标签归档：datp

Cy5-dATP 货号17038-AAT Bioquest荧光染料

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上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Cy5-dATP

Cy5-dATP

Cy5-dATP 货号17038 货号 17038 存储条件 在零下15度以下保存, 避免光照
规格 25 nmoles 价格 1200
Ex (nm) 651 Em (nm) 670
分子量 1182.05 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17038

产品名称：Cy5-dATP

规格：25 nmoles

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：1182.05

溶剂：水

激发波长(nm)：651

发射波长(nm)：670

消光系数（cm-1 M-1）:250000

荧光量子产率：0.27（PBS）,0.4(乙醇)

校正因子 (280 nm)：0.03

 

产品介绍

染料修饰的脱氧腺苷5′-三磷酸酯广泛用于各种非放射性DNA标记反应，包括切口平移，随机引物标记，cDNA标记和3′-末端标记。 Cy5-dATP可用于通过常规酶促掺入方法（例如逆转录，缺口翻译，随机引物标记或PCR）产生含Cy5的DNA。这种酶促荧光标记方法广泛用于FISH探针和基于微阵列的实验。此Cy5-dATP缀合物可与SpectrumFRed 滤波片组一起用作红色荧光色（SpectrumFRed 是Vysis的商标）。它在功能上等同于Cy5-dATP（珀金·埃尔默（Perkin Elmer）的NEL593001EA），稳定性大大提高。

点击查看光谱

 

参考文献

2-Formyl-dATP as Substrate for Polymerase Synthesis of Reactive DNA Bearing an Aldehyde Group in the Minor Groove.
Authors: Krömer, Matouš and Brunderová, Mária and Ivancová, Ivana and Poštová Slavětínská, Lenka and Hocek, Michal
Journal: ChemPlusChem (2020): 1164-1170

Fluorescent dATP for DNA Synthesis In Vivo.
Authors: Schreier, Verena N and Loehr, Morten O and Deng, Ting and Lattmann, Evelyn and Hajnal, Alex and Neuhauss, Stephan C F and Luedtke, Nathan W
Journal: ACS chemical biology (2020): 2996-3003

New bioluminescence pyrophosphate assay for high-sensitivity detection of food-borne pathogens.
Authors: Fei, Zhongjie and Zhou, Dongrui and Li, Na and Xiao, Pengfeng
Journal: Luminescence : the journal of biological and chemical luminescence (2020): 355-364

Binding studies of a putative C. pseudotuberculosis target protein from Vitamin B12 Metabolism.
Authors: Peinado, Rafaela Dos S and Olivier, Danilo S and Eberle, Raphael J and de Moraes, Fabio R and Amaral, Marcos S and Arni, Raghuvir K and Coronado, Monika A
Journal: Scientific reports (2019): 6350

ELTA: Enzymatic Labeling of Terminal ADP-Ribose.
Authors: Ando, Yoshinari and Elkayam, Elad and McPherson, Robert Lyle and Dasovich, Morgan and Cheng, Shang-Jung and Voorneveld, Jim and Filippov, Dmitri V and Ong, Shao-En and Joshua-Tor, Leemor and Leung, Anthony K L
Journal: Molecular cell (2019): 845-856.e5

Label-Free Telomerase Detection in Single Cell Using a Five-Base Telomerase Product-Triggered Exponential Rolling Circle Amplification Strategy.
Authors: Li, XiaoYu and Cui, YunXi and Du, YiChen and Tang, AnNa and Kong, DeMing
Journal: ACS sensors (2019): 1090-1096

Terminal deoxynucleotidyl transferase-initiated molecule beacons arrayed aptamer probe for sensitive detection of metastatic colorectal cancer cells.
Authors: Zhao, Yujie and Ma, Wenjie and Zou, Shanzi and Chen, Biao and Cheng, Hong and He, Xiaoxiao and Wang, Kemin
Journal: Talanta (2019): 152-158

2-Allyl- and Propargylamino-dATPs for Site-Specific Enzymatic Introduction of a Single Modification in the Minor Groove of DNA.
Authors: Matyašovský, Ján and Pohl, Radek and Hocek, Michal
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2018): 14938-14941

DNA Primer Extension with Cyclopropenylated 7-Deaza-2′-deoxyadenosine and Efficient Bioorthogonal Labeling in Vitro and in Living Cells.
Authors: Ploschik, Damian and Rönicke, Franziska and Beike, Hanna and Strasser, Ralf and Wagenknecht, Hans-Achim
Journal: Chembiochem : a European journal of chemical biology (2018): 1949-1953

Gp41, a superfamily SF2 helicase from bacteriophage BFK20.
Authors: Halgasova, Nora and Matuskova, Radka and Kraus, Daniel and Tkacova, Adela and Balusikova, Lenka and Bukovska, Gabriela
Journal: Virus research (2018): 7-16

说明书
Cy5-dATP.pdf

dATP *100 mM PCR Grade* 货号17250-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

dATP *100 mM PCR Grade*

dATP *100 mM PCR Grade*

dATP *100 mM PCR Grade* 货号17250 货号 17250 存储条件 在零下15度以下保存, 避免光照
规格 500 uL 价格 1164
Ex (nm) Em (nm)
分子量 491.18 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17250

产品名称：dATP *100 mM PCR Grade*

CAS：1927-31-7

规格：500ul

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：491.18

外观：液体

溶剂：水

 

产品介绍

2′-脱氧腺苷 5′-三磷酸钠盐 (dATP) 溶液用于 DNA 合成反应，如 PCR、DNA 测序和分子克隆技术。 这种 dATP 溶液适用于需要高质量试剂的许多应用。 此类程序包括逆转录 (RT)、聚合酶链反应 (PCR)、RT-PCR、DNA 标记反应和测序/循环测序分析。

 

参考文献

Development of a Novel Bioluminescence Pyrophosphate Assay for the High-Sensitivity Detection of Hepatitis B Virus.
Authors: Tan, Guolei and Fei, Zhongjie and Wei, Rongbin and Wu, Xuping and Xiao, Pengfeng
Journal: Applied biochemistry and biotechnology (2021)

Enzymatic Synthesis of Chimeric DNA Oligonucleotides by in Vitro Transcription with dTTP, dCTP, dATP, and 2′-Fluoro Modified dGTP.
Authors: Kapustina, Žana and Jasponė, Aistė and Dubovskaja, Varvara and Mackevičius, Gytis and Lubys, Arvydas
Journal: ACS synthetic biology (2021): 1625-1632

New bioluminescence pyrophosphate assay for high-sensitivity detection of food-borne pathogens.
Authors: Fei, Zhongjie and Zhou, Dongrui and Li, Na and Xiao, Pengfeng
Journal: Luminescence : the journal of biological and chemical luminescence (2020): 355-364

Plasmodium falciparum DDX55 is a nucleocytoplasmic protein and a 3′-5′ direction-specific DNA helicase.
Authors: Yasmin, Rahena and Kaur, Inderjeet and Tuteja, Renu
Journal: Protoplasma (2020): 1049-1067

Duplex Electrochemical DNA Sensor to Detect Bacillus anthracis CAP and PAG DNA Targets Based on the Incorporation of Tailed Primers and Ferrocene-Labeled dATP.
Authors: Magriñá, Ivan and Jauset-Rubio, Miriam and Ortiz, Mayreli and Tomaso, Herbert and Simonova, Anna and Hocek, Michal and O’Sullivan, Ciara K
Journal: ACS omega (2019): 21900-21908

Electrochemical genosensor for the direct detection of tailed PCR amplicons incorporating ferrocene labelled dATP.
Authors: Magriñá, Ivan and Toldrà, Anna and Campàs, Mònica and Ortiz, Mayreli and Simonova, Anna and Katakis, Ioanis and Hocek, Michal and O’Sullivan, Ciara K
Journal: Biosensors & bioelectronics (2019): 76-82

Polymerase synthesis of four-base DNA from two stable dimeric nucleotides.
Authors: Mohsen, Michael G and Ji, Debin and Kool, Eric T
Journal: Nucleic acids research (2019): 9495-9501

Self-Priming Enzymatic Fabrication of Multiply Modified DNA.
Authors: Whitfield, Colette J and Little, Rachel C and Khan, Kasid and Ijiro, Kuniharu and Connolly, Bernard A and Tuite, Eimer M and Pike, Andrew R
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2018): 15267-15274

Alr2954 of Anabaena sp. PCC 7120 with ADP-ribose pyrophosphatase activity bestows abiotic stress tolerance in Escherichia coli.
Authors: Singh, Prashant Kumar and Shrivastava, Alok Kumar and Singh, Shilpi and Rai, Ruchi and Chatterjee, Antra and Rai, L C
Journal: Functional & integrative genomics (2017): 39-52

GATA4 Is Sufficient to Establish Jejunal Versus Ileal Identity in the Small Intestine.
Authors: Thompson, Cayla A and Wojta, Kevin and Pulakanti, Kirthi and Rao, Sridhar and Dawson, Paul and Battle, Michele A
Journal: Cellular and molecular gastroenterology and hepatology (2017): 422-446

说明书
dATP *100 mM PCR Grade*.pdf

脱氧腺嘌呤5′-三磷酸酯 Aminopropargyl dATP CAS 587848-72-4 货号17056-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

脱氧腺嘌呤5′-三磷酸酯 Aminopropargyl dATP CAS 587848-72-4

脱氧腺嘌呤5′-三磷酸酯 Aminopropargyl dATP CAS 587848-72-4

脱氧腺嘌呤5'-三磷酸酯 Aminopropargyl dATP CAS 587848-72-4 货号17056 货号 17056 存储条件 在零下15度以下保存, 避免光照
规格 10 umoles 价格 7512
Ex (nm) Em (nm)
分子量 566.99 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17056

产品名称：氨基炔丙基dATP[7-脱氮-7-炔丙基氨基-2′-脱氧腺苷-5′-三磷酸]

CAS：587848-72-4

规格：10 umoles

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：631.18

外观：无色

溶剂：水

 

产品介绍

氨基炔丙基dATP[7-脱氮-7-炔丙基氨基-2′-脱氧腺苷-5′-三磷酸]是美国AAT Bioquest生产的核苷酸，胺修饰的脱氧腺嘌呤5′-三磷酸（如5-炔丙基氨基-2′-脱氧腺嘌呤-5′-三磷酸）可用于通过常规的酶促掺入方法产生含胺的DNA，例如逆转录，切口平移，随机引物标记或PCR。氨基炔丙基dATP可以通过常规的酶促掺入技术容易地掺入DNA中。然后可以使用胺反应性荧光染料，生物素和其他胺反应性试剂中的任何一种来标记所得的胺修饰的核酸。与通常具有较高立体异构的标签标记的核苷酸相比，氨基炔丙基修饰的核苷酸可以掺入极高且一致的水平。无论选择何种标记试剂，胺修饰的核酸随后与过量的胺反应性试剂的反应实现相应的高且一致的标记效率。这种两步标记方法还消除了优化酶促反应以适应不同染料修饰的核苷酸的需要，其可以以非常不同的速率掺入。该标记方法广泛用于FISH探针和基于微阵列的实验。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的氨基炔丙基dATP[7-脱氮-7-炔丙基氨基-2′-脱氧腺苷-5′-三磷酸]。 

 

参考文献

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919

Fluorescent labelling of cRNA for microarray applications
Authors: t Hoen PA, de Kort F, van Ommen GJ, den Dunnen JT.
Journal: Nucleic Acids Res (2003): e20

Sequence-specific scission of DNA by the chemical nuclease activity of 1,10-phenanthroline-copper(I) targeted by RNA
Authors: Chen CB, Gorin MB, Sigman DS.
Journal: Proc Natl Acad Sci U S A (1993): 4206

说明书
脱氧腺嘌呤5′-三磷酸酯 Aminopropargyl dATP CAS 587848-72-4.pdf

dATP *100 mM PCR Grade* 货号17250-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

dATP *100 mM PCR Grade*

dATP *100 mM PCR Grade*

dATP *100 mM PCR Grade* 货号17250 货号 17250 存储条件 在零下15度以下保存, 避免光照
规格 500 uL 价格 1164
Ex (nm) Em (nm)
分子量 491.18 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17250

产品名称：dATP *100 mM PCR Grade*

CAS：1927-31-7

规格：500ul

储存条件：-15℃避光防潮

保质期：12个月

 

产品物理化学光谱特性

分子量：491.18

外观：液体

溶剂：水

 

产品介绍

2′-脱氧腺苷 5′-三磷酸钠盐 (dATP) 溶液用于 DNA 合成反应，如 PCR、DNA 测序和分子克隆技术。 这种 dATP 溶液适用于需要高质量试剂的许多应用。 此类程序包括逆转录 (RT)、聚合酶链反应 (PCR)、RT-PCR、DNA 标记反应和测序/循环测序分析。

 

参考文献

Development of a Novel Bioluminescence Pyrophosphate Assay for the High-Sensitivity Detection of Hepatitis B Virus.
Authors: Tan, Guolei and Fei, Zhongjie and Wei, Rongbin and Wu, Xuping and Xiao, Pengfeng
Journal: Applied biochemistry and biotechnology (2021)

Enzymatic Synthesis of Chimeric DNA Oligonucleotides by in Vitro Transcription with dTTP, dCTP, dATP, and 2′-Fluoro Modified dGTP.
Authors: Kapustina, Žana and Jasponė, Aistė and Dubovskaja, Varvara and Mackevičius, Gytis and Lubys, Arvydas
Journal: ACS synthetic biology (2021): 1625-1632

New bioluminescence pyrophosphate assay for high-sensitivity detection of food-borne pathogens.
Authors: Fei, Zhongjie and Zhou, Dongrui and Li, Na and Xiao, Pengfeng
Journal: Luminescence : the journal of biological and chemical luminescence (2020): 355-364

Plasmodium falciparum DDX55 is a nucleocytoplasmic protein and a 3′-5′ direction-specific DNA helicase.
Authors: Yasmin, Rahena and Kaur, Inderjeet and Tuteja, Renu
Journal: Protoplasma (2020): 1049-1067

Duplex Electrochemical DNA Sensor to Detect Bacillus anthracis CAP and PAG DNA Targets Based on the Incorporation of Tailed Primers and Ferrocene-Labeled dATP.
Authors: Magriñá, Ivan and Jauset-Rubio, Miriam and Ortiz, Mayreli and Tomaso, Herbert and Simonova, Anna and Hocek, Michal and O’Sullivan, Ciara K
Journal: ACS omega (2019): 21900-21908

Electrochemical genosensor for the direct detection of tailed PCR amplicons incorporating ferrocene labelled dATP.
Authors: Magriñá, Ivan and Toldrà, Anna and Campàs, Mònica and Ortiz, Mayreli and Simonova, Anna and Katakis, Ioanis and Hocek, Michal and O’Sullivan, Ciara K
Journal: Biosensors & bioelectronics (2019): 76-82

Polymerase synthesis of four-base DNA from two stable dimeric nucleotides.
Authors: Mohsen, Michael G and Ji, Debin and Kool, Eric T
Journal: Nucleic acids research (2019): 9495-9501

Self-Priming Enzymatic Fabrication of Multiply Modified DNA.
Authors: Whitfield, Colette J and Little, Rachel C and Khan, Kasid and Ijiro, Kuniharu and Connolly, Bernard A and Tuite, Eimer M and Pike, Andrew R
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2018): 15267-15274

Alr2954 of Anabaena sp. PCC 7120 with ADP-ribose pyrophosphatase activity bestows abiotic stress tolerance in Escherichia coli.
Authors: Singh, Prashant Kumar and Shrivastava, Alok Kumar and Singh, Shilpi and Rai, Ruchi and Chatterjee, Antra and Rai, L C
Journal: Functional & integrative genomics (2017): 39-52

GATA4 Is Sufficient to Establish Jejunal Versus Ileal Identity in the Small Intestine.
Authors: Thompson, Cayla A and Wojta, Kevin and Pulakanti, Kirthi and Rao, Sridhar and Dawson, Paul and Battle, Michele A
Journal: Cellular and molecular gastroenterology and hepatology (2017): 422-446

说明书
dATP *100 mM PCR Grade*.pdf

生物素-11-dATP 货号17014-AAT Bioquest荧光染料

发表于由

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。

生物素-11-dATP

生物素-11-dATP

生物素-11-dATP 货号17014 货号 17014 存储条件 在零下15度以下保存, 避免光照
规格 25 nmoles 价格 1200
Ex (nm) Em (nm)
分子量 882.71 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号：17014

产品名称：生物素-11-dATP

规格：25 nmoles

储存条件：-15℃避光防潮

保质期：24个月

 

产品物理化学光谱特性

分子量：882.71

溶剂：水

 

产品介绍

生物素修饰的脱氧腺苷5′-三磷酸酯广泛用于各种非放射性DNA标记反应，包括切口平移，随机引物标记，cDNA标记和3′-末端标记。生物素-11-dATP可通过切口平移，随机引物，3′-末端末端标记或在PCR过程中酶促地掺入DNA中。数字“ 11”是dATP和生物素之间连接子主链中的碳原子数。连接子越长，生物素与抗生物素蛋白的相互作用越有效。另一方面，连接子越短，dATP掺入DNA的效率就越高。建议链接器’11’的长度对于大多数应用而言是最佳的。 Biotin-11-dATP用于在各种杂交应用中产生生物素化的DNA探针，包括Southern印迹，Northern印迹，斑点印迹，固定细胞和组织。它在化学上等同于PerkinElmer（PE）的NEL540001EA。

 

参考文献

Measurement of differential chromatin interactions with absolute quantification of architecture (AQuA-HiChIP).
Authors: Gryder, Berkley E and Khan, Javed and Stanton, Benjamin Z
Journal: Nature protocols (2020): 1209-1236

Highly sensitive and selective electrochemical detection of Hg(2+) through surface-initiated enzymatic polymerization.
Authors: Mei, Chenyang and Lin, Dajie and Fan, Chengchao and Liu, Aili and Wang, Shun and Wang, Jichang
Journal: Biosensors & bioelectronics (2016): 105-110

Ultrasensitive electrochemical DNA sensor based on the target induced structural switching and surface-initiated enzymatic polymerization.
Authors: Wan, Ying and Wang, Pengjuan and Su, Yan and Zhu, Xinhua and Yang, Shulin and Lu, Jianxin and Gao, Jimin and Fan, Chunhai and Huang, Qing
Journal: Biosensors & bioelectronics (2014): 231-6

A surface-initiated enzymatic polymerization strategy for electrochemical DNA sensors.
Authors: Wan, Ying and Xu, Hui and Su, Yan and Zhu, Xinhua and Song, Shiping and Fan, Chunhai
Journal: Biosensors & bioelectronics (2013): 526-31

Oxidative DNA injury after experimental intracerebral hemorrhage.
Authors: Nakamura, Takehiro and Keep, Richard F and Hua, Ya and Hoff, Julian T and Xi, Guohua
Journal: Brain research (2005): 30-6

Impact of hypoglycemia and diabetes on CNS: correlation of mitochondrial oxidative stress with DNA damage.
Authors: Singh, Puneet and Jain, Anu and Kaur, Gurcharan
Journal: Molecular and cellular biochemistry (2004): 153-9

Oxidative brain injury from extravasated erythrocytes after intracerebral hemorrhage.
Authors: Wu, Jimin and Hua, Ya and Keep, Richard F and Schallert, Timothy and Hoff, Julian T and Xi, Guohua
Journal: Brain research (2002): 45-52

Detection of single- and double-strand DNA breaks after traumatic brain injury in rats: comparison of in situ labeling techniques using DNA polymerase I, the Klenow fragment of DNA polymerase I, and terminal deoxynucleotidyl transferase.
Authors: Clark RSB and Chen, M and Kochanek, P M and Watkins, S C and Jin, K L and Draviam, R and Nathaniel, P D and Pinto, R and Marion, D W and Graham, S H
Journal: Journal of neurotrauma (2001): 675-89

Activation of poly(ADP-ribose) polymerase in the rat hippocampus may contribute to cellular recovery following sublethal transient global ischemia.
Authors: Nagayama, T and Simon, R P and Chen, D and Henshall, D C and Pei, W and Stetler, R A and Chen, J
Journal: Journal of neurochemistry (2000): 1636-45

Formation of the base modification 8-hydroxyl-2′-deoxyguanosine and DNA fragmentation following seizures induced by systemic kainic acid in the rat.
Authors: Lan, J and Henshall, D C and Simon, R P and Chen, J
Journal: Journal of neurochemistry (2000): 302-9

说明书
生物素-11-dATP.pdf