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死细胞核染料 TWO-PRO 3 相当于TO-PRO 3 CAS 157199-63-8
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货号 | 17572 | 存储条件 | 在零下15度以下保存, 避免光照 |
| 规格 | 0.2 ml | 价格 | 2604 | |
| Ex (nm) | 642 | Em (nm) | 657 | |
| 分子量 | 671.42 | 溶剂 | DMSO | |
| 产品详细介绍 | ||||
简要概述
产品基本信息
货号：17572
产品名称：死细胞核染料 TWO-PRO 3 相当于TO-PRO 3
CAS：157199-63-8
规格：0.2ml
储存条件：-15℃避光干燥
保质期：12个月
产品物理化学光谱特性
分子量：671.42
溶剂：DMSO
激发波长(nm)：642
发射波长(nm)：661
适用仪器
| 荧光显微镜 | |
| 激发： | Cy5滤波片 |
| 发射: | Cy5滤波片 |
| 推荐孔板： | 黑色透明 |
产品介绍
TWO-PRO -1在化学上等同于TO-PRO®-1（TO-PRO®是Invitrogen的商标）。TWO-PRO -1是一种碳花青二聚体，具有类似于FITC的绿色荧光。它不具有细胞渗透性，可以很容易地与Cy5和罗丹明区别开来，作为核复染和死细胞指示剂。在不存在核酸的情况下它是非荧光的，并且在与DNA结合后会产生非常明亮的荧光信号。TWO-PRO -1可在培养细胞和石蜡切片中进行强而有选择性的核染色。同时使用细胞可渗透的Nuclear Red LCS1染料和不渗透细胞的TWO-PRO -1进行标记，可用于评估细胞活力。TWO-PRO -1和Nuclear Red 的消光系数都比结合DNA的碘化丙啶大。
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参考文献
Daptomycin exerts rapid bactericidal activity against Bacillus anthracis without disrupting membrane integrity
Authors: Xing YH, Wang W, Dai SQ, Liu TY, Tan JJ, Qu GL, Li YX, Ling Y, Liu G, Fu XQ, Chen HP.
Journal: Acta Pharmacol Sin (2014): 211
A silicon cell cycle in a bacterial model of calcium phosphate mineralogenesis
Authors: Linton KM, Tapping CR, Adams DG, Carter RD, Shore RC, Aaron JE.
Journal: Micron (2013): 419
Quantification of Candida albicans by flow cytometry using TO-PRO((R))-3 iodide as a single-stain viability dye
Authors: Kerstens M, Boulet G, Pintelon I, Hellings M, Voeten L, Delputte P, Maes L, Cos P.
Journal: J Microbiol Methods (2013): 189
A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy
Authors: Gorokhova E, Mattsson L, Sundstrom AM.
Journal: J Microbiol Methods (2012): 216
Determination of the drug-DNA binding modes using fluorescence-based assays
Authors: Williams AK, Dasilva SC, Bhatta A, Rawal B, Liu M, Korobkova EA.
Journal: Anal Biochem (2012): 66
Pulsed electromagnetic field affects intrinsic and endoplasmatic reticulum apoptosis induction pathways in MonoMac6 cell line culture
Authors: Kaszuba-Zwoinska J, Chorobik P, Juszczak K, Zaraska W, Thor PJ.
Journal: J Physiol Pharmacol (2012): 537
Transient changes in neuronal cell membrane permeability after blast exposure
Authors: Arun P, Abu-Taleb R, Valiyaveettil M, Wang Y, Long JB, Nambiar MP.
Journal: Neuroreport (2012): 342
SOCS-3 antagonizes pro-apoptotic effects of TRAIL and resveratrol in prostate cancer cells
Authors: Horndasch M, Culig Z.
Journal: Prostate (2011): 1357
The fluorescent dyes TO-PRO-3 and TOTO-3 iodide allow detection of microbial cells in soil samples without interference from background fluorescence
Authors: Henneberger R, Birch D, Bergquist P, Walter M, Anitori RP.
Journal: Biotechniques (2011): 190
Impact of mitochondriotropic quercetin derivatives on mitochondria
Authors: Biasutto L, Sassi N, Mattarei A, Marotta E, Cattelan P, Toninello A, Garbisa S, Zoratti M, Paradisi C.
Journal: Biochim Biophys Acta (2010): 189