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AF 488-dUTP * 1 mM的Tris缓冲液(pH 7.5)*
货号 | 17040 | 存储条件 | 在零下15度以下保存, 避免光照 | |
规格 | 25 nmoles | 价格 | 2388 | |
Ex (nm) | 499 | Em (nm) | 520 | |
分子量 | 1036.99 | 溶剂 | Water | |
产品详细介绍 |
简要概述
产品基本信息
货号:17040
产品名称:AF 488-dUTP
规格:25 nmoles
储存条件:-15℃避光防潮
保质期:24个月
产品物理化学光谱特性
分子量:1036.99
溶剂:水
激发波长(nm):499
发射波长(nm):520
产品介绍
染料修饰的脱氧尿苷5′-三磷酸酯是通过常规酶掺入方法(例如反转录,缺口翻译,随机引物标记或PCR)生产染料标记DNA的最常用方法之一。 这种酶促荧光标记方法广泛用于FISH探针和基于微阵列的实验。 该AF 488-dUTP缀合物具有与ChromaTide Alexa Fluor 488-5-dUTP相同的化学结构。它与SpectrumGreen 滤光片组一起用作绿色荧光。 它提供了具有高光稳定性且不受pH值影响的明亮信号。 ChromaTide 和Alexa Fluor 是ThermoFisher的商标,SpectrumGreen 是Vysis的商标。
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参考文献
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Authors: Kim, Jung-Lye and Reader, Brenda F and Dumond, Curtis and Lee, Yonggyu and Mokadam, Nahush A and Black, Sylvester M and Whitson, Bryan A
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Peptide amphiphile nanofiber delivery of sonic hedgehog protein to reduce smooth muscle apoptosis in the penis after cavernous nerve resection.
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Visualization of heavy ion tracks by labeling 3′-OH termini of induced DNA strand breaks.
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Journal: Journal of radiation research (2011): 433-40
A novel screening method for competitive FRET-aptamers applied to E. coli assay development.
Authors: Bruno, John G and Carrillo, Maria P and Phillips, Taylor and Andrews, Carrie J
Journal: Journal of fluorescence (2010): 1211-23
Competitive FRET-aptamer-based detection of methylphosphonic acid, a common nerve agent metabolite.
Authors: Bruno, John G and Carrillo, Maria P and Phillips, Taylor and Vail, Neal K and Hanson, Douglas
Journal: Journal of fluorescence (2008): 867-76
Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection.
Authors: Bruno, John G and Carrillo, Maria P and Phillips, Taylor
Journal: Journal of biomolecular techniques : JBT (2008): 109-15