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Portelite 荧光法RNA定量试剂盒*优化于 Cytocite™ 和 Qubit™ 荧光仪*
| 货号 | 17658 | 存储条件 | Multiple |
| 规格 | 100 Tests | 价格 | 1200 |
| Ex (nm) | 509 | Em (nm) | 529 |
| 分子量 | 溶剂 | ||
| 产品详细介绍 | |||
简要概述
产品基本信息
货号：17658
产品名称：Portelite 荧光法RNA定量试剂盒
规格：100 Tests
储存条件：保存在冰箱-15℃干燥
保质期：12个月
成分
| 成分A：StrandBrite 绿色 | 0.25 mL（在DMSO中为200X） |
| 成分B：测定缓冲液 | 1瓶（50ml） |
| 成分C：RNA标准＃1 | 1 mL（RNA：0 ng / µL） |
| 成分D：RNA标准＃2 | 4 X 250 µL（RNA：10 ng / µL） |
适用仪器
| Qubit 荧光计 | |
| 激发： | 480nm |
| 发射： | 530nm |
| 器材： | 0.2 mL PCR小瓶 |
| CytoCite 荧光计 | |
| 激发： | 480nm |
| 发射： | 530nm |
| 推荐孔板： | 0.2 mL PCR小瓶 |
产品介绍
检测和定量少量RNA对于多种生物学应用极为重要，例如在进行Northern blot分析，S1核酸酶测定，RNase保护测定，cDNA文库制备，反转录之前，测量体外转录RNA的产量和测定RNA浓度。 PCR和差异显示PCR。测量核酸浓度最常用的技术是测定260 nm的吸光度。基于吸收率的方法受到蛋白质，游离核苷酸和其他紫外线吸收化合物引起的干扰的限制。敏感和选择性荧光核酸染色剂的使用减轻了这种干扰问题。StrandBrite RNA定量试剂是一种超灵敏的荧光核酸染料，用于定量溶液中的RNA。使用CytoCite 或Qubit 荧光仪，StrandBrite RNA定量试剂可检测低至5 ng / mL的RNA。我们的StrandBrite Green荧光RNA定量试剂盒包括我们的StrandBrite Green核酸染色剂，稳定性极高。它提供了一种定量溶液中RNA的简便方法。
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