Cell Meter BX590固定化细胞活性染料
 |
货号 |
22514 |
存储条件 |
在零下15度以下保存, 避免光照 |
| 规格 |
200 Tests |
价格 |
2472 |
| Ex (nm) |
492 |
Em (nm) |
579 |
| 分子量 |
|
溶剂 |
DMSO |
| 产品详细介绍 |
简要概述
产品基本信息
货号：22514
产品名称：Cell Meter BX590固定化细胞活性染料
规格：200 Tests
储存条件：保存在冰箱-15℃干燥
保质期：12个月
产品物理化学光谱特性
分子量：N/A
激发波长(nm)：492
发射波长(nm)：579
适用仪器
| 流式细胞仪 |
|
| 激发： |
480nm激光 |
| 发射： |
575/26nm滤波片 |
| 通道： |
PE通道 |
| 荧光显微镜 |
|
| 激发： |
Cy3/TRITC滤波片组 |
| 发射： |
Cy3/TRITC滤波片组 |
| 推荐孔板： |
黑色透明 |
产品介绍
从活细胞中区分和排除死细胞可以更清晰地分离和鉴定细胞群。Cell Meter BX590固定化细胞活性染料是一类细胞不可渗透的荧光活性染料，它们通过优化以匹配常见流式细胞仪的主要激发激光，例如350、405、488、633和647 nm。这些染料不渗入活细胞，但渗入膜受损的细胞。它们与含胺和硫醇的蛋白质以及其他细胞成分发生不可逆的反应。由于具有受损膜的死细胞或固定细胞更容易与Cell Meter BX590固定化细胞活性染料发生反应，因此比具有完整膜的活细胞的染色更亮，因此这些染料可用于评估哺乳动物细胞的活与死状态。使用这些染料时，需要考虑一些因素，例如，每种染料的滴定，以确保活细胞几乎没有染色。Cell Meter BX590固定化细胞活性染料通过优化，可在488 nm的蓝色激光激发并在590 nm发射。与其他商业上类似的活性染料相比，这种Cell Meter BX590固定化细胞活性染料更加耐用、稳定。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Cell Meter BX590固定化细胞活性染料。
点击查看光谱
图示
图1.通过Cell Meter BX590固定化细胞活性染料检测Jurkat细胞活性。处理Jurkat细胞并用Cell Meter BX590（Cat＃22514）染色，然后固定在3.7％甲醛中，并通过流式细胞仪进行分析。带有PE-TexasRed通道的死细胞数量（蓝峰）很容易与活细胞数量（红峰）区分开，在固定之前和之后获得的结果几乎相同。
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参考文献
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Journal: Methods in enzymology (2020): 239-255
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Journal: Methods in molecular biology (Clifton, N.J.) (2018): 59-67
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Journal: PloS one (2017): e0179768
Analysis of CFSE time-series data using division-, age- and label-structured population models.
Authors: Hross, Sabrina and Hasenauer, Jan
Journal: Bioinformatics (Oxford, England) (2016): 2321-9
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Journal: Human immunology (2016): 1215-1222
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Journal: Allergy (2015): 1222-9
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Journal: Cytometry. Part B, Clinical cytometry (2015): 30-9
Mathematical models for CFSE labelled lymphocyte dynamics: asymmetry and time-lag in division.
Authors: Luzyanina, Tatyana and Cupovic, Jovana and Ludewig, Burkhard and Bocharov, Gennady
Journal: Journal of mathematical biology (2014): 1547-83
Quality control of extracorporeal photochemotherapy: Proliferation assay using CFSE validated according to ISO 15189:2007 standards.
Authors: Lionel, Faivre and Lucie, Lecouflet and Wang-Qing, Liu and Isabelle, Khadher and Camille, Lahaie and Michel, Vidal and Sabine, Legouvello and Jean-Louis, Beaumont and Philippe, Bierling and Hélène, Rouard and Brigitte, Birebent
Journal: Cytometry. Part B, Clinical cytometry (2014)
Asymmetry of Cell Division in CFSE-Based Lymphocyte Proliferation Analysis.
Authors: Bocharov, Gennady and Luzyanina, Tatyana and Cupovic, Jovana and Ludewig, Burkhard
Journal: Frontiers in immunology (2013): 264
说明书
Cell Meter BX590固定化细胞活性染料.pdf
