上海金畔生物科技有限公司代理AAT Bioquest荧光染料全线产品,欢迎访问AAT Bioquest荧光染料官网了解更多信息。
ReadiUse 预活化 PE 马来酰亚胺 [活化 R-藻红蛋白]
货号 | 2565 | 存储条件 | 避免受潮, 在2-8度冷藏保存, 避免光照 |
规格 | 1 mg | 价格 | 1140 |
Ex (nm) | 566 | Em (nm) | 574 |
分子量 | 溶剂 | Water | |
产品详细介绍 |
简要概述
产品基本信息
货号:2565
产品名称:ReadiUse 预活化 PE 马来酰亚胺 [活化 R-藻红蛋白]
规格:1mg
储存条件:2-8℃避光防潮
保质期:6个月
产品物理化学光谱特性
溶剂:水
激发波长(nm):566
发射波长(nm):574
产品介绍
ReadiUse 预活化 PE 马来酰亚胺是通过高度纯化的 R-藻红蛋白 (PE) 与 SMCC 反应制备的。 SMCC 的 NHS 基团与 PE 的赖氨酸基团反应,留下马来酰亚胺基团可与待缀合的蛋白质上的游离巯基反应。 ReadiUse 预活化 PE 马来酰亚胺经过高度纯化,可完全去除未反应的 SMCC,并冻干成粉末形式。 它可以立即使用,并且在与含巯基的靶分子(如还原型 IgG)混合后无需进一步制备即可偶联。 这种活化的藻胆蛋白可以很容易地与抗体和其他蛋白质结合,而无需使用添加的化学交联剂。 制备的偶联物保持了 PE 的光谱特性。金畔生物是AAT Bioquest的中国代理商,为您提供最优质的ReadiUse 预活化 PE 马来酰亚胺 [活化 R-藻红蛋白]。
参考文献
Chromophore attachment to phycobiliprotein beta-subunits: phycocyanobilin:cysteine-beta84 phycobiliprotein lyase activity of CpeS-like protein from Anabaena Sp. PCC7120
Authors: Zhao KH, Su P, Li J, Tu JM, Zhou M, Bubenzer C, Scheer H.
Journal: J Biol Chem (2006): 8573
Excitation energy transfer from phycobiliprotein to chlorophyll d in intact cells of Acaryochloris marina studied by time- and wavelength-resolved fluorescence spectroscopy
Authors: Petrasek Z, Schmitt FJ, Theiss C, Huyer J, Chen M, Larkum A, Eichler HJ, Kemnitz K, Eckert HJ.
Journal: Photochem Photobiol Sci (2005): 1016
Single-molecule spectroscopy selectively probes donor and acceptor chromophores in the phycobiliprotein allophycocyanin
Authors: Loos D, Cotlet M, De Schryver F, Habuchi S, Hofkens J.
Journal: Biophys J (2004): 2598
Evaluation of Tolypothrix germplasm for phycobiliprotein content
Authors: Prasanna R, Prasanna BM, Mohammadi SA, Singh PK.
Journal: Folia Microbiol (Praha) (2003): 59
Isolation and characterisation of phycobiliprotein rich mutant of cyanobacterium Synechocystis sp
Authors: Prasanna R, Dhar DW, Dominic TK, Tiwari ON, Singh PK.
Journal: Acta Biol Hung (2003): 113
Co-ordinated expression of phycobiliprotein operons in the chromatically adapting cyanobacterium Calothrix PCC 7601: a role for RcaD and RcaG
Authors: Noubir S, Luque I, Ochoa de Alda JA, Perewoska I, Tandeau de Marsac N, Cobley JG, Houmard J.
Journal: Mol Microbiol (2002): 749
Phycobiliprotein genes of the marine photosynthetic prokaryote Prochlorococcus: evidence for rapid evolution of genetic heterogeneity
Authors: Ting CS, Rocap G, King J, Chisholm SW.
Journal: Microbiology (2001): 3171
Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon
Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.
Journal: FEBS Lett (2000): 9
Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging
Authors: Triantafilou K, Triantafilou M, Wilson KM.
Journal: Cytometry (2000): 226
[Phycobiliprotein and fluorescence immunological assay]
Authors: Wu P.
Journal: Sheng Li Ke Xue Jin Zhan (2000): 82