产品基本信息

货号：6909

产品名称：小沟结合物探针CDPI3马来酰亚胺

规格：1 mg

储存条件：-20℃干燥

保质期：12个月

产品物理化学光谱特性

外观：固体

分子量：834.94

溶剂：DMSO

产品介绍

小沟结合物（MGB）分子选择性地结合到DNA分子的小沟，即DNA螺旋中的浅沟。MGB标记的寡核苷酸与互补的DNA和RNA靶序列形成稳定的杂合复合物。天然产物CC-1065的类似物二氢吡咯并吲哚三肽（CDPI3）已表现出与寡核苷酸连接时可在单链DNA上阻止序列特异性引物延伸。包含CDPI3的寡核苷酸可提高特异性和杂交强度，从而增强DNA双链体的稳定性。将MGB分子添加到DNA探针可以设计出较短的杂交探针，该探针可用于分子诊断，例如基于定量PCR的测定。可能有几种设计，包括Taqman MGB探针。AAT Bioquest提供此CPG支持，可将MGB部分方便地添加到寡核苷酸的3’末端。我们还提供MGB亚磷酰胺，用于用MGB部分标记5’末端。

参考文献

Synthesis and biological evaluation of a novel betulinic acid derivative as an inducer of apoptosis in human colon carcinoma cells (HT-29).
Authors: Chakraborty, Biswajit and Dutta, Debasmita and Mukherjee, Sanjukta and Das, Supriya and Maiti, Nakul C and Das, Padma and Chowdhury, Chinmay
Journal: European journal of medicinal chemistry (2015): 93-105

Novel metal-based pharmacologically dynamic agents of transition metal(II) complexes: designing, synthesis, structural elucidation, DNA binding and photo-induced DNA cleavage activity.
Authors: Raman, N and Jeyamurugan, R and Sakthivel, A and Mitu, L
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2010): 88-97

Synthesis, crystal structure, DNA binding and photo-induced DNA cleavage activity of (S-methyl-L-cysteine)copper(II) complexes of heterocyclic bases.
Authors: Patra, Ashis K and Nethaji, Munirathinam and Chakravarty, Akhil R
Journal: Journal of inorganic biochemistry (2007): 233-44

Metal-assisted light-induced DNA cleavage activity of 2-(methylthio)phenylsalicylaldimine Schiff base copper(II) complexes having planar heterocyclic bases.
Authors: Reddy, Pattubala A N and Santra, Bidyut K and Nethaji, Munirathinam and Chakravarty, Akhil R
Journal: Journal of inorganic biochemistry (2004): 377-86

Syntheses, crystal structures, and oxidative DNA cleavage of some Cu(II) complexes of 5-amino-3-pyridin-2-yl-1,2,4-triazole.
Authors: Ferrer, Sacramento and Ballesteros, Rafael and Sambartolomé, Ana and González, Marta and Alzuet, Gloria and Borrás, Joaquín and Liu, Malva
Journal: Journal of inorganic biochemistry (2004): 1436-46