锌离子荧光探针TSQ CAS 109628-27-5 货号21254-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

锌离子荧光探针TSQ CAS 109628-27-5

锌离子荧光探针TSQ CAS 109628-27-5

锌离子荧光探针TSQ CAS 109628-27-5    货号21254 货号 21254 存储条件 在零下15度以下保存, 避免光照
规格 5 mg 价格 1008
Ex (nm) 344 Em (nm) 385
分子量 328.38 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:21254

产品名称:锌离子荧光探针TSQ

CAS:109628-27-5

规格:5mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:328.38

溶剂:DMSO

激发波长(nm):344

发射波长(nm):385

 

产品介绍

锌离子荧光探针TSQ是美国AAT Bioquest生产的用于检测锌离子的荧光探针。TSQ与Zn离子结合后,吸收波长和发射波长分别位于334nm和495nm。在495nm处可以发出很强的荧光。作为一个中性pH值的荧光探针,它已经被证明是一个具有很好的选择性、无毒的荧光探针。TSQ的水溶性不是很好,这使它不适合在活组织中对锌进行测定和成像。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的锌离子荧光探针TSQ。 

 

参考文献

The role of nitric oxide synthase in an early phase Cd-induced acute cytotoxicity in MCF-7 cells
Authors: Lingying Zhong, Lumei Wang, Lurong Xu, Qunlu Liu, Linlei Jiang, Yuee Zhi, Wei Lu, Pei Zhou
Journal: Biological trace element research (2015): 130–138

The role of NOS-mediated ROS accumulation in an early phase Cu-induced acute cytotoxicity in MCF-7 cells
Authors: Lingying Zhong, Lumei Wang, Lurong Xu, Qunlu Liu, Linlei Jiang, Yuee Zhi, Wei Lu, Pei Zhou
Journal: Biometals (2015): 113–122

说明书
锌离子荧光探针TSQ CAS 109628-27-5.pdf

吲哚菁绿ICG炔烃 CAS 1622335-40-3 货号986-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

吲哚菁绿ICG炔烃 CAS 1622335-40-3

吲哚菁绿ICG炔烃 CAS 1622335-40-3

吲哚菁绿ICG炔烃 CAS 1622335-40-3    货号986 货号 986 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 2604
Ex (nm) 789 Em (nm) 814
分子量 768.03 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:986

产品名称:吲哚菁绿ICG炔烃

CAS:1622335-40-3

规格:1mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:768.03

外观:固体

溶剂:DMSO

激发波长(nm):780

发射波长(nm):800

 

产品介绍

吲哚菁绿(ICG)是一种三碳菁型染料,具有NIR吸收特性(峰值吸收约780 nm),最大发射波长约为800 nm。这种染料也称为Cardio Green和其他一些较不常见的商品名。 FDA批准了非侵入性近红外(NIR)荧光成像染料ICG进行眼科血管造影,以确定心输出量以及肝血流量和功能。由于红外频率穿透视网膜层,因此与荧光素血管造影相比,ICG血管造影可以成像更深的循环模式。 ICG与血浆蛋白紧密结合,并局限于血管系统。 ICG的半衰期为150到180秒,只能从肝脏转移至胆汁而从循环系统中移除。最近的一项研究表明,ICG可在注射后20分钟内靶向动脉粥样硬化,并为体内检测动脉粥样硬化兔中富含脂质,发炎,冠状动脉粥样斑块提供了足够的信号增强。离体荧光反射成像显示,与注射盐水的带有粥样斑块的兔子相比,注射了ICG的带有粥样斑块的兔子的斑块靶标与背景比率高。它还可用于其他医学诊断和癌症患者中,用于实体瘤的检测,淋巴结的定位以及在重建手术期间的血管造影,视网膜和脉络膜血管的可视化以及光动力疗法。在癌症诊断和治疗中,ICG既可以用作显像染料又可以用作热疗剂。在可见光范围内几乎没有吸收,这是由于低自发荧光,组织吸收和在近红外波长(700-900 nm)下的散射引起的。这种ICG炔烃可用于通过众所周知的点击化学方法化学选择性标记叠氮基(叠氮化物)标记的生物分子(如蛋白质,脂质,核酸,糖)。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的吲哚菁绿ICG炔烃。 

点击查看光谱

 

参考文献

Assessment of Lexiscan for Blood Brain Barrier disruption to facilitate Fluorescence brain imaging
Authors: Pak, Rebecca W and Le, Hanh and Valentine, Heather and Thorek, Daniel and Rahmim, Arman and Wong, Dean and Kang, Jin U
Journal: (2017): ATu3B–2

Bioengineering of injectable encapsulated aggregates of pluripotent stem cells for therapy of myocardial infarction
Authors: Zhao, Shuting and Xu, Zhaobin and Wang, Hai and Reese, Benjamin E and Gushchina, Liubov V and Jiang, Meng and Agarwal, Pranay and Xu, Jiangsheng and Zhang, Mingjun and Shen, Rulong and others
Journal: Nature Communications (2016): 13306

Deep Photoacoustic/Luminescence/Magnetic Resonance Multimodal Imaging in Living Subjects Using High-Efficiency Upconversion Nanocomposites
Authors: Liu, Yu and Kang, Ning and Lv, Jing and Zhou, Zijian and Zhao, Qingliang and Ma, Lingceng and Chen, Zhong and Ren, Lei and Nie, Liming
Journal: Advanced Materials (2016)

Single-Layer MoS2 Nanosheets with Amplified Photoacoustic Effect for Highly Sensitive Photoacoustic Imaging of Orthotopic Brain Tumors
Authors: Chen, Jingqin and Liu, Chengbo and Hu, Dehong and Wang, Feng and Wu, Haiwei and Gong, Xiaojing and Liu, Xin and Song, Liang and Sheng, Zonghai and Zheng, Hairong
Journal: Advanced Functional Materials (2016)

A simple and effective technique for identification of intersegmental planes by infrared thoracoscopy after transbronchial injection of indocyanine green
Authors: Sekine Y, Ko E, Oishi H, Miwa M.
Journal: J Thorac Cardiovasc Surg. (2012)

Adverse Reaction in Patients with Drug Allergy History After Simultaneous Intravenous Fundus Fluorescein Angiography and Indocyanine Green Angiography
Authors: Su Z, Ye P, Teng Y, Zhang L, Shu X.
Journal: J Ocul Pharmacol Ther. (2012)

An innovative method for detecting surgical errors using indocyanine green angiography during carotid endarterectomy: a preliminary investigation
Authors: Lee CH, Jung YS, Yang HJ, Son YJ, Lee SH.
Journal: Acta Neurochir (Wien) (2012): 67

Anatomic response of occult choroidal neovascularization to intravitreal ranibizumab: a study by indocyanine green angiography
Authors: Querques G, Tran TH, Forte R, Querques L, B and ello F, Souied EH.
Journal: Graefes Arch Clin Exp Ophthalmol (2012): 479

Applicability of radiocolloids, blue dyes and fluorescent indocyanine green to sentinel node biopsy in melanoma
Authors: Uhara H, Yamazaki N, Takata M, Inoue Y, Sakakibara A, Nakamura Y, Suehiro K, Yamamoto A, Kamo R, Mochida K, Takenaka H, Yamashita T, Takenouchi T, Yoshikawa S, Takahashi A, Uehara J, Kawai M, Iwata H, Kadono T, Kai Y, Watanabe S, Murata S, Ikeda T, Fukamizu H, Tanaka T, Hatta N, Saida T.
Journal: J Dermatol (2012): 336

Application of indocyanine green videoangiography in surgery for spinal vascular malformations
Authors: Misra BK, Pur and are HR.
Journal: J Clin Neurosci. (2012)

说明书
吲哚菁绿ICG炔烃 CAS 1622335-40-3.pdf

Texas 红-X,酸 CAS 199745-67-0 货号469-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Texas 红-X,酸 CAS 199745-67-0

Texas 红-X,酸 CAS 199745-67-0

Texas 红-X,酸  CAS 199745-67-0    货号469 货号 469 存储条件 在零下15度以下保存, 避免光照
规格 10 mg 价格 2388
Ex (nm) 595 Em (nm) 613
分子量 719.87 溶剂 DMF
产品详细介绍

简要概述

产品基本信息

货号:469

产品名称:Texas 红-X,酸

CAS:199745-67-0

规格:10mg

储存条件:-15℃,避光干燥

保质期:12个月

 

物理化学光谱特性

分子量:719.87

溶剂:DMF

Ex(nm):595

Em(nm):613

 

产品介绍

Texas Red-X,酸是美国AAT Bioquest生产的荧光染料,与TexasRed®相比,Texas Red-X染料在荧光团和羧基之间包含一个额外的七原子氨基己酰基间隔基(“X”)。该间隔物有助于将荧光团与其连接点分开,从而潜在地减少了荧光团与其结合的生物分子之间的相互作用。该产品是单一的5个异构体,可提供更可重复的结果。它具有与德州红(Texas Red)相同的光谱特性,可用于构建最大激发/发射〜595/615 nm的亮红色荧光生物共轭物(TexasRed®是ThermoFisher的商标)。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Texas Red-X,酸。 

点击查看光谱

 

参考文献

Blood-brain barrier permeability towards small and large tracers in a mouse model of osmotic demyelination syndrome.
Authors: Scalisi, Joshua and Balau, Benoît and Deneyer, Lynn and Bouchat, Joanna and Gilloteaux, Jacques and Nicaise, Charles
Journal: Neuroscience letters (2021): 135665

Detection of Tumor-Specific PTPmu in Gynecological Cancer and Patient Derived Xenografts.
Authors: Vincent, Jason and Craig, Sonya E L and Johansen, Mette L and Narla, Jyosthna and Avril, Stefanie and DiFeo, Analisa and Brady-Kalnay, Susann M
Journal: Diagnostics (Basel, Switzerland) (2021)

Interactions of different lipoproteins with supported phospholipid raft membrane (SPRM) patterns to understand similar in-vivo processes.
Authors: Sapuri-Butti, Annapoorna R and Wang, Limin and Tetali, Sarada D and Rutledge, John C and Parikh, Atul N
Journal: Biochimica et biophysica acta. Biomembranes (2021): 183535

Novel aptasensor-based assay of sonic hedgehog ligand for detection of portal vein invasion of hepatocellular carcinoma.
Authors: Li, Hui-Yan and Yin, Fang-Fei and Li, Xin-Yi and Jia, Wan-Nan and Ding, Jia and Zhang, Li and Wang, Zhong-Hua and Hu, Qin-Qin and Zuo, Jie-Liang and Jia, Hu-Liang and Wang, Li-Hua and Fan, Chun-Hai and Gu, Hong-Zhou and Wu, Jian
Journal: Biosensors & bioelectronics (2021): 112738

Quirks of dye nomenclature. 16. Dyes, and a pigment, named after places.
Authors: Cooksey, C J
Journal: Biotechnic & histochemistry : official publication of the Biological Stain Commission (2021): 1-15

Visualization and quantification of pancreatic tumor stroma in fresh tissue via ultraviolet surface excitation.
Authors: Vincent, Phuong and Bruza, Petr and Palisoul, Scott M and Gunn, Jason R and Samkoe, Kimberley S and Hoopes, P Jack and Hasan, Tayyaba and Pogue, Brian W
Journal: Journal of biomedical optics (2021)

A multifunctional DNA nanostructure based on multicolor FRET for nuclease activity assay.
Authors: Hu, Juan and Li, Wen-Can and Qiu, Jian-Ge and Jiang, BingHua and Zhang, Chun-Yang
Journal: The Analyst (2020): 6054-6060

A new aminoglycoside etimicin shows low nephrotoxicity and ototoxicity in zebrafish embryos.
Authors: Shao, Weihao and Zhong, Dan and Jiang, Haowei and Han, Yujie and Yin, Yu and Li, Ruining and Qian, Xiuping and Chen, Daijie and Jing, Lili
Journal: Journal of applied toxicology : JAT (2020)

Acoustic Poration and Dynamic Healing of Mammalian Cell Membranes during Inkjet Printing.
Authors: Barui, Srimanta and Saunders, Rachel E and Naskar, Sharmistha and Basu, Bikramjit and Derby, Brian
Journal: ACS biomaterials science & engineering (2020): 749-757

Characterization of the Blood-Brain Barrier Integrity and the Brain Transport of SN-38 in an Orthotopic Xenograft Rat Model of Diffuse Intrinsic Pontine Glioma.
Authors: Chaves, Catarina and Declèves, Xavier and Taghi, Meryam and Menet, Marie-Claude and Lacombe, Joelle and Varlet, Pascale and Olaciregui, Nagore G and Carcaboso, Angel M and Cisternino, Salvatore
Journal: Pharmaceutics (2020)

 

相关产品 

产品名称 货号
Texas Red-X,琥珀酰亚胺酯 CAS 216972-99-5 Cat#474

说明书
Texas 红-X,酸 CAS 199745-67-0.pdf

6-DTAF CAS 118357-32-7 货号205-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

6-DTAF CAS 118357-32-7

6-DTAF CAS 118357-32-7

6-DTAF CAS 118357-32-7    货号205 货号 205 存储条件 在零下15度以下保存, 避免光照
规格 1 g 价格 25836
Ex (nm) 498 Em (nm) 517
分子量 495.28 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:205

产品名称:6-DTAF 

CAS:118357-32-7

规格:1g

储存条件:-15℃,避光干燥

保质期:12个月

 

物理化学光谱特性

分子量:495.28

溶剂:DMSO

Ex(nm):497

Em(nm):516

 

产品介绍

6-DTAF是美国AAT Bioquest生产的荧光染料,与5-DTAF相比,6-异构体可更好地用于寡核苷酸及核酸的标记。如同5-DTAF,6-DTAF不仅可与氨基发生反应,还能与巯基反应甚至可与羟基反应,如多糖及水溶液中pH值大于9的其它醇类直接反应。由于其高活性,6-DTAF也可用于标记碳水化合物。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的6-DTAF。 

点击查看光谱

 

参考文献

Fluorescently labeled collagen binding proteins allow specific visualization of collagen in tissues and live cell culture
Authors: Krahn KN, Bouten CV, van Tuijl S, van Zandvoort MA, Merkx M.
Journal: Anal Biochem (2006): 177

Mitomycin C alters corneal stromal wound healing and corneal haze in rabbits after argon-fluoride excimer laser photorefractive keratectomy
Authors: Lai YH, Wang HZ, Lin CP, Chang SJ.
Journal: J Ocul Pharmacol Ther (2004): 129

Fluorescent cellulose microfibrils as substrate for the detection of cellulase activity
Authors: Helbert W, Chanzy H, Husum TL, Schulein M, Ernst S.
Journal: Biomacromolecules (2003): 481

Microbial activity in soils following steam treatment
Authors: Richardson RE, James CA, Bhupathiraju VK, Alvarez-Cohen L.
Journal: Biodegradation (2002): 285

Effect of collagenase inhibitors on corneal haze after PRK
Authors: Corbett MC, O’Brart DP, Patmore AL, Marshall J.
Journal: Exp Eye Res (2001): 253

Glutamic acid decarboxylase-like immunoreactive axon terminals in synaptic contact with mesencephalic trigeminal nucleus neurons in the rat
Authors: Chen P, Li J, Mizuno N.
Journal: Neurosci Lett (2001): 167

Redistribution of plasma-membrane surface molecules during formation of the Leishmania amazonensis-containing parasitophorous vacuole
Authors: Henriques C, de Souza W.
Journal: Parasitol Res (2000): 215

Application of a tetrazolium dye as an indicator of viability in anaerobic bacteria
Authors: Bhupathiraju VK, Hernandez M, Landfear D, Alvarez-Cohen L.
Journal: J Microbiol Methods (1999): 231

Leakage responses to L-NAME differ with the fluorescent dye used to label albumin
Authors: Rumbaut RE, Harris NR, Sial AJ, Huxley VH, Granger DN.
Journal: Am J Physiol (1999): H333

Collagen fiber sliding during ligament growth and contracture
Authors: Wood ML, Lester GE, Dahners LE.
Journal: J Orthop Res (1998): 438

说明书
6-DTAF CAS 118357-32-7.pdf

D-荧光素 游离酸 CAS 2591-17-5 货号12503-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

D-荧光素 游离酸 CAS 2591-17-5

D-荧光素 游离酸 CAS 2591-17-5

D-荧光素 游离酸 CAS 2591-17-5    货号12503 货号 12503 存储条件 在零下15度以下保存, 避免光照
规格 1 g 价格 7296
Ex (nm) 328 Em (nm) 533
分子量 280.32 溶剂 DMSO
产品详细介绍

简要概述

D-荧光素是最流行的多功能生物发光底物。萤火虫荧光素酶/荧光素生物发光系统存在于萤火虫(Photinus pyralis)和其他几种甲虫中。荧光素酶通过二氧杂环丁酮中间体氧化ATP进行活化荧光素。萤火虫荧光素酶通过荧光素的ATP依赖性氧化产生光。来自该反应的560nm化学发光在数秒内达到峰值,当荧光素和ATP过量存在时,光输出与荧光素酶活性成比例。萤火虫荧光素酶长期以来与抗体结合,并在荧光素作为检测底物的免疫测定中用作标记物。与HRP和碱性磷酸酶相比,荧光素酶对化学修饰的耐受性较差。该酶的一个特别优点是除了其高灵敏度之外,在哺乳动物组织中存在低内源荧光素酶活性。荧光素酶的另一个重要用途是卫生监测领域,荧光素酶/荧光素系统可用于检测污染,因为存在于所有生物体中的ATP需要发光,这种ATP生物发光的主要应用是通过测试食品加工厂中的表面来确定质量,以确定是否存在设备或产品的污染。D-荧光素 游离酸 D-Luciferin 是美国AAT Bioquest 的产品。

D-荧光素钾盐及D-荧光素使用中的常见问题

金畔问号:荧光素酶到底该如何检测?

产品说明书

操作方法 

以下方案是钾盐和钠盐制备的一个例子,它可以适用于大多数细胞类型和体内动物用途。

1.用于体外生物发光图像测定的实施方案

1.1在无菌水中制备100mM(100-200X)荧光素原液,混合均匀。随配随用,或使用等分试样,其他等分储存在-20°C,避免冻融循环,避免暴露在光线下。

1.2在预热的组织培养基中制备0.5-1mM D-荧光素的工作溶液。

1.3从培养的细胞中分离培养基。

1.4将荧光素工作溶液加入细胞中,并在成像前将细胞在37°C孵育5-10分钟。

 

2.用于体内生物发光图像测定的实施方案

2.1在DPBS中制备15mg / mL荧光素储备溶液,不含Mg2+和Ca2+。 混合均匀。

2.2通过0.2μm过滤器过滤灭菌溶液。随配随用或单独使用等分试样,其余等分储存在-20°C,避免冻融循环,避免暴露在光线下。

2.3在动物体重150mg / kg(或10μL/ g荧光素储备溶液)成像前10-15分钟在腹膜内(i.p.)注射荧光素。

注意:应对每种动物模型进行荧光素的动力学研究,以确定峰值信号时间。

 

3.荧光素报告分析分子测定的实施方案

3.1在无菌水中制备100mM荧光素储备溶液。 随配随用或单独使用等分试样,其余等分储存在-20°C,避免冻融循环,避免暴露在光线下。

3.2制备1mM D-荧光素的工作溶液,其中含有3mM ATP,1mM DTT和15mM MgSO4的25mM tricine缓冲液,pH7.8。

3.3将5-10μl细胞裂解液移入微孔板中。使用不含裂解液的裂解试剂或缓冲液作为空白对照。

3.4根据制造商的说明,使用荧光素工作溶液的普利光度计。

3.5注入200μl荧光素工作溶液。

 

注意1:D-荧光素钾盐溶于无菌水和缓冲液,溶解度可高达25mg/mL。一般使用浓度为3-15 mg/mL。溶液的pH值、溶液中的氧气和保存时间对其保存过程中的稳定性非常重要。当溶液的pH<6.5(发生水解作用)或>7.5(发生消旋化作用,D型转化为L型)的情况下,D-荧光素钾盐相对不稳定。如果溶液中存在少量的氧气,将加速D-荧光素钾的降解速度。储备溶液可以在不含ATP的水中制备,并在-20°C下避光储存。必须用适当的碱中和游离酸溶解。

注意2:D-荧光素可与任何现有的文献或ATP分析系统一起使用。

注意3:如果检测ATP,请戴上手套并使用无ATP容器,尽量减少所有可能的ATP污染源。仅使用无菌无ATP水和试剂。使用高压灭菌水进行所有试剂制备。

 

参考文献

C3-Luc Cells Are an Excellent Model for Evaluation of Cellular Immunity following HPV16L1 Vaccination
Authors: Li-Li Li, He-Rong Wang, Zhi-Yi Zhou, Jing Luo, Xiao-Li Wang, Xiang-Qian Xiao, Yu-Bai Zhou, Yi Zeng
Journal: PloS one (2016): e0149748

Genome-wide microRNA analysis identifies miR-188-3p as novel prognostic marker and molecular factor involved in colorectal carcinogenesis
Authors: Martin Pichler, Verena Stiegelbauer, Petra Vychytilova-Faltejskova, Cristina Ivan, Hui Ling, Elke Winter, Xinna Zhang, Matthew Goblirsch, Annika Wulf-Goldenberg, Masahisa Ohtsuka
Journal: American Association for Cancer Research (2016): clincanres–0497

Identification of a Novel Protein Kinase A Inhibitor by Bioluminescence-Based Screening
Authors: Tetsuya Ishimoto, Kenji Azechi, Hisashi Mori
Journal: Biological and Pharmaceutical Bulletin (2015): 1969–1974

Discovery of novel adenylyl cyclase inhibitor by cell-based screening
Authors: Hiroki Mano, Tetsuya Ishimoto, Takuya Okada, Naoki Toyooka, Hisashi Mori
Journal: Biological and Pharmaceutical Bulletin (2014): 1689–1693

说明书
D-荧光素 游离酸 CAS 2591-17-5.pdf