上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品，欢迎访问AAT Bioquest荧光染料官网了解更多信息。
钙离子荧光探针Cal-520FF, AM
| 货号 | 21142 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 1 mg | 价格 | 6564 |
| Ex (nm) | 493 | Em (nm) | 515 |
| 分子量 | 1138.92 | 溶剂 | DMSO |
| 产品详细介绍 | |||
简要概述
产品基本信息
货号：21142
产品名称：钙离子荧光探针Cal-520FF, AM
规格：1mg
储存条件：-15℃避光防潮
保质期：24个月
产品物理化学光谱特性
分子量：1138.92
溶剂：DMSO
激发波长(nm)：492
发射波长(nm)：514
适用仪器
| 荧光显微镜 | |
| 激发： | FITC |
| 发射： | FITC |
| 推荐孔板： | 黑色透明 |
| 荧光酶标仪 | |
| 激发： | 490nm |
| 发射： | 525nm |
| cutoff: | 515nm |
| 推荐孔板： | 黑色透明 |
| 读取模式： | 底读模式 |
产品介绍
钙离子荧光探针Cal-520FF, AM是美国AAT Bioquest生产的钙离子荧光探针，Cal-520®提供了一种功能强大的基于荧光的均质检测工具，用于检测细胞内钙的动员。Cal-520®AM是一种新型的荧光钙敏感染料，与现有的绿色钙指示剂（如Fluo-3 AM和Fluo-4 AM）相比，信噪比和细胞内滞留性显着提高。表达通过钙信号的感兴趣的GPCR或钙通道的细胞可以预装可穿过细胞膜的Cal-520®AM。一旦进入细胞内，Cal-520 AM的亲脂性封闭基团就会被酯酶裂解，从而产生带负电荷的荧光染料，并留在细胞内部。与钙结合后，其荧光大大增强。当细胞被激动剂刺激时，该受体发出信号释放细胞内钙，可以显着提高Cal-520®的荧光。Cal-520®AM具有长波长，高灵敏度和> 100倍荧光增强的特性，使其成为测量细胞钙的理想指示剂。高信噪比和更好的细胞内滞留性使Cal-520®钙测定成为评估GPCR和钙通道靶标以及筛选其激动剂和拮抗剂的强大工具。与其他Cal-520®指标相比，Cal-520FF 对钙的亲和力最低，Kd〜10 uM。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的钙离子荧光探针Cal-520FF, AM。
点击查看光谱
点击查看实验方案
钙离子篇：时间轴式讲解应用于钙离子检测的探针
参考文献
A Critical Period for the Rapid Modification of Synaptic Properties at the VPm Relay Synapse
Authors: Libiao Pan, Junhua Yang, Qian Yang, Xiaomeng Wang, Liya Zhu, Yali Liu, Huifang Lou, Chou Xu, Ying Shen, Hao Wang
Journal: Frontiers in molecular neuroscience (2017)
Advances in Two-Photon Scanning and Scanless Microscopy Technologies for Functional Neural Circuit Imaging
Authors: Simon R Schultz, Caroline S Copeland, Amanda J Foust, Peter Quicke, Renaud Schuck
Journal: Proceedings of the IEEE (2017): 139–157
Bidirectional communication between sensory neurons and osteoblasts in an in vitro co-culture system
Authors: Daisuke Kodama, Takao Hirai, Hisataka Kondo, Kazunori Hamamura, Akifumi Togari
Journal: FEBS Letters (2017)
Ca 2+ signals initiate at immobile IP 3 receptors adjacent to ER-plasma membrane junctions
Authors: Nagendra Babu Thillaiappan, Alap P Chavda, Stephen C Tovey, David L Prole, Colin W Taylor
Journal: Nature Communications (2017): 1505
Calcium Imaging Across Large Areas of Intact Vascular Endothelium Reveals Stimulus-Specific Sensory Cells
Authors: Matthew D Lee, Calum Wilson, John G McCarron
Journal: The FASEB Journal (2017): 1005–8
Calcium transient assays for compound screening with human iPSC-derived cardiomyocytes: Evaluating new tools
Authors: Neil J Daily, Radleigh Santos, Joseph Vecchi, Pinar Kemanli, Tetsuro Wakatsuki
Journal: Journal of evolving stem cell research (2017): 1
Characterization of postsynaptic calcium signals in the pyramidal neurons of anterior cingulate cortex
Authors: Xu-Hui Li, Qian Song, Tao Chen, Min Zhuo
Journal: Molecular Pain (2017): 1744806917719847
Direct measurement of TRPV4 and PIEZO1 activity reveals multiple mechanotransduction pathways in chondrocytes
Authors: M Rocio Servin-Vences, Mirko Moroni, Gary R Lewin, Kate Poole
Journal: eLife (2017): e21074
Expression of the GluA2 subunit of glutamate receptors is required for the normal dendritic differentiation of cerebellar Purkinje cells
Authors: Masahiko Tanaka, Tomomi Senda, Naohide Hirashima
Journal: Neuroscience Letters (2017)
Extensive Ca 2+ leak through K4750Q cardiac ryanodine receptors caused by cytosolic and luminal Ca 2+ hypersensitivity
Authors: Akira Uehara, Takashi Murayama, Midori Yasukochi, Michael Fill, Minoru Horie, Toru Okamoto, Yoshiharu Matsuura, Kiyoko Uehara, Takahiro Fujimoto, Takashi Sakurai
Journal: The Journal of general physiology (2017): 199–218
相关产品
| 产品名称 | 货号 |
| 钙离子荧光探针Cal-520 , AM | Cat#21130 |
| 钙离子荧光探针Fluo-8, AM | Cat#21080 |
| 新型钙离子荧光探针Calbryte 520, AM *细胞渗透性* | Cat#20650 |