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DAPI(4′,6-二脒基-2-苯基吲哚二盐酸盐)*10mM水溶液* CAS 28718-90-3
货号 | 17507 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 2 mL | 价格 | 1008 | |
Ex (nm) | 359 | Em (nm) | 457 | |
分子量 | 350.25 | 溶剂 | Water | |
产品详细介绍 |
简要概述
产品基本信息
货号:17507
产品名称:DAPI[4,6-二脒基-2-苯基吲哚,二盐酸盐]* 10 mM水溶液*
CAS:28718-90-3
规格:2ml
储存条件:保存在冰箱-20℃干燥
保质期:6个月
产品物理化学光谱特性
分子量:350.25
溶剂:水
Ex(nm):358
Em(nm):461
产品介绍
DAPI是一种与DNA结合强烈的荧光染色剂。它广泛用于荧光显微镜。由于DAPI通过完整的细胞膜,它可用于除固定细胞外的活细胞染色。对于荧光显微镜,DAPI被紫外线激发。当与双链DNA结合时,其最大吸收在358nm,其发射最大值在461nm。DAPI的一个缺点是其排放相当广泛。DAPI也与RNA结合,但是它不像它与DNA结合时那样强烈地发荧光。 当与RNA结合时,其发射偏移至约500nm .DAPI的蓝色发射便于多重测定,因为DAPI与荧光素和绿色荧光蛋白(GFP)等绿色荧光分子或红色荧光染料之间的荧光重叠非常少。除了标记细胞核外,DAPI还用于检测细胞培养物中的支原体或病毒DNA。
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参考文献
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相关产品
产品名称 | 货号 |
DAPI(4′,6-二脒基-2-苯基吲哚二盐酸盐)CAS 28718-90-3 | Cat#17510 |