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Cell Meter BX520固定化细胞活性染料
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货号 | 22510 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 200 Tests | 价格 | 2472 | |
| Ex (nm) | 491 | Em (nm) | 516 | |
| 分子量 | 溶剂 | DMSO | ||
| 产品详细介绍 | ||||
简要概述
产品基本信息
货号：22510
产品名称：Cell Meter BX520固色活性染料
规格：200 Tests
储存条件：保存在冰箱-15℃干燥
保质期：12个月
产品物理化学光谱特性
分子量：N/A
激发波长(nm)：491
发射波长(nm)：510
适用仪器
| 流式细胞仪 | |
| 激发： | 480nm激光 |
| 发射： | 530/30nm滤波片 |
| 滤波片： | FITC滤波片组 |
| 荧光显微镜 | |
| 激发： | FITC滤波片组 |
| 发射： | FITC滤波片组 |
| 推荐孔板： | 黑色透明 |
产品介绍
从活细胞中区分和排除死细胞可以更清晰地分离和鉴定细胞群,Cell Meter BX520固定化细胞活性染料是细胞不可渗透的一类荧光活性染料，它们通过优化以匹配常见流式细胞仪的主要激发激光，例如350、405、488、633和647 nm。这些染料不渗入活细胞，但渗入膜受损的细胞。它们与含胺和硫醇的蛋白质以及其他细胞成分发生不可逆的反应。由于具有受损膜的死细胞或固定细胞更容易与Cell Meter BX520固定化细胞活性染料发生反应，因此比具有完整膜的活细胞的染色更亮，这些染料可用于评估哺乳动物细胞的活与死状态。使用这些染料时，需要考虑一些因素，例如，每种染料的滴定，以确保活细胞几乎没有染色。Cell Meter BX520固定化细胞活性染料通过优化，可以在488 nm的蓝色激发光中激发，并在520 nm（FITC通道）发射。与其他商业上类似的活性染料相比，这种可固定的活性染料更加耐用、稳定。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的Cell Meter BX520固定化细胞活性染料。
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图示
 图1.通过Cell Meter 固定化细胞活性染料检测Jurkat细胞活性。处理Jurkat细胞并用Cell Meter BX520（Cat＃22510）染色，然后固定在3.7％甲醛中并通过流式细胞仪进行分析。带有FITC通道的死细胞群体（绿色）很容易与活细胞群体（红色）区分开，在固定之前和之后都获得了几乎相同的结果。 |
参考文献
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