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ProLite HisTag 蛋白凝胶染色试剂盒 *绿色荧光*
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货号 | 18010 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 10 Gels | 价格 | 4836 | |
| Ex (nm) | 498 | Em (nm) | 517 | |
| 分子量 | 溶剂 | DMSO | ||
| 产品详细介绍 | ||||
简要概述
多组氨酸标签（His-Tag）广泛用于蛋白质纯化、检测和固定。 ProLite His-Tag 蛋白凝胶染色试剂盒提供了一种快速、灵敏且高度特异性的荧光染料，用于在电泳后直接在聚丙烯酰胺凝胶中观察带有 His 标签的融合蛋白。 该试剂盒需要的动手时间很少，允许使用带有标准 FITC 滤波片的成像设备在各种凝胶类型中进行快速蛋白质表达筛选。 它直接在凝胶中检测纳克 His 标记的蛋白质，从而消除了额外的蛋白质印迹步骤。 AAT Bioquest 提供种类最多的 His-Tag 检测和纯化产品。金畔生物是AAT Bioquest的中国代理商，为您提供最优质的ProLite HisTag 蛋白凝胶染色试剂盒 *绿色荧光*。
适用仪器
| 凝胶成像仪 | |
| 激发： | 蓝色激光 |
| 发射： | 长路径绿色滤镜（SYBR 滤镜） |
产品说明书
样品实验方案
储备溶液
ProLite His Tag 蛋白凝胶染色原液
在 ProLite His Tag 蛋白凝胶染料小瓶中加入 60 µL DMSO 制成储备溶液。
注意：1 瓶原液适用于 2 块凝胶。 将未使用的储备溶液储存在 -20°C。
工作溶液
ProLite His Tag 蛋白凝胶染色工作溶液
在 30 mL PBS 中加入 30 µL XdU 储备溶液，制成 ProLite His Tag 蛋白凝胶染色工作溶液。
注意：制备足够的工作溶液，使凝胶完全浸入工作溶液中。
注意：不要重复使用工作溶液。
实验步骤
后染色方案
1.根据您的标准方案运行凝胶。
2.将凝胶放入合适的容器中。 将凝胶在固定液中固定 60 分钟。 注：40% 乙醇 + 10% 醋酸可用作固定液。
3.用超纯水清洗凝胶两次。
4.将凝胶在ProLite HisTag 蛋白凝胶染料工作溶液中孵育 60 分钟。 注意：确保凝胶浸入工作溶液中。
5.取出工作溶液并用 PBS 清洗凝胶两次。
6.立即对凝胶进行成像。
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Authors: Pan, Xiaoshu and Yang, Yu and Li, Long and Li, Xiaowei and Li, Qiang and Cui, Cheng and Wang, Bang and Kuai, Hailan and Jiang, Jianhui and Tan, Weihong
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[Cloning and expression of SmDXS2 gene in Swertia mussotii].
Authors: Li, Wen-Jing and Xiang, Bei-Bei and Sun, Yan-Xiang and Hou, Xiao-Qiang and Han, Mei-Ling and Li, Xiao-Xue and Wang, Yong and Guo, Shuo
Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (2019): 935-941
Biochemical and Functional Characterization of Mouse Mammary Tumor Virus Full-Length Pr77Gag Expressed in Prokaryotic and Eukaryotic Cells.
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Journal: Viruses (2018)
Characterization of Recombinant His-Tag Protein Immobilized onto Functionalized Gold Nanoparticles.
Authors: Torres-González, Lisa and Díaz-Ayala, Ramonita and Vega-Olivencia, Carmen A and López-Garriga, Juan
Journal: Sensors (Basel, Switzerland) (2018)
Molecular characterization of Babesia microti thioredoxin (BmTrx2) and its expression patterns induced by antiprotozoal drugs.
Authors: Huang, Jingwei and Xiong, Kang and Zhang, Houshuang and Zhao, Yanzhen and Cao, Jie and Gong, Haiyan and Zhou, Yongzhi and Zhou, Jinlin
Journal: Parasites & vectors (2018): 38
Overexpression, Purification and Functional Characterisation of Wild-Type HIV-1 Subtype C Protease and Two Variants Using a Thioredoxin and His-Tag Protein Fusion System.
Authors: Zondagh, Jake and Williams, Alison and Achilonu, Ikechukwu and Dirr, Heini W and Sayed, Yasien
Journal: The protein journal (2018): 369-379
Facile fabrication of nickel immobilized on magnetic nanoparticles as an efficient affinity adsorbent for purification of his-tagged protein.
Authors: Rashid, Zahra and Naeimi, Hossein and Zarnani, Amir-Hassan and Mohammadi, Fereshteh and Ghahremanzadeh, Ramin
Journal: Materials science & engineering. C, Materials for biological applications (2017): 670-676
Effect of His-Tag on Expression, Purification, and Structure of Zinc Finger Protein, ZNF191(243-368).
Authors: Zhao, Dongxin and Huang, Zhongxian
Journal: Bioinorganic chemistry and applications (2016): 8206854