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ReadiUse™10KD旋转柱
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货号 | 60502 | 存储条件 | 室温储存不要冷冻 |
| 规格 | 5 | 价格 | 960 | |
| Ex (nm) | Em (nm) | |||
| 分子量 | 溶剂 | |||
| 产品详细介绍 | ||||
简要概述
ReadiUse™10KD旋转柱是用于浓缩生物样品（如抗体）的一次性超滤设备。最大初始样品量为〜500 ul。它们既可以用在摆动式转子，也可以用在固定式转子中，接受2.0 g约10000 g的离心管。止动体积为〜20-25 µl。垂直的膜设计和细通道过滤室可最大程度地减少膜污染，并提供高速浓缩甚至满载的溶液。它经过专门设计，具有低内表面和膜面积，可从稀溶液中获得优异的回收率。
产品说明书
样品分析方案
详细参数
聚丙烯外壳和聚醚砜（PES）膜
MWCO：10K
样品量：25 µL-500 µL
样品回收率高
应用：蛋白质样品的浓缩，脱盐，缓冲液交换和脱蛋白
最大离心力：14,000克
推荐离心力：5,000〜10,000 g
操作步骤
将蛋白质样品加到过滤器中，以8,000〜10,000 g离心。
注意：从500 µL浓缩到〜50 µL大约需要10到15分钟。
脱盐，缓冲液交换或渗滤是去除含生物分子溶液中盐或溶剂的重要方法。可以通过以下方法在ReadiUse™10KD离心柱中去除盐或交换缓冲液：浓缩样品，然后用任何所需的溶剂将浓缩液重新配制为原始样品体积。可以重复“洗出”的过程，直到污染微溶质的浓度充分降低为止。
将细胞裂解物或组织匀浆加入滤膜，以10,000 g离心10到15min，收集洗脱液。
图1. ReadiUse™10KD旋转柱是用于浓缩生物样品（如抗体）的一次性超滤设备。最大初始样品量为〜500 ul。它们既可以用在摆动式转子中，也可以用在固定角转子中，接受2.0 g约10000 g的离心管。
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