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FIASH二乙酸酯
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货号 | 22334 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 100 Tests | 价格 | 4836 | |
| Ex (nm) | 498 | Em (nm) | 517 | |
| 分子量 | 748.56 | 溶剂 | DMSO | |
| 产品详细介绍 | ||||
简要概述
产品基本信息
货号：22334
产品名称：FIASH二乙酸酯
规格：100 Tests
储存条件：-20℃干燥
保质期：12个月
产品物理化学光谱特性
外观：固体
分子量：784.56
溶剂：DMSO
Ex(nm):498
Em(nm):517
适用仪器
| 荧光显微镜 | |
| 激发： | FITC滤波片组 |
| 发射： | FITC滤波片组 |
| 推荐孔板： | 黑色透明 |
产品介绍
FlASH 二乙酸酯是FlASH的可渗透细胞的形式，它是一种荧光素衍生物，经修饰可包含两个彼此相距一定距离的砷原子。它由Roger Tsien及其同事在1998年开发。双砷标记技术通过砷与硫醇的高亲和力相互作用起作用。当FlASH结合四半胱氨酸（TC）序列时，其双砷基与Cys-Cys部分快速反应，并且标签变为绿色的高度荧光。双砷标记试剂FIASH是用于标记包含具有Cys-Cys-X1-X2-Cys-Cys氨基酸序列的六氨基酸基序的蛋白质的最小表达标签。最常用的四半胱氨酸是六个氨基酸的Cys-Cys-Pro-Gly-Cys-Cys序列。由于此序列很少出现在内源蛋白中，将序列整合到靶蛋白中会产生一个小的但高度特异性的蛋白标记靶。当与包含四半胱氨酸基序Cys-Cys-Pro-Gly-Cys-Cys的重组蛋白结合时，FIASH会产生强烈的绿色荧光信号。它可以用于检测蛋白质的定位，周转和运输，受体信号传导和内在化。
参考文献
Fluorescent labelling of membrane fatty acid transporter CD36 (SR-B2) in the extracellular loop.
Authors: Liu, Yilin and Rodriguez-Calvo, Ricardo and Wang, Shujin and Zhu, Xiaoqing and Broers, Jos L V and Glatz, Jan F C and Luiken, Joost J F P and Neumann, Dietbert
Journal: PloS one (2019): e0210704
Probing Arrestin Function Using Intramolecular FlAsH-BRET Biosensors.
Authors: Strungs, Erik G and Luttrell, Louis M and Lee, Mi-Hye
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 309-322
Probing the Effect of Sildenafil on Progesterone and Testosterone Production by an Intracellular FRET/BRET Combined Approach.
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Journal: Biochemistry (2019): 799-808
Using Tetracysteine-Tagged TDP-43 with a Biarsenical Dye To Monitor Real-Time Trafficking in a Cell Model of Amyotrophic Lateral Sclerosis.
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The novel mitochondria localization of influenza A virus NS1 visualized by FlAsH labeling.
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Journal: FEBS open bio (2017): 1960-1971
Universal genetic assay for engineering extracellular protein expression.
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Localization of Aggregatibacter actinomycetemcomitans cytolethal distending toxin subunits during intoxication of live cells.
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Journal: Infection and immunity (2012): 2761-70
Superresolution imaging of HIV in infected cells with FlAsH-PALM.
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Journal: Proceedings of the National Academy of Sciences of the United States of America (2012): 8564-9