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钙红素 AM
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货号 | 22011 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 1 mg | 价格 | 2604 | |
| Ex (nm) | 643 | Em (nm) | 663 | |
| 分子量 | ~1000 | 溶剂 | DMSO | |
| 产品详细介绍 | ||||
简要概述
产品基本信息
货号：22011
产品名称：钙红素 AM
规格：1mg
储存条件：-15℃避光防潮
保质期：12个月
产品物理化学光谱特性
分子量：~1000
溶剂：DMSO
激发波长(nm)：643
发射波长(nm)：663
适用仪器
| 流式细胞仪 | |
| 激发： | 633/640nm激光 |
| 发射： | 660/20nm滤波片 |
| 通道： | APC通道 |
| 荧光显微镜 | |
| 激发： | Cy5滤波片 |
| 发射： | Cy5滤波片 |
| 推荐孔板： | 黑色透明 |
产品介绍
钙黄绿素AM是最流行的荧光探针之一，用于标记和监测活细胞的细胞功能。但是，钙黄绿素AM的单色使得无法在多色应用中使用这种有价值的试剂。例如，与GFP相同的颜色，不可能将Calcein AM与GFP转染的细胞结合使用。为了解决钙黄绿素AM的颜色限制，我们开发了Calcein Orange，Calcein Red和Calcein Deep Red。这些新的钙黄绿素AM酯与钙黄绿素AM结合使用可对活细胞进行多色标记和功能分析。非荧光钙红绿素 AM酯很容易进入活细胞并水解，产生强荧光的钙红绿素 （Cat＃：21902）染料。钙红绿素染料可以用通用的Cy5滤波器组进行检测。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的钙红素 AM。
点击查看光谱
图示
图1.用钙黄绿素Deep Red AM酯（Cat＃22011）染色的Jurkat细胞的流式细胞术分析。将Jurkat细胞用HH缓冲液洗涤一次，并在37°C下用含0.02％PF-127（Cat＃20053）和1mM PBC（Cat＃20061）的2 uM Calcein Deep Red AM酯（Cat＃22011）染色30分钟。然后用HH缓冲液洗涤细胞，并重悬于HH缓冲液中。使用蓝色激光APC发射通道，用NovoCyte 3000流式细胞仪测量活细胞（红色）和死细胞（在55°C水浴中处理30分钟，绿色）的荧光强度。
参考文献
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Authors: Zhang, C., Zhang, H., Yu, Y., Wu, S., Chen, F.
Journal: Talanta (2019): 451-456
Calcein Release from Cells In Vitro via Reversible and Irreversible Electroporation
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Journal: J Membr Biol (2018): 119-130
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Journal: Anal Biochem (2018): 19-23
Light scattering corrections to linear dichroism spectroscopy for liposomes in shear flow using calcein fluorescence and modified Rayleigh-Gans-Debye-Mie scattering
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