Screen Quest 活细胞cAMP检测（含细胞系）

	货号	36384	存储条件	在零下15度以下保存, 避免光照
	规格	1000 Tests	价格	60588
	Ex (nm)	490	Em (nm)	520
	分子量		溶剂
	产品详细介绍

简要概述

产品基本信息

货号：36384

产品名称：Screen Quest cAMP活细胞检测（含细胞系）

规格：1000 Tests

储存条件：-15℃避光防潮

保质期：24个月

产品物理化学光谱特性

激发波长(nm)：490

发射波长(nm)：520

产品介绍

钙通量（通过Gqpathway偶联）测定法是药物发现中筛选GPCR靶标的首选方法。但是，超过60％的已知GPCR通过与cAMP偶联的腺苷酸环化酶活性发出信号。大多数现有的cAMP分析不仅需要细胞裂解，而且缺乏时间和空间分辨率。 Screen Quest 活细胞cAMP检测试剂盒以高通量形式对细胞内cAMP的变化进行实时检测，而无需细胞裂解步骤。该检测法通过包含外源性环状核苷酸门控通道（CNGC）或混杂G蛋白Gα16的细胞系进行。该通道被升高的细胞内cAMP水平激活，导致离子通量和细胞膜去极化，可以用荧光钙（例如Calbryte 520 AM，Cal-520 AM，Fluo-8 AM或Fluo-4 AM和相应的钙离子，免洗洗钙试剂盒）或荧光膜电位染料进行钙信号的检测。 Gα16与特定的非Gq偶联受体的共表达将在受体刺激后导致细胞内钙信号的产生。 Screen Quest 活细胞cAMP检测试剂盒提供了细胞系和试剂，可通过FLIPR，FDSS或其他等效的荧光酶标仪来检测细胞内cAMP的变化，它已成功用于检测Gs和Gi偶联的GPCR活性。

图示

图1. Screen Quest 活细胞cAMP检测原理

参考文献

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