Buccutite FOL,马来酰亚胺 [FOLM]
|
货号 |
5356 |
存储条件 |
在零下15度以下保存, 避免光照 |
规格 |
2 umoles |
价格 |
2388 |
Ex (nm) |
|
Em (nm) |
|
分子量 |
523.63 |
溶剂 |
DMSO |
产品详细介绍 |
简要概述
产品基本信息
货号:5356
产品名称:Buccutite FOL,马来酰亚胺 [FOLM]
规格:2 umoles
储存条件:-15℃避光防潮
保质期:12个月
产品物理化学光谱特性
外观:固体
分子量:523.63
溶剂:DMSO
产品介绍
Buccutite 交联技术提供了最方便、最有效的交联方法,以高共轭产率连接两个生物分子。该方法使用一对交联剂:Buccutite MTA 和 Buccutite FOL。 MTA 被添加到一个分子中,而 FOL 被添加到另一个分子中。通过在中性条件下混合 Molecule-1-Buccutite MTA 和 Molecule-2-Buccutite FOL 来引发交联反应。Buccutite FOL 马来酰亚胺 (FOLM) 的使用方式与广泛用于交联蛋白质的 SMCC 相同。 FOLM 的一端(通过马来酰亚胺)与还原抗体(通过 TCEP 或 DTT)中发现的半胱氨酸硫醇 (-SH) 反应,以产生 FOL 修饰的还原抗体。 Buccutite 交联反应在极其温和和中性的条件下发生,不需要任何催化剂,而 SMCC 交联需要高浓度的蛋白质。此外,SMCC 修饰的蛋白质极不稳定,通常会自我反应,因为蛋白质通常同时含有胺和硫醇基团,这会导致大量的同交联。Buccutite 交联反应在极其温和和中性的条件下发生,无需任何催化剂,它比SMCC更加稳定高效。
参考文献
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说明书
Buccutite FOL,马来酰亚胺 [FOLM].pdf