RatioWorks Cal-590L®/Cy5-葡聚糖偶联物 *MW 10,000*
 |
货号 |
20527 |
存储条件 |
在零下15度以下保存, 避免光照 |
| 规格 |
1 mg |
价格 |
5940 |
| Ex (nm) |
|
Em (nm) |
|
| 分子量 |
N/A |
溶剂 |
Water |
| 产品详细介绍 |
简要概述
产品基本信息
货号：20527
产品名称：RatioWorks Cal-590L®/Cy5-葡聚糖偶联物 *MW 10,000*
规格：1mg
储存条件：-15℃避光防潮
保质期：12个月
产品物理化学光谱特性
外观：固体
溶剂：水
产品介绍
钙测量对于许多生物学研究至关重要。在结合钙时显示光谱响应的荧光探针使研究人员能够通过使用荧光显微镜、流式细胞术、荧光光谱和荧光酶标仪来研究细胞内游离钙浓度的变化。可以使用显微注射等方式，用这些葡聚糖缀合的钙指示剂的不透细胞盐形式对细胞进行物理加载。使用荧光显微镜检测来自这些细胞的荧光信号。与AM 酯形式相比，我们的钙指示剂的葡聚糖形式表现出更少的渗漏。 RatioWorks Cal-590L/Cy5-Dextran Conjugate 携带不依赖钙的 Cy5 荧光团作为参考颜色。Cal-590L 荧光团可以被氪激光在 568 nm 处很好地激发，其中 Cy5 的激发最小。参考 Cy5 荧光团可以被 633 nm 的 He-Ne 激光或 647 nm 的红色激光很好地激发，其中 Cal-590L 荧光团的激发最小。
点击查看光谱
参考文献
In vivo nuclear Ca2+-ATPase phosphorylation triggers intermediate size molecular transport to the nucleus.
Authors: Gensburger, Claire and Freyermuth, Solange and Klein, Christian and Malviya, Anant N
Journal: Biochemical and biophysical research communications (2003): 1225-8
Real time, confocal imaging of Ca(2+) waves in arterially perfused rat hearts.
Authors: Baader, Andreas P and Büchler, Lorenz and Bircher-Lehmann, Lilly and Kléber, André G
Journal: Cardiovascular research (2002): 105-15
Assessment and validation of a microinjection method for kinetic analysis of [Ca2+]i in individual cells undergoing apoptosis.
Authors: Tombal, B and Denmeade, S R and Isaacs, J T
Journal: Cell calcium (1999): 19-28
Effects of adenosine on Ca2+ entry in the nerve terminal of the frog neuromuscular junction.
Authors: Robitaille, R and Thomas, S and Charlton, M P
Journal: Canadian journal of physiology and pharmacology (1999): 707-14
The nucleus of HeLa cell contains tubular structures for Ca2+ signalling.
Authors: Lui, P P and Kong, S K and Kwok, T T and Lee, C Y
Journal: Biochemical and biophysical research communications (1998): 88-93
The rise of nuclear and cytosolic Ca2+ can be uncoupled in HeLa cells.
Authors: Lui, P P and Kong, S K and Fung, K P and Lee, C Y
Journal: Pflugers Archiv : European journal of physiology (1998): 371-6
Porcine aortic endothelial gap junctions: identification and permeation by caged InsP3.
Authors: Carter, T D and Chen, X Y and Carlile, G and Kalapothakis, E and Ogden, D and Evans, W H
Journal: Journal of cell science (1996): 1765-73
Calcium signals in and around the nucleus in sea urchin eggs.
Authors: Gillot, I and Whitaker, M
Journal: Cell calcium (1994): 269-78
Spontaneous and propagated calcium release in isolated cardiac myocytes viewed by confocal microscopy.
Authors: Williams, D A and Delbridge, L M and Cody, S H and Harris, P J and Morgan, T O
Journal: The American journal of physiology (1992): C731-42
说明书
RatioWorks Cal-590L®/Cy5-葡聚糖偶联物 *MW 10,000*.pdf
