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FluoroQuest 防褪色固定介质（含DAPI）
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货号 | 20005 | 存储条件 | 在2-8度冷藏保存, 避免光照 |
| 规格 | 20 mL | 价格 | 2472 | |
| Ex (nm) | 359 | Em (nm) | 457 | |
| 分子量 | 溶剂 | Water | ||
| 产品详细介绍 | ||||
简要概述
具有DAPI的FluoroQuest 是一种水性固定介质，用于保留组织和细胞涂片的荧光。这种封固剂含有抗褪色剂以降低染料的光漂白速率，并以DAPI强化，DAPI是DNA的复染剂。用于原位杂交技术或需要荧光法进行DNA染色的其他方法。DAPI在360 nm处激发并在460 nm处发射，产生蓝色荧光。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的FluoroQuest 防褪色固定介质。
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产品说明书
样品实验方案
以下是我们推荐的方案，仅提供指导。具体实验应根据您的特定需求进行修改。
- 预热溶液：从冰箱中取出安装溶液。将瓶子加热至室温，避光。
- 添加溶液：清除样品中多余的液体。向样品中加一滴固定溶液。如果样品在载玻片或组织培养皿上，请小心地将盖玻片放在液滴上，以免产生气泡。如果样品在盖玻片上，请将盖玻片倒在干净的玻璃载玻片上。去除多余的防褪色成分。
- 准备用于成像的样品：固定溶液应孵育2小时至过夜。要长期存放，请用指甲油或塑料密封剂将盖玻片密封在玻片上。已安装的载玻片应在黑暗中于4°C存放，荧光成像将保持稳定数周，样品可以在安装后立即成像。
图示
图1. 甲醛固定，石蜡包埋的人肺腺癌阳性组织的荧光IHC 。用兔抗EpCam抗体对人肺腺癌阳性组织切片进行染色，然后分别与iFluor 488 Styramide （Cat＃45020）染色的聚HRP标记的羊抗兔IgG二抗一起孵育。将组织固定在带有DAPI（Cat#20005）的FluoroQuest 抗褪色固定介质中。
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