山羊抗人IgG（Fc）血清

简要概述

使用纯化的人IgG Fc片段作为免疫原，可在山羊中产生抗人IgG（Fc特异性）抗血清。当针对纯化的人IgA，IgG（Fc和Fab片段）进行测试时，抗体制剂对人IgG，Fc片段具有特异性。缺乏与小鼠或大鼠血清蛋白的种间交叉反应性，使得该产品非常适合筛选由在小鼠或大鼠腹水中体内生长的杂交瘤细胞产生的人单克隆抗体。该产品具有检测正常或病理情况（例如癌症或自身免疫性疾病）的人类生物体液或组织中所有人类IgG亚类的能力。它可以有效地用作免疫分析程序中的二级抗体试剂，并且可以用作使用酶或荧光染料的偶联物的起始材料。山羊生产的抗人IgG（Fc特异性）抗体已用于双捕获ELISA中，以测量用CD52单克隆抗体（CAMPATH-1G）治疗的移植患者血清中的抗球蛋白反应，检测类风湿关节炎患者，小肠的IgG水平活组织检查和急性心肌梗死患者的免疫印迹分析。

参考文献

Monoclonal secondary reagents do not outperform polyclonal secondary reagents for detection of anti-HLA IgG using single antigen flow beads assays.
Authors: Bonnet, Marie and Visentin, Jonathan and Caillat-Zucman, Sophie and Jollet, Isabelle and Taupin, And Jean-Luc
Journal: HLA (2020)

An infrared IgG immunoassay based on the use of a nanocomposite consisting of silica coated Fe3O4 superparticles.
Authors: Wang, Kexin and Ding, Yadan and Hong, Xia and Liu, Yichun
Journal: Mikrochimica acta (2019): 99

Immunoglobulin G-Encapsulated Gold Nanoclusters as Fluorescent Tags for Dot-Blot Immunoassays.
Authors: Zhuang, Quan-Quan and Deng, Hao-Hua and He, Shao-Bin and Peng, Hua-Ping and Lin, Zhen and Xia, Xing-Hua and Chen, Wei
Journal: ACS applied materials & interfaces (2019): 31729-31734

A rapid point-of-care test for dengue virus-1 based on a lateral flow assay with a near-infrared fluorescent dye.
Authors: Chen, Lin and Wang, Huagui and Guo, Tongsheng and Xiao, Chaohui and Liu, Licheng and Zhang, Xiaoguang and Liu, Bo and Li, Peiran and Liu, Aixia and Li, Bo and Li, Boan and Mao, Yuanli
Journal: Journal of immunological methods (2018): 23-27

Assembly of Multifunctionalized Gold Nanoparticles with Chemiluminescent, Catalytic, and Immune Activity for Label-Free Immunoassays.
Authors: Huang, Yao and Gao, Lingfeng and Cui, Hua
Journal: ACS applied materials & interfaces (2018): 17040-17046

A New Enzyme-Linked Immunosorbent Assay for a Total Anti-T Lymphocyte Globulin Determination: Development, Analytical Validation, and Clinical Applications.
Authors: Montagna, Michela and La Nasa, Giorgio and Bernardo, Maria E and Piras, Eugenia and Avanzini, Maria A and Regazzi, Mario and Locatelli, Franco
Journal: Therapeutic drug monitoring (2017): 282-289

A novel Time-resolved Fluoroimmunoassay for the quantitative detection of Antibodies against the Phospholipase A2 Receptor.
Authors: Huang, Biao and Wang, Liang and Zhang, Yi and Zhang, Jue and Zhang, Qiuhua and Xiao, Hualong and Zhou, Bin and Sun, Zhuxing and Cao, Ya-Nan and Chen, Yu and Hu, Zhigang and Sheng, Huiming
Journal: Scientific reports (2017): 46096

Naked-eye sensitive ELISA-like assay based on gold-enhanced peroxidase-like immunogold activity.
Authors: Wang, Shasha and Chen, Zhaopeng and Choo, Jaebum and Chen, Lingxin
Journal: Analytical and bioanalytical chemistry (2016): 1015-22

Simultaneous detection of platelet-specific antibodies based on a photonic crystal-encoded suspension array.
Authors: Kong, Xin and Ye, Baofen and Yang, Zixue and Chen, Baoan and Ling, Yun
Journal: Clinica chimica acta; international journal of clinical chemistry (2016): 72-7

A capillary based chemiluminscent multi-target immunoassay.
Authors: Cao, Yuan-Cheng
Journal: Journal of fluorescence (2015): 563-8