Screen Quest CHO-Gqo嵌合体细胞系
货号 |
38102 |
存储条件 |
在零下80度以下保存 |
规格 |
Each |
价格 |
0 |
Ex (nm) |
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Em (nm) |
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分子量 |
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溶剂 |
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产品详细介绍 |
简要概述
产品基本信息
货号:38102
产品名称:Screen Quest CHO-Gqo嵌合体细胞系
规格:Each
适用仪器
其他仪器 |
FDSS, FLIPR, ViewLux, NOVOStar, ArrayScan, FlexStation, IN Cell Analyzer |
产品介绍
Screen Quest 细胞系是一系列细胞,已成功用于药物发现和筛选环境中,用于研究通常不通过细胞内钙偶联的G蛋白偶联受体(GPCR)。它已与FLIPR,FDSS系统有效结合,与许多受体的非Gq偶联成员结合使用,例如趋化因子,5-羟色胺,谷氨酸,多巴胺,阿片样物质,血管加压素以及α和β肾上腺素受体家族。超过60%的已知GPCR信号通过Gq以外的途径导致细胞内钙的增加,并且随着基因组学揭示出更多的G蛋白偶联受体靶标,这种趋势持续增加。 Screen Quest 细胞系用于研究通常不通过细胞内钙偶联的G蛋白偶联受体(GPCR)。 Screen Quest 细胞系基于一系列G蛋白嵌合体,包括混杂的G蛋白Gα16。嵌合体由Gq蛋白复合物的α亚基组成,该复合物的5个羧基末端氨基酸已被其他G蛋白之一(Gαs,Gαi,Gαo或Gαz)取代。这些氨基酸负责受体与其G蛋白的偶联。这些嵌合体与通常通过cAMP途径起作用的特定非Gq偶联受体的共表达可能会导致受体刺激后细胞内钙信号的产生。 Screen Quest CHO-Gqo细胞系是被嵌合Gqoα亚基蛋白稳定转染的CHO-K1细胞。当用作转染表达Gi偶联受体的宿主细胞时,细胞中组成型表达的Gqo蛋白可使正常通过cAMP途径起作用的转染受体与Gq信号转导和动员的细胞内钙偶联。可以使用钙敏感染料(例如Calbryte 520 AM,Cal-520 AM,Fluo-8 AM或Fluo-4 AM)和免洗的钙试剂盒检测特定配体对这些非Gq偶联受体的激活。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的Screen Quest CHO-Gqo嵌合体细胞系。
图示
图1.用Cal-520®,AM(Cat#21130)在CHO-Ga16-NOP细胞中测量了伤害感受肽刺激的钙反应。 将CHO-Ga16-NOP细胞以60,000个细胞/ 100 µL /孔在Costar黑色96孔板中接种过夜。 在含有20 mM Hepes缓冲液(HHBS)的Hanks中,将等体积(100 µL)的10 µMCal-520®AM和2 mM丙磺舒在37°C下孵育1小时。 用具有1 mM丙磺舒的HHBS代替Cal-520®AM上样溶液。 通过FlexStation(分子设备)添加了Nociceptin,以达到最终指示的浓度。
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参考文献
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Journal: Front Pharmacol (2015): 203
说明书
Screen Quest CHO-Gqo嵌合体细胞系.pdf