核紫 DCS1 死细胞染料
|
货号 |
17549 |
存储条件 |
在零下15度以下保存, 避免光照 |
规格 |
0.5 ml |
价格 |
1164 |
Ex (nm) |
371 |
Em (nm) |
454 |
分子量 |
744.77 |
溶剂 |
DMSO |
产品详细介绍 |
简要概述
产品基本信息
货号:17549
产品名称:核紫 DCS1 死细胞染料
规格:0.5ml
储存条件:-20℃干燥
产品物理化学光谱特性
溶剂:DMSO
激发波长(nm):371
发射波长(nm):454
产品介绍
我们的Nuclear Violet DCS1是一种非荧光,DNA选择性和细胞不透性的紫色荧光染料,用于分析死细胞中的DNA含量。 核紫色DCS1在与双链DNA结合后具有明显增强的荧光。 它可用于荧光成像,微孔板和流式细胞仪应用。 这种DNA结合染料可用于死细胞的多色分析。
点击查看光谱
适用仪器
荧光显微镜 |
|
激发: |
DAPI |
发射: |
DAPI |
推荐孔板: |
黑色透明孔板 |
产品说明书
染色细胞分析方案
注意:以下方案适用于大多数细胞类型。 生长培养基,细胞密度,其他细胞类型和因子的存在可能影响染色。 玻璃器皿上的残留洗涤剂也可能影响许多生物的染色,并导致在有或没有细胞存在的溶液中出现明亮染色的物质。
工作溶液配制
使用缓冲液将核紫 DCS1储备溶液(5mM)稀释到0.5-5μm的浓度。
操作步骤
1.将Nuclear Violet DCS1工作溶液加到固定、死亡或凋亡的细胞(悬浮液或粘附细胞)中,并将细胞染色15至60分钟。 在最初的实验中,最好尝试几种染料浓度来确定产生所需结果的最佳浓度。 高染料浓度倾向于引起其他细胞结构的非特异性染色。
2.用荧光显微镜、荧光酶标仪或流式细胞仪直接检测染色细胞。
图示
图1.在96孔板上染色的固定和活(非固定)Hela细胞,与核蓝 DCS1 1μm孵育20分钟并在DAPI通道成像。
参考文献
Assessment of laser-induced thermal damage in fresh skin with ex vivo confocal microscopy.
Authors: Ortner, Vinzent Kevin and Sahu, Aditi and Haedersdal, Merete and Rajadhyaksha, Milind and Rossi, Anthony Mario
Journal: Journal of the American Academy of Dermatology (2021): e19-e21
Development of a novel flow cytometry-based approach for reticulocytes micronucleus test in rat peripheral blood.
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Exploring the utility of Deep Red Anthraquinone 5 for digital staining of ex vivo confocal micrographs of optically sectioned skin.
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Journal: Journal of biophotonics (2021): e202000207
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Journal: Cytometry. Part B, Clinical cytometry (2020): 399-411
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Journal: Cancers (2020)
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Journal: Molecules (Basel, Switzerland) (2020)
TEM observation of compacted DNA of Synechococcus elongatus PCC 7942 using DRAQ5 labelling with DAB photooxidation and osmium black.
Authors: Ghosh, Ilika and Atsuzawa, Kimie and Arai, Aoi and Ohmukai, Ryuzo and Kaneko, Yasuko
Journal: Microscopy (Oxford, England) (2020)
[The Establishment of a Three-color Flow Cytometry Approach for the Scoring of Micronucleated Reticulocytes in Rat Bone Marrow].
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Automated Triage Radiation Biodosimetry: Integrating Imaging Flow Cytometry with High-Throughput Robotics to Perform the Cytokinesis-Block Micronucleus Assay.
Authors: Wang, Qi and Rodrigues, Matthew A and Repin, Mikhail and Pampou, Sergey and Beaton-Green, Lindsay A and Perrier, Jay and Garty, Guy and Brenner, David J and Turner, Helen C and Wilkins, Ruth C
Journal: Radiation research (2019): 342-351
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