DAPI（4′,6-二脒基-2-苯基吲哚二盐酸盐）*10mM水溶液* CAS 28718-90-3

	货号	17507	存储条件	在零下15度以下保存, 避免光照
	规格	2 mL	价格	1008
	Ex (nm)	359	Em (nm)	457
	分子量	350.25	溶剂	Water
	产品详细介绍

简要概述

产品基本信息

货号：17507

产品名称：DAPI[4,6-二脒基-2-苯基吲哚，二盐酸盐]* 10 mM水溶液*

CAS：28718-90-3

规格：2ml

储存条件：保存在冰箱-20℃干燥

保质期：6个月

产品物理化学光谱特性

分子量：350.25

溶剂：水

Ex(nm):358

Em(nm):461

产品介绍

DAPI是一种与DNA结合强烈的荧光染色剂。它广泛用于荧光显微镜。由于DAPI通过完整的细胞膜，它可用于除固定细胞外的活细胞染色。对于荧光显微镜，DAPI被紫外线激发。当与双链DNA结合时，其最大吸收在358nm，其发射最大值在461nm。DAPI的一个缺点是其排放相当广泛。DAPI也与RNA结合，但是它不像它与DNA结合时那样强烈地发荧光。 当与RNA结合时，其发射偏移至约500nm .DAPI的蓝色发射便于多重测定，因为DAPI与荧光素和绿色荧光蛋白（GFP）等绿色荧光分子或红色荧光染料之间的荧光重叠非常少。除了标记细胞核外，DAPI还用于检测细胞培养物中的支原体或病毒DNA。

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参考文献

Astrocytic expression of the CXCL12 receptor, CXCR7/ACKR3 is a hallmark of the diseased, but not developing CNS
Authors: Malte Puchert, Fabian Pelkner, Gregor Stein, Doychin N Angelov, Johannes Boltze, Daniel-Christoph Wagner, Francesca Odoardi, Alexander Flügel, Wolfgang J Streit, Jürgen Engele
Journal: Molecular and Cellular Neuroscience (2017)

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Fuqiang Guo, Jiajia Shang, Hai Zhao, Kangrong Lai, Yang Li, Zhongxiong Fan, Zhenqing Hou, Guanghao Su
Journal: Colloids and Surfaces B: Biointerfaces (2017)

Legionella pneumophila infection activates bystander cells differentially by bacterial and host cell vesicles
Authors: Anna Lena Jung, Christina Elena Herkt, Christine Schulz, Kathrin Bolte, Kerstin Seidel, Nicoletta Scheller, Alexandra Sittka-Stark, Wilhelm Bertrams, Bernd Schmeck
Journal: Scientific Reports (2017)

Peptides mimicking viral proteins of porcine circovirus type 2 were profiled by the spectrum of mouse anti-PCV2 antibodies
Authors: Ling-Chu Hung, Cheng-Yao Yang, Ivan-Chen Cheng
Journal: BMC immunology (2017): 25

Poly (ADP-ribose) polymerase inhibitors activate the p53 signaling pathway in neural stem/progenitor cells
Authors: Akiko Okuda, Suguru Kurokawa, Masanori Takehashi, Aika Maeda, Katsuya Fukuda, Yukari Kubo, Hyuma Nogusa, Tomoka Takatani-Nakase, Shujiro Okuda, Kunihiro Ueda
Journal: BMC neuroscience (2017): 14

Apoptotic effect of the selective PPARβ/δ agonist GW501516 in invasive bladder cancer cells
Authors: Adeline Péchery, Sylvie Fauconnet, Hugues Bittard, Isabelle Lascombe
Journal: Tumor Biology (2016): 1–14

Carbon nanotube–Protamine hybrid: Evaluation of DNA cell penetration
Authors: Cécile Caoduro, Raoudha Kacem, Khaoula Boukari, Fabien Picaud, Claire-Hélène Brachais, David Monchaud, Christophe Borg, Hatem Boulahdour, Tijani Gharbi, Régis Delage-Mourroux
Journal: Carbon (2016): 742–752

Identification of epigenetic factors regulating the mesenchyme to epithelium transition by RNA interference screening in breast cancer cells
Authors: Jean-Marc Gregoire, Laurence Fleury, Clara Salazar-Cardozo, Frédéric Alby, Véronique Masson, Paola Barbara Arimondo, Frédéric Ausseil
Journal: BMC cancer (2016): 700

Legionella pneumophila-Derived Outer Membrane Vesicles Promote Bacterial Replication in Macrophages
Authors: Anna Lena Jung, Cornelia Stoiber, Christina E Herkt, Christine Schulz, Wilhelm Bertrams, Bernd Schmeck
Journal: PLoS Pathog (2016): e1005592

Redox-Sensitive Citronellol–Cabazitaxel Conjugate: Maintained in Vitro Cytotoxicity and Self-Assembled as Multifunctional Nanomedicine
Authors: Peng Xue, Dan Liu, Jing Wang, Na Zhang, Jiahua Zhou, Lin Li, Weiling Guo, Mengchi Sun, Xiangfei Han, Yongjun Wang
Journal: Bioconjugate chemistry (2016): 1360–1372

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