Portelite 荧光法RNA定量试剂盒*优化于 Cytocite 和 Qubit 荧光仪*
货号 |
17659 |
存储条件 |
Multiple |
规格 |
500 Tests |
价格 |
3732 |
Ex (nm) |
509 |
Em (nm) |
529 |
分子量 |
|
溶剂 |
|
产品详细介绍 |
简要概述
产品基本信息
货号:17659
产品名称:Portelite 荧光法RNA定量试剂盒
规格:500 Tests
储存条件:保存在冰箱-15℃干燥
保质期:12个月
成分
成分A:StrandBrite 绿色 |
0.25 mL(在DMSO中为200X) |
成分B:测定缓冲液 |
1瓶(50ml) |
成分C:RNA标准#1 |
1 mL(RNA:0 ng / µL) |
成分D:RNA标准#2 |
4 X 250 µL(RNA:10 ng / µL) |
适用仪器
Qubit 荧光计 |
|
激发: |
480nm |
发射: |
530nm |
器材: |
0.2 mL PCR小瓶 |
CytoCite 荧光计 |
|
激发: |
480nm |
发射: |
530nm |
推荐孔板: |
0.2 mL PCR小瓶 |
产品介绍
检测和定量少量RNA对于多种生物学应用极为重要,例如在进行Northern blot分析,S1核酸酶测定,RNase保护测定,cDNA文库制备,反转录之前,测量体外转录RNA的产量和测定RNA浓度。 PCR和差异显示PCR。测量核酸浓度最常用的技术是测定260 nm的吸光度。基于吸收率的方法受到蛋白质,游离核苷酸和其他紫外线吸收化合物引起的干扰的限制。敏感和选择性荧光核酸染色剂的使用减轻了这种干扰问题。StrandBrite RNA定量试剂是一种超灵敏的荧光核酸染料,用于定量溶液中的RNA。使用CytoCite 或Qubit 荧光仪,StrandBrite RNA定量试剂可检测低至5 ng / mL的RNA。我们的StrandBrite Green荧光RNA定量试剂盒包括我们的StrandBrite Green核酸染色剂,稳定性极高。它提供了一种定量溶液中RNA的简便方法。
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说明书
Portelite 荧光法RNA定量试剂盒*优化于 Cytocite 和 Qubit 荧光仪*.pdf