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胱天蛋白酶Caspase抑制剂Z-LETD-FMK
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货号 | 13307 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 1 mg | 价格 | 1200 | |
| Ex (nm) | Em (nm) | |||
| 分子量 | 654.69 | 溶剂 | DMSO | |
| 产品详细介绍 | ||||
简要概述
Z-LETD-FMK是一种细胞渗透性不可逆的caspase蛋白酶8抑制剂，与caspase-8的催化位点结合，在诱导凋亡中起重要作用。Z-LETD-FMK与caspase-8的结合抑制了该蛋白酶的活性，使其能够抑制与caspase-8激活相关的凋亡事件。为了抑制细胞凋亡，应在诱导细胞凋亡的同时添加Z-LETD-FMK。FMK衍生的肽可作为有效的不可逆抑制剂，而不会增加细胞毒性作用。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Z-LETD-FMK。
产品说明书
样品实验方案
以下是我们推荐的方案，仅提供指导。具体实验应根据您的特定需求进行修改。
溶液配制
储备溶液配制
操作步骤
- 根据需要处理样品以诱导凋亡。
- 添加Z-LETD-FMK。
注意：我们建议使用最终浓度为10-100 µM的Z-LETD-FMK抑制胱天蛋白酶，例如对caspase-8抑制50 µM。
注意：孵育的最佳时间和浓度需要通过实验确定。 - 根据需要对样品染色。
- 用适当的仪器检测荧光强度。
图示
 图1.用caspase 8抑制剂Z-LETD-FMK（Cat＃13307）抑制Jurkat细胞中caspase 8活性。当天，将Jurkat细胞以200,000个细胞/ 90 µL /孔的密度接种在Costar黑色96孔板中。用1 µM星形孢菌素处理细胞5小时。加入Z-LETD-FMK并与星形孢菌素共孵育。用Cell Meter Caspase 8活性细胞凋亡检测试剂盒（Cat#22816）检测caspase-8活性。用Flexstation 3荧光酶标仪（Molecular Devices）在Ex / Em ＝ 540 / 620nm（截止＝ 610nm）下测量荧光强度。 |
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