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ReadiUse DRAQ5染色液 5 mM DMSO CAS 252903-95-0
货号 | 17558 | 存储条件 | 避免受潮, 在2-8度冷藏保存, 避免光照 | |
规格 | 50 uL | 价格 | 1944 | |
Ex (nm) | 601 | Em (nm) | 699 | |
分子量 | 485.41 | 溶剂 | Water | |
产品详细介绍 |
简要概述
产品基本信息
货号:17558
产品名称:ReadiUse DRAQ5染色液
CAS:252903-95-0
规格:50ul
储存条件:-15℃避光干燥
保质期:12个月
产品物理化学光谱特性
分子量:485.41
溶剂:水
激发波长(nm):646
发射波长(nm):697
产品介绍
DRAQ5 是1,5-双{[2-(二甲基氨基)乙基]氨基} -4,8-二羟基蒽-9,10-二酮的商标名(DRAQ-5 是Biostatus Ltd.的商标) 。它是活细胞或固定细胞的远红色DNA染色剂,可以很容易地与其他荧光团(例如表达绿色荧光蛋白(GFP)融合蛋白的细胞)复用。它是亲脂性和膜渗透性染料,用于活细胞和固定细胞染色以及DNA含量定量。它也是与其他荧光报告基因一起用于荧光显微镜或高含量筛选(HCS)的理想选择。由于其远红外激发和发射,DRAQ-5染色剂可与许多其他荧光团多路复用。在涉及表达绿色荧光蛋白(GFP)融合蛋白的细胞的细胞增殖研究中,通常选择它来复核核并测量DNA含量。DRAQ-5与广泛的仪器平台上的许多操作方案。AAT Bioquest提供了此即用型实验方案,可帮助您进行荧光成像和流式细胞术测试。
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参考文献
DRAQ5 and Eosin (‘D&E’) as an Analog to Hematoxylin and Eosin for Rapid Fluorescence Histology of Fresh Tissues
Authors: K. N. Elfer
Journal: PLoS One (2016): e0165530
One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays
Authors: Sheng Dai, Rong Li, Yan Long, Steve Titus, Jinghua Zhao, Ruili Huang, Menghang Xia, Wei Zheng
Journal: Journal of Biomolecular Screening (2016): 1112–1124
Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production
Authors: Guoqiang Zhang, Yuping Lin, Xianni Qi, Lixian Wang, Peng He, Qinhong Wang, Yanhe Ma
Journal: Microbial cell factories (2015): 1
Short exposure to the DNA intercalator DRAQ5 dislocates the transcription machinery and induces cell death
Authors: E. Richard
Journal: Photochem Photobiol (2011): 256-61
Influence of the live cell DNA marker DRAQ5 on chromatin-associated processes
Authors: P. O. Mari
Journal: DNA Repair (Amst) (2010): 848-55
The use of DRAQ5 to monitor intracellular DNA in Escherichia coli by flow cytometry
Authors: F. Silva
Journal: J Fluoresc (2010): 907-14
DRAQ5-based, no-lyse, no-wash bone marrow aspirate evaluation by flow cytometry
Authors: R. W. Allan
Journal: Am J Clin Pathol (2008): 706-13
DRAQ5: improved flow cytometric DNA content analysis and minimal residual disease detection in childhood malignancies
Authors: K. Swerts
Journal: Clin Chim Acta (2007): 154-7
DRAQ5-based DNA content analysis of hematolymphoid cell subpopulations discriminated by surface antigens and light scatter properties
Authors: C. M. Yuan
Journal: Cytometry B Clin Cytom (2004): 47-52
A novel cell permeant and far red-fluorescing DNA probe, DRAQ5, for blood cell discrimination by flow cytometry
Authors: Smith PJ, Wiltshire M, Davies S, Patterson LH, Hoy T.
Journal: J Immunol Methods (1999): 131