Buccutite 聚HRP抗体偶联试剂盒

	货号	5519	存储条件	在2-8度冷藏保存, 避免光照
	规格	2×50 ug Labelings	价格	6060
	Ex (nm)		Em (nm)
	分子量		溶剂
	产品详细介绍

简要概述

在样品有限或目标分子含量极低的免疫测定中，聚-HRP-抗体结合物被认为具有最高的敏感性。 Buccutite 聚HRP抗体偶联试剂盒旨在快速制备聚HRP偶联抗体。该试剂盒提供了可通过我们专有的Buccutite FOL预激活的poly-HRP探针。用Buccutite MTA（试剂盒中提供）激活靶向抗体，以得到MTA激活的抗体。 MTA激活的抗体与FOL激活的多HRP的简单混合即可得到所需的多HRP标记的抗体偶联物。该反应在极温和的条件下进行而无需催化剂。用Buccutite Poly-HRP抗体偶联试剂盒制备的poly-HRP抗体偶联物与ELISA，western blotting，免疫组化（IHC）分析中使用的发色，荧光和化学发光HRP底物兼容。它也可以与TSA或我们的Styramide 荧光HRP底物一起使用，以超灵敏地检测低丰度生物靶标。

图示

图1.使用Buccutite Poly-HRP抗体偶联试剂盒（Cat＃5518或Cat＃5519）制备的polyHRP-山羊抗小鼠IgG偶联物生成直接ELISA曲线。将3倍系列稀释的小鼠IgG包被在96孔板上，并使用标准ELISA方法检测100uL GAM IgG-polyHRP缀合物（100ng / ml）。用TMB底物溶液（Cat＃11003）孵育5分钟检测固定的小鼠IgG，并在450 nm处读数。
蓝色：5518或5519试剂盒
红色：GXM IgG PolyHRP（供应商A）
绿色：GXM IgG-HRP（供应商B）

参考文献

Detecting cancer metastasis and accompanying protein biomarkers at single cell levels using a 3D-printed microfluidic immunoarray.
Authors: Sharafeldin, Mohamed and Chen, Tianqi and Ozkaya, Gulsum Ucak and Choudhary, Dharamainder and Molinolo, Alfredo A and Gutkind, J Silvio and Rusling, James F
Journal: Biosensors & bioelectronics (2021): 112681

Prostate Cancer Diagnosis in the Clinic Using an 8-Protein Biomarker Panel.
Authors: Jones, Abby L and Dhanapala, Lasangi and Baldo, Thaísa A and Sharafeldin, Mohamed and Krause, Colleen E and Shen, Min and Moghaddam, Shirin and Faria, Ronaldo C and Dey, Dipak K and Watson, R William and Andrawis, Ramez and Lee, Norman H and Rusling, James F
Journal: Analytical chemistry (2020)

Comparison of different methodologies and cryostat versus paraffin sections for chromogenic immunohistochemistry.
Authors: Hira, Vashendriya V V and de Jong, Annique Loncq and Ferro, Klea and Khurshed, Mohammed and Molenaar, Remco J and Van Noorden, Cornelis J F
Journal: Acta histochemica (2019): 125-134

The complementary role of insulin-like growth factor II mRNA-binding protein 3 (IMP3) in diagnosis of Hodgkin’s lymphoma.
Authors: Masoud, Rokia and Ibrahiem, Afaf and Tantawy, Dina and Eldosoky, Ibrahim
Journal: Annals of diagnostic pathology (2019): 64-68

Using magnetic beads and signal amplifiers to produce short and simple immunoassays: Application to MMP-9 detection in plasma samples.
Authors: Ben Ismail, Manel and de la Serna, Erica and Ruiz-Vega, Gisela and García-Berrocoso, Teresa and Montaner, Joan and Zourob, Mohammed and Othmane, Ali and Baldrich, Eva
Journal: Analytica chimica acta (2018): 144-154

Electrochemical immunosensor for sensitive determination of transforming growth factor (TGF) – β1 in urine.
Authors: Sánchez-Tirado, E and Martínez-García, G and González-Cortés, A and Yáñez-Sedeño, P and Pingarrón, J M
Journal: Biosensors & bioelectronics (2017): 9-14

Chemiluminometric Immunosensor for High-Sensitivity Cardiac Troponin I Employing a Polymerized Enzyme Conjugate as a Tracer.
Authors: Lim, Guei-Sam and Seo, Sung-Min and Paek, Sung-Ho and Kim, Seung-Wan and Jeon, Jin-Woo and Kim, Dong-Hyung and Cho, Il-Hoon and Paek, Se-Hwan
Journal: Scientific reports (2015): 14848

Design and construction of novel molecular conjugates for signal amplification (I): conjugation of multiple horseradish peroxidase molecules to immunoglobulin via primary amines on lysine peptide chains.
Authors: Dhawan, Subhash
Journal: Peptides (2002): 2091-8

Design and construction of novel molecular conjugates for signal amplification (II): use of multivalent polystyrene microparticles and lysine peptide chains to generate immunoglobulin-horseradish peroxidase conjugates.
Authors: Dhawan, Subhash
Journal: Peptides (2002): 2099-110

Comparison of primary rabbit kidney and MRC-5 cells and two stain procedures for herpes simplex virus detection by a shell vial centrifugation method.
Authors: Peterson, E M and Hughes, B L and Aarnaes, S L and de la Maza, L M
Journal: Journal of clinical microbiology (1988): 222-4