种标记方法广泛用于FISH探针和基于微孔板的实验。

 

参考文献

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919

Fluorescent labelling of cRNA for microarray applications
Authors: t Hoen PA, de Kort F, van Ommen GJ, den Dunnen JT.
Journal: Nucleic Acids Res (2003): e20

Sequence-specific scission of DNA by the chemical nuclease activity of 1,10-phenanthroline-copper(I) targeted by RNA
Authors: Chen CB, Gorin MB, Sigman DS.
Journal: Proc Natl Acad Sci U S A (1993): 4206

说明书
5′-三磷酸脱氧胞苷 Aminopropargyl dCTP CAS 2088422-92-6.pdf

dCTP *100 mM PCR Grade* CAS 2056-98-6 货号17252-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

dCTP *100 mM PCR Grade* CAS 2056-98-6

dCTP *100 mM PCR Grade* CAS 2056-98-6

dCTP *100 mM PCR Grade* CAS 2056-98-6 货号17252 货号 17252 存储条件 在零下15度以下保存, 避免光照
规格 500 uL 价格 1164
Ex (nm) Em (nm)
分子量 467.16 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17252

产品名称：dCTP *100 mM PCR Grade*

CAS：2056-98-6

规格：500ul

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：467.16

外观：液体

溶剂：水

 

产品介绍

2′-脱氧胞苷-5′-三磷酸 (dCTP) 溶液用于 DNA 合成反应，如 PCR、DNA 测序和分子克隆技术。 这种 dCTP溶液适用于需要高质量试剂的许多应用。 此类程序包括逆转录 (RT)、聚合酶链反应 (PCR)、RT-PCR、DNA 标记反应和测序/循环测序分析。

 

参考文献

Enzymatic Synthesis of Chimeric DNA Oligonucleotides by in Vitro Transcription with dTTP, dCTP, dATP, and 2′-Fluoro Modified dGTP.
Authors: Kapustina, Žana and Jasponė, Aistė and Dubovskaja, Varvara and Mackevičius, Gytis and Lubys, Arvydas
Journal: ACS synthetic biology (2021): 1625-1632

Polymerase synthesis of four-base DNA from two stable dimeric nucleotides.
Authors: Mohsen, Michael G and Ji, Debin and Kool, Eric T
Journal: Nucleic acids research (2019): 9495-9501

Self-Priming Enzymatic Fabrication of Multiply Modified DNA.
Authors: Whitfield, Colette J and Little, Rachel C and Khan, Kasid and Ijiro, Kuniharu and Connolly, Bernard A and Tuite, Eimer M and Pike, Andrew R
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2018): 15267-15274

Flexible double-headed cytosine-linked 2′-deoxycytidine nucleotides. Synthesis, polymerase incorporation to DNA and interaction with DNA methyltransferases.
Authors: Kielkowski, Pavel and Cahová, Hana and Pohl, Radek and Hocek, Michal
Journal: Bioorganic & medicinal chemistry (2016): 1268-76

PCR amplification of GC-rich DNA regions using the nucleotide analog N4-methyl-2′-deoxycytidine 5′-triphosphate.
Authors: Flores-Juárez, Cyntia R and González-Jasso, Eva and Antaramian, Anaid and Pless, Reynaldo C
Journal: BioTechniques (2016): 175-182

Fluorescence polarization-based method with bisulfite conversion-specific one-label extension for quantification of single CpG dinucleotide methylation.
Authors: Li, Shufen and Wang, Zhongju and Zhou, Lin and Luo, Fu and Zhao, Cunyou
Journal: Genome (2015): 357-63

Hi-C in Budding Yeast.
Authors: Belton, Jon-Matthew and Dekker, Job
Journal: Cold Spring Harbor protocols (2015): 649-61

Molecular identification of Anisakis and Hysterothylacium larvae in marine fishes from the East China Sea and the Pacific coast of central Japan.
Authors: Kong, Qingming and Fan, Lanfen and Zhang, Junhe and Akao, Nobuaki and Dong, Kewei and Lou, Di and Ding, Jianzu and Tong, Qunbo and Zheng, Bin and Chen, Rui and Ohta, Nobuo and Lu, Shaohong
Journal: International journal of food microbiology (2015): 1-7

Hybridisation of N4-methylcytosine-containing amplicons on DNA microarrays.
Authors: Flores-Juárez, Cyntia R and Leberre Anton, Véronique and Trévisiol, Emmanuelle and Antaramian, Anaid and González-Jasso, Eva and Pless, Reynaldo C
Journal: Journal of biotechnology (2014): 143-9

LNA-modified isothermal oligonucleotide microarray for differentiating bacilli of similar origin.
Authors: Yan, Jing and Yuan, Ying and Mu, Runqing and Shang, Hong and Guan, Yifu
Journal: Journal of biosciences (2014): 795-804

说明书
dCTP *100 mM PCR Grade* CAS 2056-98-6.pdf

5-Propargylamino-3′-azidomethyl-dCTP 货号17091-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

5-Propargylamino-3′-azidomethyl-dCTP

5-Propargylamino-3′-azidomethyl-dCTP

5-Propargylamino-3'-azidomethyl-dCTP 货号17091 货号 17091 存储条件 在零下15度以下保存, 避免光照
规格 50 nmoles 价格 3108
Ex (nm) Em (nm)
分子量 575.26 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17091

产品名称：5-Propargylamino-3′-azidomethyl-dCTP

规格：50 nmoles

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：575.26

溶剂：水

 

产品介绍

5-Propargylamino-3′-azidomethyl-dCTP是制备用于下一代测序（NGS）的荧光缀合物的关键组成部分。 NGS使用与早期Sanger测序相似的链终止方法，但NGS是通过荧光标记的核苷酸类似物作为扩增反应的可逆终止剂来进行的。 NGS依赖于可逆的DNA聚合阻断，而Sanger测序则使用ddNTP不可逆转的DNA聚合阻断。 NGS的另一个不同特征是通过桥式PCR进行体外克隆扩增以增加待测序分子的数量。在此平台上，片段与固定在固体表面上的引物连接，进行原位扩增，生成具有相同分子的DNA簇。在每个循环中，同时添加可逆终止的四个核苷酸，并通过它们互补的聚合酶掺入。通过将3′-OH基团替换为3′-o-叠氮基甲基，这些核苷酸被化学封闭，以防止聚合酶在每个循环中掺入一个以上的核苷酸。掺入核苷酸后，在不同通道中针对不同碱基测量荧光信号。关于下一循环，洗涤未掺入的核苷酸，并用TCEP去除3’端的化学封锁。一旦收集到荧光信号，就会开始一个新的循环，重复此动态过程，直到完成每个片段的测序为止。总之，NGS测序反应分三个步骤进行：核苷酸的添加，成像和通过荧光团裂解的3′-OH再生。

 

参考文献

Genome-wide analysis of the specificity and mechanisms of replication infidelity driven by imbalanced dNTP pools.
Authors: Watt, Danielle L and Buckland, Robert J and Lujan, Scott A and Kunkel, Thomas A and Chabes, Andrei
Journal: Nucleic acids research (2016): 1669-80

Fluorescence polarization-based method with bisulfite conversion-specific one-label extension for quantification of single CpG dinucleotide methylation.
Authors: Li, Shufen and Wang, Zhongju and Zhou, Lin and Luo, Fu and Zhao, Cunyou
Journal: Genome (2015): 357-63

Hi-C in Budding Yeast.
Authors: Belton, Jon-Matthew and Dekker, Job
Journal: Cold Spring Harbor protocols (2015): 649-61

Transcriptome analysis of Capsicum annuum varieties Mandarin and Blackcluster: assembly, annotation and molecular marker discovery.
Authors: Ahn, Yul-Kyun and Tripathi, Swati and Kim, Jeong-Ho and Cho, Young-Il and Lee, Hye-Eun and Kim, Do-Sun and Woo, Jong-Gyu and Cho, Myeong-Cheoul
Journal: Gene (2014): 494-9

Structures of an apo and a binary complex of an evolved archeal B family DNA polymerase capable of synthesising highly cy-dye labelled DNA.
Authors: Wynne, Samantha A and Pinheiro, Vitor B and Holliger, Philipp and Leslie, Andrew G W
Journal: PloS one (2013): e70892

说明书
5-Propargylamino-3′-azidomethyl-dCTP.pdf