蛋白酶荧光底物Bis-Arg-rhodamine 110 货号13481-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

蛋白酶荧光底物Bis-Arg-rhodamine 110

蛋白酶荧光底物Bis-Arg-rhodamine 110

蛋白酶荧光底物Bis-Arg-rhodamine 110    货号13481 货号 13481 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 3924
Ex (nm) 500 Em (nm) 522
分子量 1098.81 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:13481

产品名称:Bis-Arg-罗丹明110

规格:1mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:1098.81

外观:固体

溶剂:DMSO

激发波长(nm):498

发射波长(nm):520

 

产品介绍

Bis-Arg-罗丹明110是一种氨基肽酶和内肽酶荧光底物,是美国AAT Bioquest研发生产的罗丹明类染料,用于检测人α-凝血酶的酶活性。非荧光底物会产生亮绿色的荧光罗丹明产物,并且可以被FITC滤波片组轻松检测到。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的罗丹明染料。 

点击查看光谱

说明书
蛋白酶荧光底物Bis-Arg-rhodamine 110.pdf

蛋白酶荧光底物Benzoyl-Arg-AMC 货号13478-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

蛋白酶荧光底物Benzoyl-Arg-AMC

蛋白酶荧光底物Benzoyl-Arg-AMC

蛋白酶荧光底物Benzoyl-Arg-AMC    货号13478 货号 13478 存储条件 在零下15度以下保存, 避免光照
规格 5 mg 价格 1272
Ex (nm) 341 Em (nm) 441
分子量 549.50 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:13478

产品名称:蛋白酶荧光底物Benzoyl-Arg-AMC

规格:5mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:549.50

外观:橙色粉末

溶剂:DMSO

激发波长(nm):351

发射波长(nm):430

 

产品介绍

蛋白酶荧光底物Benzoyl-Arg-AMC是美国AAT Bioquest生产的荧光底物,该苯甲酰-Arg-AMC是一种荧光氨基肽酶和内肽酶底物,用于检测人α-凝血酶的酶活性。 非荧光底物会产生亮蓝色荧光AMC产品,该产品的发射光谱可以通过DAPI滤光片组轻松检测到。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的蛋白酶荧光底物Benzoyl-Arg-AMC。 

点击查看光谱

 

参考文献

A fluorescence lifetime-based assay for protease inhibitor profiling on human kallikrein 7
Authors: Doering K, Meder G, Hinnenberger M, Woelcke J, Mayr LM, Hassiepen U.
Journal: J Biomol Screen (2009): 1

A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease
Authors: Bodenreider C, Beer D, Keller TH, Sonntag S, Wen D, Yap L, Yau YH, Shochat SG, Huang D, Zhou T, Caflisch A, Su XC, Ozawa K, Otting G, Vasudevan SG, Lescar J, Lim SP.
Journal: Anal Biochem (2009): 195

A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates
Authors: Nakata H, Ohtsuki T, Sisido M.
Journal: Anal Biochem (2009): 121

Dual-function probe to detect protease activity for fluorescence measurement and 19F MRI
Authors: Mizukami S, Takikawa R, Sugihara F, Shirakawa M, Kikuchi K.
Journal: Angew Chem Int Ed Engl (2009): 3641

Fluorescence resonance energy transfer-based assay for characterization of hepatitis C virus NS3-4A protease activity in live cells
Authors: Sabariegos R, Picazo F, Domingo B, Franco S, Martinez MA, Llopis J.
Journal: Antimicrob Agents Chemother (2009): 728

Improved detection of ovarian cancer metastases by intraoperative quantitative fluorescence protease imaging in a pre-clinical model
Authors: Sheth RA, Upadhyay R, Stangenberg L, Sheth R, Weissleder R, Mahmood U.
Journal: Gynecol Oncol (2009): 616

Label-free, homogeneous, and fluorescence “turn-on” detection of protease using conjugated polyelectrolytes
Authors: An L, Liu L, Wang S.
Journal: Biomacromolecules (2009): 454

Real-time detection of caspase-3-like protease activation in vivo using fluorescence resonance energy transfer during plant programmed cell death induced by ultraviolet C overexposure
Authors: Zhang L, Xu Q, Xing D, Gao C, Xiong H.
Journal: Plant Physiol (2009): 1773

Real-time monitoring of human enterovirus (HEV)-infected cells and anti-HEV 3C protease potency by fluorescence resonance energy transfer
Authors: Tsai MT, Cheng YH, Liu YN, Liao NC, Lu WW, Kung SH.
Journal: Antimicrob Agents Chemother (2009): 748

[Prokaryotic expression and Identification of dual-fluorescence fusion proteins of small ubiquitin-like modifier and sentrin-specific protease]
Authors: Wang X, Guo J, Wang J, Li S, Sun L, Lu J.
Journal: Sheng Wu Gong Cheng Xue Bao (2009): 701

说明书
蛋白酶荧光底物Benzoyl-Arg-AMC.pdf

肽底物DLDVPIPGRFDRRVpSVAAE(H-Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser(PO₃H₂)-Val-Ala-Ala-Glu-OH) 货号31700-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

肽底物DLDVPIPGRFDRRVpSVAAE(H-Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser(PO₃H₂)-Val-Ala-Ala-Glu-OH)

肽底物DLDVPIPGRFDRRVpSVAAE(H-Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser(PO₃H₂)-Val-Ala-Ala-Glu-OH)

肽底物DLDVPIPGRFDRRVpSVAAE(H-Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser(PO₃H₂)-Val-Ala-Ala-Glu-OH)    货号31700 货号 31700 存储条件 在零下15度以下保存, 避免光照
规格 1 mg 价格 1836
Ex (nm) Em (nm)
分子量 2192.34 溶剂 DMSO
产品详细介绍

简要概述

产品基本信息

货号:31700

产品名称:DLDVPIPGRFDRRVpSVAAE(H-Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser(PO₃H₂)-Val-Ala-Ala-Glu-OH)

规格:1mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:2192.34

溶剂:DMSO

 

产品介绍

DLDVPIPGRFDRRVpSVAAE(H-Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser(PO 3 H 2)-Val-Ala-Ala-Glu-OH)是非常好钙调神经磷酸酶的肽底物(蛋白质磷酸酶2B,PP2B)。该序列衍生自II型PKA调节亚基(RII),并在Ser残基上被磷酸化。钙调神经磷酸酶是一种钙依赖性丝氨酸/苏氨酸磷酸酶,参与多种信号传导途径。钙调磷酸酶在磷酸酶中是不同的,因为其活性需要钙,并且对阻断相关磷酸酶PP1A和PP2A的化合物的抑制作用不敏感。当前的常用技术通过测量释放的放射性磷酸盐或用孔雀石绿检测游离磷酸盐来定量钙调神经磷酸酶的活性。两种方法都涉及技术挑战并且具有不良特征。 AAT Bioquest开发了一些出色的磷酸盐检测法,可用于监测钙调神经磷酸酶对DLDVPIPGRFDRRVpSVAAE脱磷酸作用中磷酸盐的释放。 PhosphoWorks 比色磷酸盐测定试剂盒(21665)和PhosphoWorks 比色MESG磷酸盐测定试剂盒(21659)可用于磷酸盐的比色测量。为了获得更高的灵敏度,可以使用PhosphoWorks 荧光磷酸盐检测试剂盒(21660)对荧光粉的释放进行荧光定量。

 

图示

肽底物DLDVPIPGRFDRRVpSVAAE(H-Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser(PO₃H₂)-Val-Ala-Ala-Glu-OH)    货号31700

图1. DLDVPIPGRFDRRVpSVAAE(H-Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser(PO₃H₂)-Val-Ala-Ala-Glu -OH)是钙调神经磷酸酶(蛋白质磷酸酶2B,PP2B)的极佳肽底物。 AAT Bioquest开发了一些出色的磷酸盐测定法,可用于监测钙调神经磷酸酶对DLDVPIPGRFDRRVpSVAAE脱磷酸作用中磷酸盐的释放。 PhosphoWorks 比色磷酸盐测定试剂盒(21665)和PhosphoWorks 比色MESG磷酸盐测定试剂盒(21659)可用于磷酸盐的比色测量。为了获得更高的灵敏度,可以使用PhosphoWorks 荧光磷酸盐检测试剂盒(21660)对荧光的释放进行荧光定量。

 

参考文献

Genome-wide analysis of the specificity and mechanisms of replication infidelity driven by imbalanced dNTP pools.
Authors: Watt, Danielle L and Buckland, Robert J and Lujan, Scott A and Kunkel, Thomas A and Chabes, Andrei
Journal: Nucleic acids research (2016): 1669-80

Fluorescence polarization-based method with bisulfite conversion-specific one-label extension for quantification of single CpG dinucleotide methylation.
Authors: Li, Shufen and Wang, Zhongju and Zhou, Lin and Luo, Fu and Zhao, Cunyou
Journal: Genome (2015): 357-63

Hi-C in Budding Yeast.
Authors: Belton, Jon-Matthew and Dekker, Job
Journal: Cold Spring Harbor protocols (2015): 649-61

Transcriptome analysis of Capsicum annuum varieties Mandarin and Blackcluster: assembly, annotation and molecular marker discovery.
Authors: Ahn, Yul-Kyun and Tripathi, Swati and Kim, Jeong-Ho and Cho, Young-Il and Lee, Hye-Eun and Kim, Do-Sun and Woo, Jong-Gyu and Cho, Myeong-Cheoul
Journal: Gene (2014): 494-9

Structures of an apo and a binary complex of an evolved archeal B family DNA polymerase capable of synthesising highly cy-dye labelled DNA.
Authors: Wynne, Samantha A and Pinheiro, Vitor B and Holliger, Philipp and Leslie, Andrew G W
Journal: PloS one (2013): e70892

说明书
肽底物DLDVPIPGRFDRRVpSVAAE(H-Asp-Leu-Asp-Val-Pro-Ile-Pro-Gly-Arg-Phe-Asp-Arg-Arg-Val-Ser(PO₃H₂)-Val-Ala-Ala-Glu-OH).pdf