APC/Cy7 抗人 CD43 抗体 *HI161* 货号104311D1-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

APC/Cy7 抗人 CD43 抗体 *HI161*

APC/Cy7 抗人 CD43 抗体 *HI161*

货号 104311D1 存储条件
规格 100 tests 价格 6900
Ex (nm) 754 Em (nm) 779
分子量 N/A 溶剂
产品详细介绍

简要概述

产品基本信息

货号:104311D1

产品名称:APC/Cy7 抗人 CD43 抗体 *HI161*

规格:100 tests

 

产品介绍

HI161 单克隆抗体与人 CD43 反应,CD43 是一种 95 – 135 kD 的跨膜蛋白,通常位于浆细胞、胸腺细胞、中性粒细胞、骨髓瘤和 T 细胞的表面。在一些生物体中,CD43在下调细胞粘附、促进肿瘤坏死因子的生物合成过程和负调节T细胞增殖方面发挥作用。从研究的角度来看,由于它与重要的大分子/配体(如 EZR)相关,因此具有生物学意义。 CD43 是一种相当少见的抗体靶标,在过去十年中发表的文章仅仅 5000 篇。尽管如此,CD43 已广泛用于免疫学研究,在流式细胞仪应用中经常用作区分细胞类型的表型标记。该抗体通过亲和层析纯化并与 APC/Cy7 (ex/em = 754/779 nm) 偶联。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的APC/Cy7 抗人 CD43 抗体 *HI161*。

点击查看光谱

Buccutite APC-Cy7抗体标记试剂盒 标记25ug抗体 货号1351-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Buccutite APC-Cy7抗体标记试剂盒 标记25ug抗体

Buccutite APC-Cy7抗体标记试剂盒 标记25ug抗体

Buccutite APC-Cy7抗体标记试剂盒 标记25ug抗体    货号1351 货号 1351 存储条件 在2-8度冷藏保存, 避免光照
规格 2 Labelings 价格 3924
Ex (nm) 754 Em (nm) 779
分子量 溶剂
产品详细介绍

简要概述

Buccutite APC-Cy7抗体标记试剂盒提供了一种以微观方式标记抗体的最便捷方法。APC-Cy7是流式细胞仪中常用的颜色。其主要吸收峰位于651nm,发射峰位于~780nm。 AAT Bioquest提供这种Buccutite 快速标记试剂盒,以促进APC-Cy7串联结合抗体和其他蛋白质,如链霉抗生物素蛋白和其他二级试剂。 Buccutite APC-Cy7串联共轭试剂盒提供了一种稳定而方便的方法,可将抗体与APC-Cy7串联结合。该试剂盒包括预活化的APC-Cy7串联和反应缓冲液。整个过程仅需要两次简单的混合,无需进一步纯化。缀合的抗体可用于WB,ELISA和IHC应用。该试剂盒足以进行2次标记反应,每次反应最多25μg抗体。任何新抗体试剂的最佳比例必须通过实验确定。我们的试剂盒提供预活化的APC-Cy7串联,以促进APC-Cy7串联结合抗体和其他蛋白质,如链霉抗生物素蛋白和其他二级试剂。我们的预活化APC-Cy7串联已准备好结合,产生比常规繁琐的基于SMCC的缀合化学高得多的产率。此外,我们的预活化APC-Cy7串联通过其蛋白质丰富的氨基与蛋白质缀合,而SMCC化学靶向必须通过抗体还原而再生的硫醇基团。Buccutite APC-Cy7抗体标记试剂盒是美国AAT Bioquest研发的产品。

点击查看光谱

产品说明书

实验方案

操作步骤

1.运行Antibody-Buccutite MTA反应

1.1将抗体溶液直接加入到Buccutite MTA(组分B)的小瓶中,并通过反复移液几次将其充分混合或将小瓶涡旋几秒钟。

1.2将抗体-Buccutite MTA反应混合物在室温下保持30-60分钟。 注意:如果需要,抗体-Buccutite MTA反应混合物可以旋转或摇动更长时间。

 

2.制备抗体-APC-Cy7缀合物

2.1通过向Buccutite FOL活化的APC-Cy7(组分A)的小瓶中加入50μLddH2O制备Buccutite FOL活化的APC-Cy7溶液,通过反复移液几次充分混合或将小瓶涡旋几秒钟。

2.2将整瓶Buccutite FOL活化的APC-Cy7溶液混合到纯化的抗体-Buccutite MTA溶液(来自步骤纯化抗体-Buccutite MTA溶液)中,充分混合并在室温下旋转混合物1小时。

2.3抗体-APC-Cy7结合物现在可以使用了。 注意:立即使用时,抗体-APC-Cy7结合物需要用您选择的缓冲液稀释。 注意:对于长期储存,需要浓缩或冷冻干燥抗体-APC-Cy7结合物溶液。

 

3.抗体-APC-Cy7共轭物的储存

抗体缀合物应在载体抗体(例如0.1%牛血清白蛋白)存在下以> 0.5mg / mL储存。 当在2mM叠氮化钠存在下储存并避光时,抗体-APC-Cy7缀合物溶液可以在4℃下储存两个月而没有显着变化。 对于更长时间的储存,可以将抗体-APC-Cy7缀合物冻干并在≤-20℃下储存。

 

参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

 

相关产品

产品名称 货号
Buccutite APC-Cy7抗体标记试剂盒 标记100ug抗体 Cat#1321
Buccutite PE-Cy5抗体标记试剂盒 标记100ug抗体 Cat#1322
Buccutite PE-Cy5抗体标记试剂盒 标记25ug抗体 Cat#1340

说明书
Buccutite APC-Cy7抗体标记试剂盒 标记25ug抗体.pdf

Buccutite APC-Cy5.5抗体标记试剂盒(标记25ug抗体) 货号1350-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

Buccutite APC-Cy5.5抗体标记试剂盒(标记25ug抗体)

Buccutite APC-Cy5.5抗体标记试剂盒(标记25ug抗体)

Buccutite APC-Cy5.5抗体标记试剂盒(标记25ug抗体)    货号1350 货号 1350 存储条件 在2-8度冷藏保存, 避免光照
规格 2 Labelings 价格 3924
Ex (nm) 656 Em (nm) 700
分子量 溶剂
产品详细介绍

简要概述

Buccutite APC-Cy5.5抗体标记试剂盒提供了一种以微观方式标记抗体的最便捷方法。APC-Cy5.5是流式细胞仪中常用的颜色。其主要吸收峰位于651nm,发射峰位于~700nm。 AAT Bioquest提供这种Buccutite 快速标记试剂盒,以促进APC-Cy5.5与抗体和其他蛋白质(如链霉抗生物素蛋白和其他二级试剂)的串联结合。 Buccutite APC-Cy5.5串联共轭试剂盒提供了一种稳定而方便的方法,可将抗体与APC-Cy5.5串联结合。该试剂盒包括预活化的APC-Cy5.5串联和反应缓冲液。整个过程仅需要两次简单的混合,无需进一步纯化。缀合的抗体可用于WB,ELISA和IHC应用。该试剂盒足以进行2次标记反应,每次反应最多25μg抗体。任何新抗体试剂的最佳比例必须通过实验确定。我们的试剂盒提供预活化的APC-Cy5.5串联,以促进APC-Cy5.5串联结合抗体和其他蛋白质,如链霉抗生物素蛋白和其他二级试剂。我们的预活化APC-Cy5.5串联已准备好结合,产生比常规繁琐的基于SMCC的缀合化学高得多的产率。此外,我们的预活化APC-Cy5.5串联通过其蛋白质丰富的氨基与蛋白质缀合,而SMCC化学靶向必须通过抗体还原再生的硫醇基团。Buccutite APC-Cy5.5抗体标记试剂盒是美国AAT Bioquest研发的产品。

点击查看光谱

产品说明书

实验方案

操作步骤

1.运行Antibody-Buccutite MTA反应

1.1将抗体溶液直接加入到Buccutite MTA(组分B)的小瓶中,并通过反复移液几次将其充分混合或将小瓶涡旋几秒钟。

1.2将抗体-Buccutite MTA反应混合物在室温下保持30-60分钟。 注意:如果需要,抗体-Buccutite MTA反应混合物可以旋转或摇动更长时间。

 

2.制备抗体-APC-Cy5.5缀合物

2.1通过向Buccutite FOL活化的APC-Cy5.5(组分A)的小瓶中加入50μLddH2O制备Buccutite FOL活化的APC-Cy5.5溶液,通过反复移液几次充分混合或将小瓶涡旋几秒钟。

2.2将整瓶Buccutite FOL活化的APC-Cy5.5溶液混合到纯化的抗体-Buccutite MTA溶液(来自步骤纯化抗体-Buccutite MTA溶液)中,充分混合并在室温下旋转混合物1小时。

2.3抗体-APC-Cy5.5结合物现在可以使用了。 注意:立即使用时,抗体-APC-Cy5.5结合物需要用您选择的缓冲液稀释。 注意:对于长期储存,需要浓缩或冷冻干燥抗体-APC-Cy5.5结合物溶液。

 

3.抗体-APC-Cy5.5共轭物的储存

抗体缀合物应在载体抗体(例如0.1%牛血清白蛋白)存在下以> 0.5mg / mL储存。 当在2mM叠氮化钠存在下储存并避光时,抗体-APC-Cy5.5缀合物溶液可以在4℃下储存两个月而没有显着变化。 对于更长时间的储存,可以将抗体-APC-Cy5.5缀合物冻干并在≤-20℃下储存。

 

参考文献

Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573

Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016

Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598

Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59

Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113

Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749

Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226

[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82

 

相关产品

产品名称  货号
Buccutite APC-Cy5.5抗体标记试剂盒 标记100ug抗体 Cat#1320
Buccutite APC-iFluor 700抗体标记试剂盒 标记100ug抗体 Cat#1319
Buccutite APC-iFluor 700抗体标记试剂盒 标记25ug抗体 Cat#1347

说明书
Buccutite APC-Cy5.5抗体标记试剂盒(标记25ug抗体).pdf

APC-iFluor 750 串联染料 货号2626-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

APC-iFluor 750 串联染料

APC-iFluor 750 串联染料

货号 2626 存储条件 在2-8度冷藏保存, 避免光照
规格 1 mg 价格 2388
Ex (nm) 754 Em (nm) 776
分子量 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:2626

产品名称:APC-iFluor 750 串联染料

规格:1mg

储存条件:2-8℃避光防潮

保质期:6个月

 

产品物理化学光谱特性

外观:固体

溶剂:水

 

产品介绍

串联染料是一类独特的荧光分子,由两种不同的共价连接荧光团、供体(例如 PE 或 APC)和发射较长波长的荧光受体(例如 Texas Red、Cy5、Cy7、iFluor 594 或 iFluor 750组成)。APC-iFluor 750 串联染料是常用 APC-Cy7 的绝佳替代品,具有更高的 FRET 效率和信号。其主要吸收峰位于 651 nm,发射峰位于 ~780 nm。 金畔还提供其预活化的 APC-iFluor 750 串联染料,以促进 APC-iFluor 750 串联与抗体和其他蛋白质(如链霉亲和素和其他二级试剂)的串联。我们的预活化 APC-iFluor 750 可随时进行缀合,其产量比传统繁琐的SMCC 的化学方法高得多。此外,我们的预活化 APC-iFluor 750 串联染料通过蛋白质中丰富的氨基与蛋白质缀合,而 SMCC 化学靶向必须通过抗体还原再生的硫醇基团。

 

参考文献

CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C virus-coinfected patients.
Authors: Laufer, Natalia and Ojeda, Diego and Polo, María Laura and Martinez, Ana and Pérez, Héctor and Turk, Gabriela and Cahn, Pedro and Zwirner, Norberto Walter and Quarleri, Jorge
Journal: World journal of hepatology (2017): 1073-1080

Quantification of mitochondrial reactive oxygen species in living cells by using multi-laser polychromatic flow cytometry.
Authors: De Biasi, Sara and Gibellini, Lara and Bianchini, Elena and Nasi, Milena and Pinti, Marcello and Salvioli, Stefano and Cossarizza, Andrea
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2016): 1106-1110

Analysis of Populations of Memory T-Helper Cells Expressing CXCR3 and CCR6 Chemokine Receptors in Peripheral Blood of Patients with Chronic Viral Hepatitis C.
Authors: Elezov, D S and Kudryavtsev, I V and Arsent’ev, N A and Basin, V V and Esaulenko, E V and Semenov, A V and Totolyan, A A
Journal: Bulletin of experimental biology and medicine (2015): 238-42

Presence of CD34(+)CD38(-)CD58(-) leukemia-propagating cells at diagnosis identifies patients at high risk of relapse with Ph chromosome-positive ALL after allo-hematopoietic SCT.
Authors: Kong, Y and Xu, L-P and Liu, Y-R and Qin, Y-Z and Sun, Y-Q and Wang, Y and Jiang, H and Jiang, Q and Chen, H and Chang, Y-J and Huang, X-J
Journal: Bone marrow transplantation (2015): 348-53

A flow cytometric method for the analysis of macrophages in the vascular wall.
Authors: Moore, Jeffrey P and Sakkal, Samy and Bullen, Michelle L and Kemp-Harper, Barbara K and Ricardo, Sharon D and Sobey, Christopher G and Drummond, Grant R
Journal: Journal of immunological methods (2013): 33-43

Combined normal donor and CLL: Single tube ZAP-70 analysis.
Authors: Degheidy, Heba A and Venzon, David J and Farooqui, Mohammed Z H and Abbasi, Fatima and Arthur, Diane C and Wilson, Wyndham H and Wiestner, Adrian and Stetler-Stevenson, M A and Marti, Gerald E
Journal: Cytometry. Part B, Clinical cytometry (2012): 67-77

The role of CD19 and CD27 in the diagnosis of multiple myeloma by flow cytometry: a new statistical model.
Authors: Cannizzo, Elisa and Carulli, Giovanni and Del Vecchio, Luigi and Ottaviano, Virginia and Bellio, Emanuele and Zenari, Ezio and Azzarà, Antonio and Petrini, Mario and Preffer, Frederic
Journal: American journal of clinical pathology (2012): 377-86

Measurement conditions for flow cytometry analyses of cell lines from urological carcinomas.
Authors: Tölle, Angelika and Abdallah, Ziyad and Jung, Klaus and Bäumler, Hans
Journal: Journal of fluorescence (2010): 779-86

An optimized flow cytometry protocol for analysis of angiogenic monocytes and endothelial progenitor cells in peripheral blood.
Authors: Hristov, Mihail and Schmitz, Susanne and Schuhmann, Christoph and Leyendecker, Thorsten and von Hundelshausen, Philipp and Krötz, Florian and Sohn, Hae-Young and Nauwelaers, Frans A and Weber, Christian
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 848-53

Flow cytometry APC-tandem dyes are degraded through a cell-dependent mechanism.
Authors: Le Roy, Christine and Varin-Blank, Nadine and Ajchenbaum-Cymbalista, Florence and Letestu, Rémi
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2009): 882-90

说明书
APC-iFluor 750 串联染料.pdf

APC-iFluor A7 串联染料 货号2632-AAT Bioquest荧光染料

上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。

APC-iFluor A7 串联染料

APC-iFluor A7 串联染料

货号 2632 存储条件 在2-8度冷藏保存, 避免光照
规格 1 mg 价格 2940
Ex (nm) Em (nm)
分子量 N/A 溶剂 Water
产品详细介绍

简要概述

产品基本信息

货号:2632

产品名称:APC-iFluor A7 串联染料

规格:1mg

储存条件:2-8℃避光防潮

保质期:6个月

 

产品物理化学光谱特性

分子量:N/A

外观:固体

溶剂:水

 

产品介绍

APC-iFluor A7 串联染料是美国AAT Bioquest生产的荧光染料,串联染料是一类独特的荧光分子,由两种不同的共价连接的荧光团、一个供体(例如 PE 或 APC)和一个发射较长波长的荧光受体(例如 Texas Red、Cy5、Cy7、iFluor™ 594 或 iFluor™ 750 )。 APC-Cy7 是用于多色应用的最流行的串联颜色之一。也有一些改进的替代品,例如 APC-Alexa Fluor 750、APC-H7 和 APC-Fire 750。但是,APC-Cy7、APC-Alexa Fluor 750 和 APC-Fire 750 的性能非常相似,并且声称的改进是微不足道的。我们的 APC-A7 比 APC-Cy7、APC-Alexa Fluor 750、APC-H7 和 APC-Fire 750 亮得多。它是对现有 APC-Cy7 串联的真正升级产品。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的APC-iFluor A7 串联染料。 

 

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说明书
APC-iFluor A7 串联染料.pdf