AF488 NHS 酯超纯级 *AF488 与 Alexa Fluor 488 结构相同*

简要概述

产品基本信息

货号：71901

产品名称：AF488 NHS 酯类超纯级 *AF488 与 Alexa Fluor 488 结构相同*

规格：5mg

储存条件：保存在冰箱-15℃干燥

保质期：12个月

产品物理化学光谱特性

分子量：643.40

外观：红色固体

溶剂：DMSO

激发波长(nm)：499

发射波长(nm)：520

产品介绍

AF488 由 AAT Bioquest 制造，具有与 Alexa Fluor® 488 相同的化学结构（Alexa Fluor® 是 ThermoFisher 的商标）。 AF488 NHS 酯（琥珀酰亚胺酯）与 Alexa Fluor® 488 NHS 酯（来自 ThermoFisher 的#A20000 和 A20100）具有相同的分子。它是一种亮绿色荧光染料，最适合与 488 nm 氩激光一起使用。 AF488 染料是水溶性的，从 pH 4 到 pH 10 对 pH 不敏感。AF488 NHS 酯（或琥珀酰亚胺酯）是将这种染料与蛋白质或抗体结合的最方便的胺反应形式。尽管 Alexa Fluor® 488 NHS Ester 是最常见的绿色荧光标记染料之一，但其纯度一直颇具挑战性。 ThermoFisher 提供的纯度约为 50-60%，而其他一些供应商提供的纯度甚至更低。在所有杂质中，我们注意到 AF488 的 Bis-NHS 酯是非常有害的，因为它会产生非荧光产物。这种高纯度 AF488 NHS 酯不含有害的 AF488 Bis-NHS 酯杂质，最低纯度为 >75%。

参考文献

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Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
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Journal: Methods Mol Biol (2017): 21-Sep

Alteration of AMPA Receptor-Mediated Synaptic Transmission by Alexa Fluor 488 and 594 in Cerebellar Stellate Cells
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Journal: eNeuro (2016)

Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments
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Journal: Bioconjug Chem (2015): 593-601

A mouse monoclonal antibody against Alexa Fluor 647
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Journal: Monoclon Antib Immunodiagn Immunother (2014): 109-20

In vivo visualization of GL261-luc2 mouse glioma cells by use of Alexa Fluor-labeled TRP-2 antibodies
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