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DAPI乳酸 CAS 28718-91-4
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货号 | 17509 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 25 mg | 价格 | 1272 | |
| Ex (nm) | 359 | Em (nm) | 457 | |
| 分子量 | 457.48 | 溶剂 | Water | |
| 产品详细介绍 | ||||
简要概述
产品基本信息
货号：17509
产品名称：DAPI乳酸
CAS：28718-91-4
规格：25mg
储存条件：保存在冰箱-20℃干燥
保质期：6个月
产品物理化学光谱特性
分子量：457.48
溶剂：水
Ex(nm):358
Em(nm):461
产品介绍
DAPI是一种与DNA结合强烈的荧光染色剂。它广泛用于荧光显微镜。由于DAPI通过完整的细胞膜，它可用于除固定细胞外的活细胞染色。对于荧光显微镜，DAPI被紫外线激发。当与双链DNA结合时，其最大吸收在358nm，其发射最大值在461nm。DAPI的一个缺点是其排放相当广泛。DAPI也与RNA结合，但是它不像它与DNA结合时那样强烈地发荧光。 当与RNA结合时，其发射偏移至约500nm .DAPI的蓝色发射便于多重测定，因为DAPI与荧光素和绿色荧光蛋白（GFP）等绿色荧光分子或红色荧光染料之间的荧光重叠非常少。除了标记细胞核外，DAPI还用于检测细胞培养物中的支原体或病毒DNA。
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参考文献
A low dose of ethidium bromide leads to an increase of total mitochondrial DNA while higher concentrations induce the mtDNA 4997 deletion in a human neuronal cell line
Authors: von Wurmb-Schwark N, Cavelier L, Cortopassi GA.
Journal: Mutat Res (2006): 57
Acetylcholinesterase activity in rats experimentally demyelinated with ethidium bromide and treated with interferon beta
Authors: Mazzanti CM, Spanevello RM, Pereira LB, Goncalves JF, Kaizer R, Correa M, Ahmed M, Mazzanti A, Festugatto R, Graca DL, Morsch VM, Schetinger MR.
Journal: Neurochem Res (2006): 1027
Analysis of cycloheximide-induced apoptosis in human leukocytes: Fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide
Authors: Baskic D, Popovic S, Ristic P, Arsenijevic NN.
Journal: Cell Biol Int (2006): 924
Apyrase and 5′-nucleotidase activities in synaptosomes from the cerebral cortex of rats experimentally demyelinated with ethidium bromide and treated with interferon-beta
Authors: Spanevello RM, Mazzanti CM, Kaizer R, Zanin R, Cargnelutti D, Hannel L, Correa M, Mazzanti A, Festugatto R, Graca D, Schetinger MR, Morsch VM.
Journal: Neurochem Res (2006): 455
Atomic force microscopic examination of chromosomes treated with trypsin or ethidium bromide
Authors: Wu Y, Cai J, Cheng L, Yun K, Wang C, Chen Y.
Journal: Chem Pharm Bull (Tokyo) (2006): 501
Delayed Schwann cell and oligodendrocyte remyelination after ethidium bromide injection in the brainstem of Wistar rats submitted to streptozotocin diabetogenic treatment
Authors: Bondan EF, Lallo MA, Trigueiro AH, Ribeiro CP, Sinhorini IL, Graca DL.
Journal: Braz J Med Biol Res (2006): 637
Determination of the DNA-binding characteristics of ethidium bromide, proflavine, and cisplatin by flow injection analysis: usefulness in studies on antitumor drugs
Authors: Alonso A, Almendral MJ, Curto Y, Criado JJ, Rodriguez E, Manzano JL.
Journal: Anal Biochem (2006): 157
Differential pulse voltammetric studies of ethidium bromide binding to DNA
Authors: Minasyan SH, Tavadyan LA, Antonyan AP, Davtyan HG, Parsadanyan MA, Vardevanyan PO.
Journal: Bioelectrochemistry (2006): 48
Ethidium bromide inhibits rat brain acetylcholinesterase activity in vitro
Authors: Mazzanti CM, Spanevello RM, Obregon A, Pereira LB, Streher CA, Ahmed M, Mazzanti A, Graca DL, Morsch VM, Schetinger MR.
Journal: Chem Biol Interact (2006): 121
Ethidium bromide is good not only for staining of nucleic acids but also for staining of proteins after polyacrylamide gel soaking in trichloroacetic acid solution
Authors: Dvortsov IA, Lunina NA, Chekanovskaya LA, Shedova EN, Gening LV, Velikodvorskaya GA.
Journal: Anal Biochem (2006): 293
